Effect of global transcriptional regulators related to carbohydrate metabolism on organic solvent tolerance in Escherichia coli

Okochi, Mina; Kurimoto, Masaki; Shimizu, Kazunori; Honda, Hiroyuki

doi:10.1263/jbb.105.389

Cited by 16 publications

(9 citation statements)

References 29 publications

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“…The upregulation of MetR possibly relieves a temporary methionine auxotrophy caused by isopentenol stress by allowing higher levels of this amino acid to be generated. Regulators in other metabolic pathways also impact solvent tolerance; where deletion of some lead to sensitivity (e.g., arcA), others resulted in tolerance toward hexane and cyclohexane in E. coli (e.g., fnr, cya) [51]. The role of several E. coli regulators, including ArcA, has been delineated for nbutanol and isobutanol stress response [22].…”

Section: Reviewmentioning

confidence: 99%

“…Developed in Saccharomyces cerevisiae and E. coli, this method has seen broad use in obtaining tolerance traits [54,55]. Similarly, manipulation of global signaling compounds can also be used in conjunction with improved solvent-tolerance phenotypes, as was done for isobutanol tolerance using cyclic-di-GMP receptors [51]. New technologies for genome editing (e.g., large clustered regularly interspaced palindromic repeats -interference (CRISPRi) libraries [55][56][57]) and targeted gene regulation [58] allow us to target multiple genes simultaneously and provide powerful new approaches in solvent-tolerance engineering.…”

Section: Reviewmentioning

confidence: 99%

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Tolerance engineering in bacteria for the production of advanced biofuels and chemicals

Mukhopadhyay

2015

Trends in Microbiology

200

117

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During microbial production of solvent-like compounds, such as advanced biofuels and bulk chemicals, accumulation of the final product can negatively impact the cultivation of the host microbe and limit the production levels. Consequently, improving solvent tolerance is becoming an essential aspect of engineering microbial production strains. Mechanisms ranging from chaperones to transcriptional factors have been used to obtain solvent-tolerant strains. However, alleviating growth inhibition does not invariably result in increased production. Transporters specifically have emerged as a powerful category of proteins that bestow tolerance and often improve production but are difficult targets for cellular expression. Here we review strain engineering, primarily as it pertains to bacterial solvent tolerance, and the benefits and challenges associated with the expression of membrane-localized transporters in improving solvent tolerance and production.

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Section: Reviewmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

Tolerance engineering in bacteria for the production of advanced biofuels and chemicals

Mukhopadhyay

2015

Trends in Microbiology

200

117

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“…coli mutants ( Aono 1998 ; Ramos et al 2002 ; Shimizu et al 2005 ; Heipieper et al 2007 ; Okochi et al 2007 ; Okochi et al 2008a ; Okochi et al 2008b ; Torres et al 2011 ; Segura et al 2012 ). Until now, more is known about how cells respond to organic solvents, but less about how to develop tolerant strains.…”

Section: Introductionmentioning

confidence: 99%

Contributions of mutations in acrR and marR genes to organic solvent tolerance in Escherichia coli

Watanabe

Doukyu

2012

AMB Express

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The AcrAB-TolC efflux pump is involved in maintaining intrinsic organic solvent tolerance in Escherichia coli. Mutations in regulatory genes such as marR, soxR, and acrR are known to increase the expression level of the AcrAB-TolC pump. To identify these mutations in organic solvent tolerant E. coli, eight cyclohexane-tolerant E. coli JA300 mutants were isolated and examined by DNA sequencing for mutations in marR, soxR, and acrR. Every mutant carried a mutation in either marR or acrR. Among all mutants, strain CH7 carrying a nonsense mutation in marR (named marR109) and an insertion of IS5 in acrR, exhibited the highest organic solvent-tolerance levels. To clarify the involvement of these mutations in improving organic solvent tolerance, they were introduced into the E. coli JA300 chromosome by site-directed mutagenesis using λ red-mediated homologous recombination. Consequently, JA300 mutants carrying acrR::IS5, marR109, or both were constructed and named JA300 acrRIS, JA300 marR, or JA300 acrRIS marR, respectively. The organic solvent tolerance levels of these mutants were increased in the following order: JA300 < JA300 acrRIS < JA300 marR < JA300 acrRIS marR. JA300 acrRIS marR formed colonies on an agar plate overlaid with cyclohexane and p-xylene (6:4 vol/vol mixture). The organic solvent-tolerance level and AcrAB-TolC efflux pump-expression level in JA300 acrRIS marR were similar to those in CH7. Thus, it was shown that the synergistic effects of mutations in only two regulatory genes, acrR and marR, can significantly increase organic solvent tolerance in E. coli.

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“…In E. coli, global regulator CRP regulates more than 400 genes (Khankal et al 2009). When suffering n-hexane stress, the adenylate cyclase (CyaA) and CRP knockout mutants had an enhanced tolerance (Okochi et al 2008). Through engineering artificial TF or CRP libraries, butanol-tolerant strains were obtained, and a mutant stain tolerated up to 1Á5% (vol/vol) butanol, with a concomitant increase in heat resistance (Lee et al 2011;Zhang et al 2012).…”

Section: Other Regulators Related To Biofuel Tolerancementioning

confidence: 99%

Regulatory mechanisms related to biofuel tolerance in producing microbes

Chen

Zhang

2016

J Appl Microbiol

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Summary Production of renewable biofuels through either native or engineered microbes has drawn significant attention in recent years, mostly due to the increasing concerns on the energy crisis and the environmental consequences of the overutilization of petroleum‐based fuels. Although significant progress has been achieved thus far, further advances are still necessary in order to decrease the manufacturing cost so that the producing processes can be more competitive to petroleum fuels. Among various possible approaches, the increase in biofuel tolerance in microbes has been suggested as one aspect which is important for the success of biofuel production at industry‐scale. In this article, we critically summarize recent advances in deciphering regulatory mechanisms for enhancing biofuel tolerance in various micro‐organisms, focusing on functions and utilization of several well‐studied regulatory mechanisms in microbes, such as two‐component signal transduction systems, sigma factors, transcription factors, noncoding RNA and other regulators.

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Effect of global transcriptional regulators related to carbohydrate metabolism on organic solvent tolerance in Escherichia coli

Cited by 16 publications

References 29 publications

Tolerance engineering in bacteria for the production of advanced biofuels and chemicals

Tolerance engineering in bacteria for the production of advanced biofuels and chemicals

Contributions of mutations in acrR and marR genes to organic solvent tolerance in Escherichia coli

Regulatory mechanisms related to biofuel tolerance in producing microbes

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