Effect of Glimepiride on Paraoxonase Activity

Demir, Yaşar; Nadaroğlu, Hayrünnisa; Demir, Nazan

doi:10.1080/13880200600751717

Cited by 4 publications

(2 citation statements)

References 24 publications

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“…Activity measurement is based on establishing the changes in absorption of formed p-nitrophenol as a result of the reaction of PON1 with paraoxon at 412 nm. p-Nitrophenol the molar extinction coefficient is e = 18.290M −1 cm −1 and an enzyme unit is number of micromole of hydrolysis paraoxon in per minute [41][42][43].…”

Section: 22mentioning

confidence: 99%

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Synthesis and evaluation of novel 2-[(1,2,4-triazol-3-yl)thio]acetamide derivatives as potential serum paraoxonase-1 (PON1) activators

Yurttaş¹,

Küçükoğlu²,

Nadaroğlu³

et al. 2017

mpj

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Coronary artery disease and low-density lipoprotein (LDL) levels in the blood have long been known to be associated with peripheral vascular diseases. Paraoxonase-1 (PON1) enzyme is related to serum levels of high-density lipoprotein (HDL) and protects LDL from oxidation which may result in development of microvascular disease in diabetes. The enzyme has a major role in the prevention of atherosclerosis besides antioxidant properties. Additionally, PON1 is important in the detoxification of organophosphate insecticides from the body. In this study, we aimed to synthesize highly active new compounds which can be a drug candidate and evaluate their effects on PON1 activity. Nine novel triazole compounds bearing thioacetamide moiety (5a-i) were synthesized and their in vitro PON1 activity was investigated. The PON1 enzyme was purified from human serum using ammonium sulfate precipitation method. Also, it was further purified using Sepharose 4B-L-tyrosine-1-naphthylamine affinity chromatography. Among the synthesized triazole compounds, 5b, 5c, 5f and 5h have been determined to increase PON1 activity, remarkably. Compounds 5b, 5c, 5f and 5h bearing 5-nitrothiazole, benzothiazole, 6-ethoxybenzothiazole and 6-florobenzothiazole moieties could be considered to proceed in vivo investigations which is a further stage for a drug candidate.

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Section: 22mentioning

confidence: 99%

“…Human paraoxonase 1 was eluted with the solution 25 mM Tris/HCl (pH 8.0)/10 mM CaCl 2 at 0.5mL/min. Fractions (3 mL each) were collected as 3 mL, and those with the highest PON1 activity were combined [42,43].…”

Section: Sepharose 4b-l-tyrosine-1-naphthylamine Affinity Chromatographmentioning

confidence: 99%

Synthesis and evaluation of novel 2-[(1,2,4-triazol-3-yl)thio]acetamide derivatives as potential serum paraoxonase-1 (PON1) activators

Yurttaş¹,

Küçükoğlu²,

Nadaroğlu³

et al. 2017

mpj

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Investigation ofin vivoeffect of florfenicol on metabolic-antioxidant enzymes’ activities on Morkaraman normal and lactating sheep

Nadaroğlu

Küçükoğlu

Koç

2013

Journal of Taibah University for Science

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Purification of Human Serum Paraoxonase and Effect of Acetylsalicylic Acid on Paraoxonase Activity

Demir¹,

Nadaroğlu

Demir³

2008

Pharmaceutical Biology

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Paraoxonase was purified from human serum using sepharose-4B-L-tyrosine-1-napthylamine affinity chromatography. This enzyme was purified 1797-fold. SDSpolyacrylamide electrophoresis of the enzyme indicates a single protein staining band with an apparent Mr of 43 kDa. The kinetic properties of the purified enzyme were determined. The V max and K m are 231.5 µ/mg protein (EU) and 3.96 mM using substrate paraoxon respectively. The effect of acetylsalicylic acid on purified human serum paraoxonase has been investigated in vitro. The inhibition and activation effects of acetylsalicylic acid on serum paraoxonase activity were measured spectrophotometrically using paraoxon as the substrate. The phenotypic distribution of paraoxonase was determined using the dual substrate paraoxon and phenyl acetate as substrates. It was observed that acetylsalicylic acid inhibited human serum paraoxonase activity. In addition, an in vivo study was performed for acetylsalicylic acid in Sprague-Dawley rats. It was demonstrated that paraoxonase in the serum of SpragueDawley rat was activated, but activity in liver and heart was significantly inhibited.

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Effect of Glimepiride on Paraoxonase Activity

Cited by 4 publications

References 24 publications

Synthesis and evaluation of novel 2-[(1,2,4-triazol-3-yl)thio]acetamide derivatives as potential serum paraoxonase-1 (PON1) activators

Synthesis and evaluation of novel 2-[(1,2,4-triazol-3-yl)thio]acetamide derivatives as potential serum paraoxonase-1 (PON1) activators

Investigation ofin vivoeffect of florfenicol on metabolic-antioxidant enzymes’ activities on Morkaraman normal and lactating sheep

Purification of Human Serum Paraoxonase and Effect of Acetylsalicylic Acid on Paraoxonase Activity

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