Effect of gibberellic acid (GA<sub>3</sub>) addition on physiological parameters and metal uptake in <i>Phaseolus vulgaris</i> seedlings under cadmium and lead stress

Tepe, Hafize Dilek

doi:10.1080/11263504.2021.2013331

Cited by 4 publications

(4 citation statements)

References 73 publications

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“…Gibberellic acids (GAs) are one of the 5 major groups of plant hormones, along with growth hormone, cytokinin, abscisic acid, and ethylene (Keswani et al 2022 ). To date, more than 136 types of gibberellins with different structural compositions have been isolated from plants, fungi, and bacteria, and were numbered according to the order of their discovery (Dilek Tepe 2021 ). However, only a few gibberellins have known biological activities, such as GA1, GA3, GA4, and GA7 (Hedden and Thomas 2012 ).…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

Yang

Peng

et al. 2022

Bioresour. Bioprocess.

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Gibberellic acid (GA3) is a plant growth hormone that plays an important role in the production of crops, fruits, and vegetables with a wide market share. Due to intrinsic advantages, liquid fermentation of Fusarium fujikuroi has become the sole method for industrial GA3 production, but the broader application of GA3 is hindered by low titer. In this study, we combined atmospheric and room-temperature plasma (ARTP) with ketoconazole-based screening to obtain the mutant strain 3-6-1 with high yield of GA3. Subsequently, the medium composition and fermentation parameters were systematically optimized to increase the titer of GA3, resulting in a 2.5-fold increase compared with the titer obtained under the initial conditions. Finally, considering that the strain is prone to substrate inhibition and glucose repression, a new strategy of fed-batch fermentation was adopted to increase the titer of GA3 to 575.13 mg/L, which was 13.86% higher than the control. The strategy of random mutagenesis combined with selection and fermentation optimization developed in this study provides a basis for subsequent research on the industrial production of GA3. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

Yang

Peng

et al. 2022

Bioresour. Bioprocess.

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“…Phaseolus vulgaris L. (common bean) is a vital grain legume cultivated around the world, mainly in developing countries. Common beans provide multiple health benefits and are a great source of protein, starch, dietary fiber, vitamins, (thiamine, vitamin B6), folic acid, and minerals, and offer numerous health benefits to consumers [26]. According to a survey conducted in 2019, the common bean was cultivated on 33,066,183 ha land in the world and a yield of 874.0 kg/ha was attained [27].…”

Section: Introductionmentioning

confidence: 99%

“…Lead has adverse effects on P. vulgaris growth and inhibits biochemical reactions, leading to a reduction in chlorophyll content and enhancing the proline content [29]. On the other hand, Pb reduces the uptake of macro and micro elements in common beans and shows a decline in plant growth, flower development, grain development, seed germination, and common bean yield under stress of Pb [26].…”

Section: Introductionmentioning

confidence: 99%

Seed Priming with Triacontanol Alleviates Lead Stress in Phaseolus vulgaris L. (Common Bean) through Improving Nutritional Orchestration and Morpho-Physiological Characteristics

Ahmed

Amjad

Sardar

et al. 2023

Plants

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Worldwide, crop productivity is highly influenced by heavy metal toxicity. Lead (Pb) the is second-most toxic heavy metal that has high persistence in soil. Lead is translocated in plants from rhizosphere soil and enters the food chain, where it poses a significant hazard to the health of humans. In the present investigation, seed priming with triacontanol (Tria) was used to mitigate Pb phytotoxicity in Phaseolus vulgaris L. (common bean). Seeds were primed with different concentrations of Tria (control, 10 µmol L−1, 20 µmol L−1, 30 µmol L−1) solutions. The pot experiment was carried out by sowing Tria-primed seeds in contaminated soil with 400 mg kg−1 Pb. Lead alone induced a decrease in the rate of germination and a significant reduction in biomass and growth of P. vulgaris as compared to the control. All these negative effects were reversed by Tria-primed seeds. Proliferation of photosynthetic pigments was observed 1.8-fold by Tria under Pb stress. Primed seeds with 20 µmol L−1 Tria enhanced stomatal conductance (gs), photosynthetic rate (A), transpiration rate (Ei), and uptake of mineral contents (Mg+2, Zn+2, Na+, and K+) and reduced Pb accumulation in seedlings. Tria caused a 1.3-fold increase in osmotic regulator proline synthesis to alleviate Pb stress. Phenolics, soluble protein, and DPPH free radical scavenging activity were enhanced by Tria application, suggesting that exogenous Tria could be employed to improve plant tolerance to Pb stress.

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“…They have been numbered based on their order of identification. However, only a few gibberellins, such as GA 1 , GA 3 , GA 4 , and GA 7 , are known to possess biological activities . Among them, GA 3 has attracted the most attention due to its strong activity and wide application prospects for regulating the dormancy of plants as well as promoting the growth of stems and fruits. , Therefore, GA 3 was applied in many fields, including agriculture, forestry, and the brewing industry, with good economic value and social benefits …”

Section: Introductionmentioning

confidence: 99%

Improving Gibberellin GA₃ Production with the Construction of a Genome-Scale Metabolic Model of Fusarium fujikuroi

Li,

Qian,

Huang

et al. 2023

J. Agric. Food Chem.

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Liquid fermentation is the primary method for GA 3 production usingFusarium fujikuroi. However, production capacity is limited due to unknown metabolic pathways. To address this, we constructed a genome-scale metabolic model (iCY1235) with 1753 reactions, 1979 metabolites, and 1235 genes to understand the GA 3 regulation mechanisms. The model was validated by analyzing growth rates under different glucose uptake rates and identifying essential genes. We used the model to optimize fermentation conditions, including carbon sources and dissolved oxygen. Through the OptForce algorithm, we identified 20 reactions as targets. Overexpressing FFUJ_02053 and FFUJ_14337 resulted in a 37.5 and 75% increase in GA3 titers, respectively. These targets enhance carbon flux toward GA 3 production. Our model holds promise for guiding the metabolic engineering of F. fujikuroi to achieve targeted overproduction. In summary, our study utilizes the iCY1235 model to understand GA 3 regulation, optimize fermentation conditions, and identify specific targets for enhancing GA 3 production through metabolic engineering.

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Effect of gibberellic acid (GA₃) addition on physiological parameters and metal uptake in Phaseolus vulgaris seedlings under cadmium and lead stress

Cited by 4 publications

References 73 publications

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

Seed Priming with Triacontanol Alleviates Lead Stress in Phaseolus vulgaris L. (Common Bean) through Improving Nutritional Orchestration and Morpho-Physiological Characteristics

Improving Gibberellin GA₃ Production with the Construction of a Genome-Scale Metabolic Model of Fusarium fujikuroi

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Effect of gibberellic acid (GA3) addition on physiological parameters and metal uptake in Phaseolus vulgaris seedlings under cadmium and lead stress

Cited by 4 publications

References 73 publications

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

Seed Priming with Triacontanol Alleviates Lead Stress in Phaseolus vulgaris L. (Common Bean) through Improving Nutritional Orchestration and Morpho-Physiological Characteristics

Improving Gibberellin GA3 Production with the Construction of a Genome-Scale Metabolic Model of Fusarium fujikuroi

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Effect of gibberellic acid (GA₃) addition on physiological parameters and metal uptake in Phaseolus vulgaris seedlings under cadmium and lead stress

Improving Gibberellin GA₃ Production with the Construction of a Genome-Scale Metabolic Model of Fusarium fujikuroi