Effect of genotype, explant size, position, and culture medium on shoot generation of Gerbera jamesonii by receptacle transverse thin cell layer culture

Nhựt, Dương Tấn; An, Truong Thi Thuy; Hương, Nguyễn Thị; Don, Nguyen Trinh; Hải, Nguyễn Thanh; Thien, Nguyen Quoc; Vu, Nguyen Hong

doi:10.1016/j.scienta.2006.10.008

Cited by 33 publications

(21 citation statements)

References 19 publications

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“…In all the varieties 7 -9 days old flower buds were more responsive (80 -90%) in callus induction and shoot proliferation (Table 1). There are some previous reports on using of flower bud as explants for shoot regeneration in gerbera (Pierik et al 1975, Laliberte et al 1985, Aswath and Choudhary 2002, Tyagi and Kothari 2004, Ray et al 2005, Kumar and Kanwar 2006and Nhut et al 2007. In the present study, flower stalk was also appeared to be a good responsive explant towards callus formation and shoot regeneration.…”

Section: Resultssupporting

confidence: 70%

In vitro Propagation in Three Varieties of Gerbera (Gerbera jamesonii Bolus.) from Flower Bud and Flower Stalk Explants

Akter

2013

Plant Tissue Cult. & Biotech.

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Three types of tender leaves derived explants such as leaf tip, leaf with mid-rib and leaf blade segments as well as flower buds and flower stalks obtained from three selected varieties of gerbera (Gerbera jamesonii Bolus.) cultivated in Bangladesh were exploited for callus induction and in vitro regeneration of plantlets. Among the explants flower bud and flower stalk were suitable and superior for callus induction and subsequent regeneration of in vitro shoots when cultured on MS supplemented with 5.0 mg/l BAP and 1.0 mg/l NAA. However, highest number of multiple shoots were obtained when the flower bud derived callus was subcultured on MS supplemented with 2.0 mg/l BAP. Variety with red petal showed the best response in producing multiple shoots among the three varieties. Root induction at the base of the in vitro regenerated shoots was tried using full and half the strengths of MS containing different concentrations of IBA. Best root induction was observed on half the strength of MS supplemented with 0.2 mg/l IBA. Following adequate acclimatization the regenerated plantlets were successfully transferred to soil where they grew to maturity and produced flowers. DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14203 Plant Tissue Cult. & Biotech. 22(2): 143-152, 2012 (December)

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Section: Resultssupporting

confidence: 70%

In vitro Propagation in Three Varieties of Gerbera (Gerbera jamesonii Bolus.) from Flower Bud and Flower Stalk Explants

Akter

2013

Plant Tissue Cult. & Biotech.

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“…Explant size may correlate to age, because the largest internodes (7 to 9 mm) were mostly obtained from the mid to bottom sections of the stem. Nhut et al (2007) reported that as the bud age of gerbera increased, the regeneration capability significantly increased. The researchers cited that not only was the physiological state different between the buds, but the size probably played some role because the larger tissues had more nutrient reserves, which can promote more shoot development (Nhut et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Plant Regeneration of Periwinkle (Catharanthus roseus) via Organogenesis

Swanberg¹,

Dai²

2008

horts

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Two periwinkle cultivars, Pacific Coral (P1) and Sunstorm Rose (P2), were used for development of a plant regeneration system. Leaf and internodal explants collected from in vitro plants were plated onto woody plant medium (WPM) using a factorial arrangement of 6-benzyladeine (BA) and 1-naphthalene acetic acid (NAA). Shoots were successfully regenerated. Shoot production from leaf tissues was minimal for all cultivars, whereas internodal tissues showed variable rates of regeneration depending on the hormone combination. Cultivar P1 showed the maximum regeneration rate (73.3%) when internodal explants, 4 to 6 mm in length, were placed on WPM containing 5 μm BA and 5 μm NAA. Cultivar P2 showed a regeneration rate of 56.7% with a combination of 20 μm BA and 10 μm NAA. Shoot regeneration rate increased as the internodal explant size increased for P2; however, the regeneration rate decreased when the explant size was greater than 7 mm for P1. The shoot regeneration rate decreased as the period of the dark treatment of internodal explants increased in both P1 and P2. The antibiotics carbenicillin (Carb) and cefotaxime (Cef) had little effect on shoot regeneration. There was a slightly higher rate observed for P1 when Cef was added into the medium, whereas P2 showed a decrease with the addition of Cef. Carb showed no significant effect on shoot regeneration for both cultivars. Addition of both Carb and Cef to the medium slightly inhibited shoot regeneration.

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“…For example, genotype proved to be a key factor influencing in vitro gynogenesis and androgenesis in summer squash (Cucurbita pepo), cucumber (Cucumis sativus), winter squash (Cucurbita maxima), pumpkin (Cucurbita moschata), and watermelon (Citrullus lanatus) 13,[15][16][17] . A previous study of Gerbera jamesonii including ten different Gerbera genotypes showed that genotype affected callus induction, which ranged from 5% to 43%, as well as the shoot regeneration rate, which ranged from 57% to 95% 18 . Several studies have also demonstrated that not all genotypes of G. jamesonii can produce haploid callus and shoots.…”

Section: Discussionmentioning

confidence: 97%

Haploid induction via unpollinated ovule culture in Gerbera hybrida

Fan

Cheng

Zhao

et al. 2020

Sci Rep

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Ovule-derived haploid culture is an effective and important method for genetic study and plant breeding. Gerbera hybrida is a highly heterozygous species, and the lack of homozygous lines presents a challenge for molecular genetic research. Therefore, we performed haploid induction through unpollinated ovule culture and evaluated the effects of several important factors on this culturing procedure in G. hybrida, including genotype, low temperature, and the development seasons of the ovules. Among 45 G. hybrida cultivars analyzed, 29 cultivars exhibited adventitious bud induction via in vitro unpollinated ovule culture with significant different responses, indicating that the genotype of donor plants was a vital factor for inducibility. Four cultivars with significantly different induction rates, including one non-induced cultivar, were selected to analyze seasonal effects. Ovules extracted in the summer consistently had the highest induction rates, and even the non-induced cultivar included in the analysis could be induced at low levels when ovules from summer were used. Low temperature treatment could also promote adventitious bud induction, and in particular, a strong and significant effect was detected after 7 days of cold treatment. Ploidy level measurements by flow cytometry revealed that 288 ovule-derived regenerants were haploid (55.17%) and 218 lines were diploid (41.76%). Moreover, genetic stability analysis of the regenerants indicated 100% similarity to the marker profile of the mother plant. This is the first report of ovule-derived haploids in G. hybrida, which may facilitate the development of homozygous lines for molecular research and plant breeding. Gerbera hybrida is one of the most popular ornamental plants worldwide 1. Since most of the commecial cultivars were bred from the crossing of wild-type G. jamesonii and G. viridifolia, the genome of G. hybrida is highly heterozygous 2. Hundreds of G. hybrida varieties with extremely rich flower color patterns are available in the flower market, including white, yellow, red, pink, purple, and brown, and G. hybrida ranks fourth in cut flowers after rose, chrysanthemum, and tulip 3. As a highly heterozygous species, G. hybrida naturally harbors genetic diversity, which is beneficial for hybridization breeding. However, the lack of a homozygous genome is challenging for molecular genetic research, including forward genetic screening and mutation mapping. Ploidy breeding, the modification of the number of chromosome sets in a plant genome, is frequently used in ornamental plant breeding to induce novel variation and to create homogenous lines 4. A commonly used protocol for ploidy breeding is haploid induction, which refers to haploid regeneration via a single gamete cell under specific conditions, using either pollen (anther or microspore culture) or egg cells (ovule culture) 5. Essentially, the applicability of haploid induction is the main trigger for ornamental breeding, and some haploids of ornamentals have been commercially developed, including the ...

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Effect of genotype, explant size, position, and culture medium on shoot generation of Gerbera jamesonii by receptacle transverse thin cell layer culture

Cited by 33 publications

References 19 publications

In vitro Propagation in Three Varieties of Gerbera (Gerbera jamesonii Bolus.) from Flower Bud and Flower Stalk Explants

In vitro Propagation in Three Varieties of Gerbera (Gerbera jamesonii Bolus.) from Flower Bud and Flower Stalk Explants

Plant Regeneration of Periwinkle (Catharanthus roseus) via Organogenesis

Haploid induction via unpollinated ovule culture in Gerbera hybrida

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