Effect of genotype and medium on wheat (Triticum aestivum L.) anther culture

Abstract

Four winter wheat (Triticum aestivum L.) and two spring wheat cultivars were evaluated in anther culture on three to four different media for their ability to initiate callus and green plants. Five media were used in the experiment: stored-potato medium with Ficoll 400, fresh-potato medium with Ficoll 400, fresh-t ~tato medium with agar, fresh-potato liquid medium without agar or Ficol1400, and a one tep 85D12-3 medium. Greatly different frequencies of calli and/or green plants were obtained from different cultivars and media. The callus initiation frequency varied from 2.7% for Arapahoe to 52% for Pavon, both on the stored potato medium with Ficoll 400. The frequency of green plant regeneration ranged from 0% for Arapahoe and Siouxland on the stored-potato medium with Ficoll 400 and 0% for Redland and Arapahoe in the fresh-potato medium with Ficoll 400 to 12% for Chris in the 85D12-3 medium (one-step procedure). Chris and Centurk 78, previously reported as having high levels of response, had significantly higher (P < 0.05) frequencies of green plant regeneration on the 85D12-3 medium than the othr-cultivars. An nexpected observation is that 'wet' MSC-medium enhanced callus regeneration mo:-. than a drier MSCmedium.

In vitro response of embryos from different provenances of Pinus caribaea var. hondurensis Morelet

“…Differences were observed in terms of shoot growth and production (Fazekas et al 1986;Juncker & Favre 1989), callus growth (Mikami & Kinoshita 1988;Myers et al 1989, Han-min et al 1990) and frequency of somatic embryogenesis (Chen et al 1987, Parrott et al 1989. Different genotypes of the same species respond differently to various culture media components such as coconut milk (Mathias & Simpson 1986), vitamins (Juncker & Favre 1989) and cytokinin concentration (Simpson & Bell 1989;Arnison et al 1990) and culture conditions such as cold pre-treatment (Powell 1988) and initial explant used (Chen et al 1987).…”

Wheat anther culture: effect of genotype and environmental conditions

“…However, there remain problems ranging from low frequency of callus induction to large genotypic and environmental effects. The response of anthers cultured on medium is dependent on the genotype of the donor plant [7,9,10,12], and the environmental conditions under which these plants are grown [4,6,9]. Here we report on the genotypic and environmental effects on anther culture of various cultivars and lines of common wheat, most previously unscreened.…”