Effect of Genotype and Explant Type on Shoot Regeneration in Tomato (Lycopersicon esculentum Mill.) in vitro

Gubiš, Jozef; Lajchová, Z.; Faragó, Juraj; Jureková, Zuzana

doi:10.17221/3715-cjgpb

Cited by 46 publications

(47 citation statements)

References 16 publications

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“…It was observed that BAP had positive effect towards multiple shoot regeneration, a result in agreement with that of the findings of Oktem et al (1999), Fariduddin et al (2004) and Sarker et al (2009). Addition of IAA was found to increase regeneration efficiency as reported earlier by Frary and Earle (1996), Gubis et al (2003) and Cortina and Culianez-Macia (2004).…”

Section: Discussionsupporting

confidence: 91%

Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth Regulators

Namitha

Negi

2013

Not Sci Biol

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A highly reproducible in vitro regeneration method for tomato (Lycopersicon esculentum Mill.) cultivar ' Arka Ahuti' was established by using hypocotyl, leaf and cotyledon explants from in vitro raised seedlings on Murashige and Skoog medium supplemented with different concentrations and combinations of hormones 6-Benzylamino purine (2 to 4 mg/L) and Indole-3-acetic acid (0.1 to 1 mg/L). The medium supplemented with 2 mg/L 6-benzylamino purine and 0.1 mg/L indole-3-acetic acid was found to be the best for inducing direct shoot regeneration and multiple shoots per explant from hypocotyl explants. Callus induction was observed in all the explants and regeneration of shoots was also promoted by all these combinations. Shoots were transferred to the elongation medium which also induced 100% rooting. After hardening, plants were transferred to soil. Thus, a tissue culture base line was established for ' Arka Ahuti' cultivar of tomato for obtaining direct regeneration using hypocotyl, leaf and cotyledon as explants.

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Section: Discussionsupporting

confidence: 91%

Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth Regulators

Namitha

Negi

2013

Not Sci Biol

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“…The frequency and response of in vitro shoot regeneration differed depending on the type of explants and both the type and concentration of growth regulators added to the regeneration medium (Gubiš et al 2003;Ishag et al 2009). Likewise, shoot bud induction from both CN and nodal explants was observed in all treatments containing TDZ though with varied frequencies.…”

Section: Effect Of Thidiazuronmentioning

confidence: 99%

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

Mukhtar

Ahmad²,

Khan³

et al. 2012

Physiol Mol Biol Plants

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A comparative performance of two explants types (CN and Nodal) for their efficiency to induce multiple shoot regeneration in Clitoria ternatea has been carried out. Thidiazuron (TDZ) in different concentrations (0.05-2.5 μM) was used as a supplement to the Murashige and Skoog's (MS) basal media. Explant type apart, two factors viz. concentration and exposure duration to TDZ played an important role in affecting multiple shoot regeneration. Cotyledonary node explants produced the best results at 0.1 μM TDZ, while in nodal explants the highest rate of shoot formation was achieved on MS medium supplemented with 1.0 μM TDZ. In both the explants, shoot multiplication increased when the regenerated shoots were subcultured on hormone free MS medium after 4 weeks of exposure to TDZ. Among the two, cotyledonary node explants produced considerably higher number of shoots at a comparatively lower concentration of TDZ than nodal explants. The regenerated shoots rooted best on MS medium containing 1.0 μM indole-3-butyric acid (IBA) and were successfully established in pots containing garden soil with 88 % survival rate. All the regenerated plants showed normal morphology and growth characteristics.

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“…Both we and MOGHAIEB et al (1999) Concluding remarks. This study confirmed our previous results that combining ZEA and IAA in the tissue culture medium had favourable effects on shoot organogenesis in tomato hypocotyl and cotyledon explants (GUBIŠ et al 2003). The transfer of hypocotyl explants onto a medium with half concentration of ZEA and IAA showed positive effects on shoot organogenesis.…”

supporting

confidence: 91%

Influence of growth regulators on plant regeneration in tomato

Gubiš¹,

Lajchová²,

Klčová³

et al. 2005

Hort. Sci. (Prague)

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ABSTRACT:We studied the effect of different plant growth regulators on in vitro regeneration and plant growth of three cultivars of tomato (Lycopersicon esculentum Mill.) from explants derived from hypocotyls and cotyledons of aseptically grown seedlings. The regeneration capacity was significantly influenced by cultivar and explant type. The highest number of shoots regenerated in both types of explants was recorded on MS medium supplemented with 1.0 mg/dm 3 zeatin and 0.1 mg/dm 3 IAA. The cultivar UC 82 showed the best regeneration capacity on all types of used media. The most responsive explants were hypocotyls with 90-92% regeneration in dependence on the used cultivars and mean production from 0.18 to 0.38 shoots per explant. 119of MURASHIGE and SKOOG (1962) (hereinafter abbreviated as MS), 100 mg/dm 3 myo-inositol, 2 mg/dm 3 thiamine.HCl, 0.5 mg/dm 3 pyridoxine. HCl, 0.5 mg/dm 3 nicotinic acid, 1% (w/v) sucrose and 0.7% (w/v) agar. The cultures were initially kept in the dark at 27 ± 1°C for two days and then maintained under a 16 h photoperiod at 50 µmol m 2 s, with day/night temperature of 25°C/20°C. Hypocotyl and cotyledon segments were cut from the seedlings grown in vitro. The hypocotyls were cut into three segments. Each cotyledon was transversally cut into two segments. Hypocotyls were transversally cut into 4-7 mm segments and leaf-blades into pieces of 30-40 mm 2 . The hypocotyl explants were placed horizontally on the medium surface and cotyledon explants with the adaxial surface in contact with the medium. Regeneration was induced on MS medium supplemented with different concentrations of cytokinins (ZEA, BAP, TDZ) and auxin (IAA) (Table 1). After 3 weeks cultured explants were transferred on: 1) MS without plant growth regulators (PGRs), 2) MS medium contains half concentration of PGRs and 3) MS medium with full concentration of PGRs (Fig. 1). The media were adjusted to pH 5.8 prior to autoclaving. Glass containers with 25 cm 3 of medium were used.Six weeks later the regeneration capacity of explants was assessed. The following parameters were evaluated: the frequency of regeneration (percent of regenerating explants) and the number of shoots per explant. Data on regeneration frequency (%) were transformed by arcsin√x prior to statistical analysis. The experiment was repeated twice using 30 explants per variant. Significance of difference between the results was estimated by analysis of variance (ANOVA). Variation among means was analysed using LSD (P ≤ 0.05) procedure. RESULTSThe effect of different tomato cultivars and different PGRs concentrations on shoot regeneration from aseptically grown hypocotyls and cotyledons showed significant variation in both the genotype and PGRs. A large variability in the number of shoots was observed between cultivars and between the different PGRs concentrations (Table 2). Cultivar mean comparisons (least significant difference, LSD, P ≤ 0.05) showed only two classes differing in the induction potential. The cultivar which produced the most shoots on hypocot...

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Effect of Genotype and Explant Type on Shoot Regeneration in Tomato (Lycopersicon esculentum Mill.) in vitro

Cited by 46 publications

References 16 publications

Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth Regulators

Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth Regulators

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

Influence of growth regulators on plant regeneration in tomato

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