Effect of genipin collagen crosslinking on porcine corneas

Ávila, Marcel Y.; Navia, José L.

doi:10.1016/j.jcrs.2009.11.003

Cited by 84 publications

(76 citation statements)

References 27 publications

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“…The use of proanthrocyanidin, glutaraldehyde, genipin, riboflavin, and carbodiimide has been proposed to enhance the mechanical and structural stability of dentin collagen, leading to a stable dentin matrix network that, after resin infiltration, should provide a durable hybrid layer (Al-Ammar et al, 2009;Macedo et al, 2009;Cova et al, 2011;Mazzoni et al, 2013b). In addition, collagen cross-linkers have been reported to improve the resistance of uncross-linked or mildly cross-linked collagen matrices to degradation by bacterial collagenases (Avila and Navia, 2010;Ma et al, 2010), potentially contributing to the stabilization of the resin-dentin interface over time.…”

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confidence: 99%

Carbodiimide Inactivation of MMPs and Effect on Dentin Bonding

et al. 2013

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The use of protein cross-linking agents during bonding procedures has been recently proposed to improve bond durability. This study aimed to use zymography and in situ zymography techniques to evaluate the ability of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) cross-linker to inhibit matrix metalloproteinase (MMP) activity. The hypotheses tested were that: (1) bonding procedures increase dentin gelatinolytic activity and (2) EDC pre-treatment prevents this enzymatic activity. The zymographic assay was performed on protein extracts obtained from dentin powder treated with Optibond FL or Scotchbond 1XT with or without 0.3M EDC pre-treatment. For in situ zymography, adhesive/dentin interfaces were created with the same adhesives applied to acid-etched dentin slabs pre-treated or not with EDC conditioner. Zymograms revealed increased expression of dentin endogenous MMP-2 and -9 after adhesive application, while the use of EDC as a primer inactivated dentin gelatinases. Results of in situ zymograpy showed that hybrid layers of tested adhesives exhibited intense collagenolytic activity, while almost no fluorescence signal was detected when specimens were pre-treated with EDC. The correlative analysis used in this study demonstrated that EDC could contribute to inactivate endogenous dentin MMPs within the hybrid layer created by etch-and-rinse adhesives.

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confidence: 99%

Carbodiimide Inactivation of MMPs and Effect on Dentin Bonding

et al. 2013

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“…Recently it has even been suggested as an efficient and safe treatment for macular detachment and retinoschisis in highly myopic eyes using a genipin cross-linked sclera as reinforcement [30]. Genipin is known for its excellent biocompatibility [31]. It is known to be less toxic than glutaraldehyde [32, 33], and as it does not need UV light activation, it will save the eye the toxic effects of UV light usage.…”

Section: Discussionmentioning

confidence: 99%

“…Soaking the cornea in genipin is definitely not a practical way to be used clinically, that is why using the drop method was our next step. Avila et al [31] mentioned that there was an evident increase in fiber density and a decrease in interfibrillar spaces in treated porcine corneas with topical 0.1, 0.25, and 1% genipin for 40 min. Without mentioning the percentages, they described these changes as dose dependent.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies on genipin for corneal CXL used different genipin concentrations (0.1, 0.25, 0.55, and 1%) with fixed treatment timing (5 min [31] or 40 min [15]). In our study we tried to find the appropriate duration for genipin treatment; we noticed that at setting concentration (0.5%) and varying the time, times greater than 15 min did not improve significantly the degree of CXL.…”

Section: Discussionmentioning

confidence: 99%

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Optimizing Genipin Concentration for Corneal Collagen Cross-Linking: An ex vivo Study

et al. 2018

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Purpose: Studying genipin variable concentrations, treatment durations, and delivery methods as a substance to increase corneal stiffness by inducing corneal collagen cross-linking (CXL). Materials and Methods: 100 bovine corneas treated with different genipin concentrations (0.1, 0.5, and 1%) and treatment durations (15 min, 40 min, 2 h, and 3 days) through different delivery methods compared to 10 controls treated with riboflavin/UV. Histology examination, enzymatic digestion with collagenase and thermal differential scanning calorimetry were performed on the different samples. Results: Bovine corneas soaked in 0.5% genipin morphologically showed 4.7% CXL in comparison to 5.6% in controls (p < 0.05). Corneas treated with topical 0.5% genipin, by a 140-µL drop applied hourly for 2 h, showed 7% corneal CXL. Corneas treated with topical genipin 0.5% for 30 min, 1 and 2 h showed 54 ± 6, 40 ± 7, and 39 ± 9% enzymatic degradation, respectively, in comparison to controls (74%). Corneas treated with 0.5% genipin for 1, 2, and 8 h showed higher thermal denaturation resistance (Td values of 64.9 ± 0.3, 64.7 ± 0.0 and 67.3 ± 0.9), respectively, in comparison to the control group (64.6 ± 0.5) (p < 0.05). Conclusions: Genipin 0.5%, in a 140-µL drop applied hourly for 2 h, showed better potential to enhance corneal stiffness and stability through inducing CXL.

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“…Genipin is a natural compound found in the fruit of Gardenia jasminoides. It has been tested on porcine corneas and found to significantly increase the stiffness of the corneas as well as increase resistance to collagenase (Avila and Navia 2010). In an animal study comparing genipin to riboflavin and UVA, rigidity was similar between the two and there was minimal toxicity to endothelial cells (Avila et al 2012).…”

Section: Other Methods Of Cross-linkingmentioning

confidence: 99%