Effect of GCSB-5, a Herbal Formulation, on Monosodium Iodoacetate-Induced Osteoarthritis in Rats

Kim, Joon-Ki; Park, Sang-Won; Kang, Jung‐Woo; Kim, Yu Jin; Lee, Sung Youl; Shin, Joon‐Shik; Lee, Sang-Ho; Lee, Sun-Mee

doi:10.1155/2012/730907

Cited by 49 publications

(47 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Proteoglycans consist of a protein core with GAG side chains. During inflammation and cartilage degradation, GAG monomers are released, which can be detected in synovial fluid and even in blood serum serving as the clinical parameter of OA [26]. Serum GAG levels were significantly increased in OAC group compared to HC ( p < 0.001) (Fig.…”

Section: Resultsmentioning

confidence: 99%

New herbal composition (OA-F2) protects cartilage degeneration in a rat model of collagenase induced osteoarthritis

Nirmal

Jagtap

Narkhede

et al. 2017

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundPrevalence of osteoarthritis (OA) is on rise on the global scale. At present there are no satisfactory pharmacological agents for treating OA. Our previous study showed that Sida cordifolia L. and Zingiber officinale Rosc. had protective effect on cartilage. Here, we describe the effect of OA-F2, a herbal formulation prepared using combination of these two plants in alleviating OA associated symptoms in a rat model of collagenase-induced OA.MethodsOA was induced by intra-articular injection of collagenase type II in wistar rats. Diclofenac (10 mg/kg) was used as a reference control. Rats (n = 6) were divided into 6 groups: Healthy control (HC), osteoarthritic control (OAC), diclofenac (DICLO), OA-F2L (135 mg/kg), OA-F2M (270 mg/kg) and OA-F2H (540 mg/kg). The effects of the 20 days treatment were monitored by parameters like knee diameter, paw volume, paw retraction; serum C-reactive protein (CRP), alkaline phosphatase (ALP) and glycosaminoglycan (GAG). Radiography and histopathology of knee joint were also studied. Additionally, gene expression was studied from isolated synovium tissue proving anti-osteoarthritic potential of OA-F2.ResultsOral administration of OA-F2 has significantly prevented knee swelling compared to OAC; OA-F2 and DICLO, significantly reduced paw volume compared to OAC. Paw latency was remarkably increased by OA-F2 compared to OAC. OA-F2L (−0.670, p < 0.001), M (−0.110, p < 0.05) and H (0.073) has markedly reduced levels of CRP compared to DICLO. OA-F2L (p < 0.05), M (p < 0.001) and H (p < 0.05) significantly reduced ALP levels, compared to DICLO. GAG release in the serum was also significantly lowered in OA-F2 treated group compared to DICLO. Radiological and histopathological observations showed cartilage protection by OA-F2. OA-F2 has upregulated SOD and GPx. Upregulated CAT expression was observed in OA-F2M and H. Considerable down-regulation of expression of MMP-3 and MMP-9 was observed in all the groups. Up-regulation of TIMP-1 was observed in rats treated with OA-F2L, H and DICLO.ConclusionOA-F2 has shown therapeutic effects in rat model of collagenase induced OA by demonstrating cartilage protection through controlling MMPs and improving anti-oxidant levels in arthritic synovium and is a potent candidate for further drug development and treatment for OA.

show abstract

Section: Resultsmentioning

confidence: 99%

New herbal composition (OA-F2) protects cartilage degeneration in a rat model of collagenase induced osteoarthritis

Nirmal

Jagtap

Narkhede

et al. 2017

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…Shinbaro1 and 2 solutions contain the herbal compound GCSB-5, derived from Chungpa-jun. The effects of GCSB-5 in anti-inflammation, neuroprotection, and articular cartilage protection have been reported in various experimental studies [20–22], and Shinbaro pharmacopuncture has similarly been proven to be effective for IDD [14, 23] and arthritis [24] through clinical research which explains the frequent use in M51 and M17 patients. The safety of Shinbaro pharmacopuncture has been demonstrated in single intramuscular dose toxicity tests [25] and repeated intramuscular dose toxicity tests [26].…”

Section: Discussionmentioning

confidence: 99%

Usage report of pharmacopuncture in musculoskeletal patients visiting Korean medicine hospitals and clinics in Korea

Lee

Shin

Lee

et al. 2016

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundPharmacopuncture is a relatively new acupuncture therapy combining acupuncture with herbal medicine. While pharmacopuncture is applied extensively in Korean medicine treatment, there are no clinical reports regarding what types of pharmacopuncture are used for which diseases.MethodsData was extracted retrospectively from the electronic medical records of all inpatients and outpatients at 12 Korean medicine hospitals and clinics during the period of December 17, 2010 to October 2, 2014. Treatment patterns for acupuncture, electroacupuncture and pharmacopuncture were analyzed. Principle diagnosis codes, frequency of treatment, pharmacopuncture type and costs were investigated to assess pharmacopuncture use in clinical settings.ResultsDuring the study period, a total 33,415 inpatients and 373,755 outpatients visited the study sites, and most were musculoskeletal. Among inpatients and outpatients, 98.6 % and 77.6 % received pharmacopuncture, respectively. Administration rate of pharmacopuncture for the 10 most frequent principle diagnosis codes was 97.2–99.3 % in inpatients, and that for outpatients was 73.0–91.5 %. The average number of pharmacopuncture sessions in pharmacopuncture recipients was 8.2 ± 12.3 for outpatients and 25.8 ± 18.7 for inpatients. The mean total cost for pharmacopuncture per patient was $556.24 ± 174.62 among inpatients, and $149.16 ± 243.85 among outpatients. Estimated average cost per pharmacopuncture session was $23–24 for inpatients, and $17–18 for outpatients. Shinbaro1, bee venom, Hwangryunhaedok, and Shinbaro2 were the most frequently used pharmacopuncture types.ConclusionsThis is the first analysis of treatment patterns of pharmacopuncture in a large-scale Korean medicine hospital/clinic patient population. We verified patterns of pharmacopuncture use for musculoskeletal disease treatment in Korea, and use of pharmacopuncture varied depending on disease or symptom severity. These results are expected to contribute to future clinical study design and standardization of pharmacopuncture.

show abstract

“…X-ray images have been widely used to detect loss of cartilage erosion, joint region, and osteophyte formation during the development of OA [62,69]. Thus, anti-OA agents should inhibit cartilage damage on X-ray images to indicate efficacy [49,70]. In our experiment, articular space narrowing, loss of the femoral and tibial AC erosion, joint region, and osteophyte formation were detected on roentgenographic images of the OA control rabbits.…”

Section: Discussionmentioning

confidence: 79%

Anti-Osteoarthritic Effects of a Mixture of Dried Pomegranate Concentrate Powder, Eucommiae Cortex, and Achyranthis Radix 5:4:1 (g/g) in a Surgically Induced Osteoarthritic Rabbit Model

Choi

Kang

Kim³

et al. 2020

Nutrients

View full text Add to dashboard Cite

In this study, we aimed to determine the synergistic effects of a formula consisting of dried pomegranate concentrate powder, Eucommiae Cortex, and Achyranthis Radix 5:4:1 (g/g) (PCP:EC:AR) in a surgically induced osteoarthritis (OA) rabbit model. PCP:EC:AR was orally administered once per day. Knee thickness, maximum extension of the knee joint, gross articular defect area, and the histopathological appearance of the cartilage were monitored, along with serum collagen type II C-telopeptide (CTX-II), cartilage oligomeric matrix protein (COMP), matrix metalloproteinase (MMP)-3, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and subchondral IL-1β and TNF-α levels. Roentgenographic images were also evaluated. PCP:EC:AR significantly inhibited the surgically induced increase in knee thickness, maximum extension of both knees, knee thickness after capsule exposure, gross femoral and tibial articular defect areas, loss of the knee joint area, serum and synovial COMP, CTX-II, and MMP expression, and synovial IL-1β, and TNF-α expression. In addition, surgically induced narrowing of the knee bones, loss of the joint area, cartilage damage, and osteophyte formation were reduced. PCP:EC:AR suppressed the surgically induced increases in the Mankin score, and subchondral IL-1β and TNF-α immunolabeled cell numbers. PCP:EC:AR exerted potent OA protective effects in a surgically induced OA rabbit model.

show abstract

Effect of GCSB-5, a Herbal Formulation, on Monosodium Iodoacetate-Induced Osteoarthritis in Rats

Cited by 49 publications

References 31 publications

New herbal composition (OA-F2) protects cartilage degeneration in a rat model of collagenase induced osteoarthritis

New herbal composition (OA-F2) protects cartilage degeneration in a rat model of collagenase induced osteoarthritis

Usage report of pharmacopuncture in musculoskeletal patients visiting Korean medicine hospitals and clinics in Korea

Anti-Osteoarthritic Effects of a Mixture of Dried Pomegranate Concentrate Powder, Eucommiae Cortex, and Achyranthis Radix 5:4:1 (g/g) in a Surgically Induced Osteoarthritic Rabbit Model

Contact Info

Product

Resources

About