Effect of gas—liquid interfaces on the growth of suspended mammalian cells: mechanisms of cell damage by bubbles

Handa-Corrigan, A.; Emery, A. N.; Spier, R.E.

doi:10.1016/0141-0229(89)90097-5

Cited by 241 publications

(90 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For cell lines with high oxygen requirements, 2 mm is suggested, meanwhile 5 mm is proposed for cells with low oxygen necessities (Freshney 2010). In our study, it was demonstrated that BHK-21 cell line can grow satisfactoryly in static cultures at 5 mm medium height, the maximum viable cell concentration were comparable with others previously reported using agitated systems (Handa-Corrigan et al 1989;Cruz et al 2002;Moreira et al 1995;Ishaque et al 2007). Increase of liquid column height is not recommend, this is based on unnecessary culture medium consumption (around 50 % for 10 mm liquid column height) and increasing cost of inoculation procedure.…”

Section: Discussionsupporting

confidence: 91%

“…The non-influence of inoculum volume-working volume percentage (10-30 %) on maximum viable cell concentration was useful for reducing handling, cell loss and contaminations risks associated to centrifugation operation in lab scale. Maximum viable cell concentration ([4 9 10 6 -cell mL -1 ) in optimal conditions was higher than usual values of this kinetic parameter for BHK-21 cell lines cultured in batch mode (1-3.2 9 10 6 cell mL -1 ) (Handa-Corrigan et al 1989;Cruz et al 2002;Moreira et al 1995;Ishaque et al 2007). Glutamine was exhausted at the end of the exponential growth phase (72 h post inoculation).…”

Section: Discussionmentioning

confidence: 79%

See 1 more Smart Citation

Approach toward an efficient inoculum preparation stage for suspension BHK-21 cell culture

et al. 2014

View full text Add to dashboard Cite

Mammalian cells are the most frequently used hosts for biopharmaceutical proteins manufacturing. Inoculum quality is a key element for establishing an efficient bioconversion process. The main objective in inoculation expansion process is to generate large volume of viable cells in the shortest time. The aim of this paper was to optimize the inoculum preparation stage of baby hamster kidney (BHK)-21 cells for suspension cultures in benchtop bioreactors, by means of a combination of static and agitated culture systems. Critical parameters for static (liquid column height: 5, 10, 15 mm) and agitated (working volume: 35, 50, 65 mL, inoculum volume percentage: 10, 30 % and agitation speed: 25, 60 rpm) cultures were study in T-flask and spinner flask, respectively. The optimal liquid column height was 5 mm for static culture. The maximum viable cell concentration in spinner flask cultures was reached with 50 mL working volume and the inoculum volume percentage was not significant in the range under study (10-30 %) at 25 rpm agitation. Agitation speed at 60 rpm did not change the main kinetic parameters with respect to those observed for 25 rpm. These results allowed for a schedule to produce more than 4 9 10 9 BHK-21 cells from 4 9 10 6 cells in 13 day with 1,051 mL culture medium.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 79%

Approach toward an efficient inoculum preparation stage for suspension BHK-21 cell culture

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Bu ajanın ortama ilavesi %0.1-0.2 civarında olmalıdır [83], [84]. Pluronic F 68'in ortama eklenmesi hücrenin kabarcıklara tutunmasını ve sıvı yüzeyinde kabarcık patlamasının hücre ölümüne sebep olmasını engellemektedir.…”

Section: Havalandırma çEşitleri Ve Hava Püskürtücüler (Hava Dağıtıcılar)unclassified

Transfection of Spodoptera frugiperda Insect Cell and Production of Recombinant Protein

Aytekin¹

2015

Pamukkale J Eng Sci

View full text Add to dashboard Cite

“…The degree of cell damage has been linked to bubble size by Oh et al, 1992and J6bes et al, 1991, localized the lethal region in a bubble column reactor. They showed like Handa-Corrigan et al, 1989, that cell damage occurs mainly at the liquid surface and in the foam layer. The main mechanisms causing this damage are the rapid bubble bursts with relatively high energy release, the shearing of cells in draining liquid films between bubbles in the foam and the physical loss of cells.…”

Section: Introductionmentioning

confidence: 99%

Design of a bubble-swarm bioreactor for animal cell culture

Gudermann

Lütkemeyer

Lehmann

1994

Cell Culture Engineering IV

View full text Add to dashboard Cite

A stationary bubble-swarm has been used to aerate a mammalian cell culture bioreactor with an extremely low gas flow rate. Prolonging the residence time of the gas bubbles within the medium improved the efficiency of the gas transfer into the liquid phase and suppressed foam formation. An appropriate field of speed gradients prevented the bubbles from rising to the surface. This aeration method achieves an almost 90% transfer of oxygen supplied by the bubbles. Consequently, it is able to supply cells with oxygen even at high cell densities, while sparging with a gas flow of only 0.22.10 -3 -1.45.10 -3 vvm (30-200 ml/h).The reactor design, the oxygen transfer rates and the high efficiency of the system are presented. Two repeated batch cultures of a rat-mouse hybridoma cell line are compared with a surface-aerated spinner culture. The used cell culture medium was serum-free, either with or without BSA and did not contain surfactants or other cell protecting agents. One batch is discussed in detail for oxygen supply, amino acid consumption and specific antibody production.

show abstract

Effect of gas—liquid interfaces on the growth of suspended mammalian cells: mechanisms of cell damage by bubbles

Cited by 241 publications

References 0 publications

Approach toward an efficient inoculum preparation stage for suspension BHK-21 cell culture

Approach toward an efficient inoculum preparation stage for suspension BHK-21 cell culture

Transfection of Spodoptera frugiperda Insect Cell and Production of Recombinant Protein

Design of a bubble-swarm bioreactor for animal cell culture

Contact Info

Product

Resources

About