Effect of Ganoderma lucidum on memory and learning in mice

Abstract

Background: In traditional Chinese medicine Ganoderma lucidum is famous for its beneficial effect on nervous system. It was traditionally used to manage different neurological disorders. This study was designed to investigate the nootropic potential of the mushroom. Methods: Nootropic activity was evaluated following the oral administration of two different doses (150 and 300 mg/kg) of ethanol extract of Ganoderma lucidum. Physostigmine (0.1 mg/kg) and Scopolamine (0.4 mg/kg) were used as positive and negative controls respectively. Distilled water was used as a control. Elevated plus maze and Morris water maze were used for the assessment of learning process. In elevated plus maze, initial transfer latency and retention transfer latency were assessed. In Morris water maze, TSTQ (time spent in target quadrant) and transfer latency were evaluated. For the assessment of locomotor activity, open field test was utilized. Furthermore, biochemical estimation of acetylcholinestrases (AchE) was performed by using Ellman's method. Results: The results of the study show that the Ganoderma lucidum extract has significantly increased the time spent in target quadrant (TSTQ) and decreased the escape latency (EL) in Morris water maze model. Whereas, a decrease in the Initial Transfer Latency (ITL) and Retention Transfer Latency (RTL) was observed in elevated plus maze model. Conclusion: The present study showed that Ganoderma lucidum has physostigmine like effect on brain acetylcholinesterase (AchE) activity.

Liquid-State Fermented Ganoderma lucidum GANO99 Regulates Gut Microbiota and Concomitantly Modulates the Behavioral Deficits and Neurohistopathological Hallmarks of Alzheimer’s Disease in a Preclinical Transgenic Mouse Model of Alzheimer’s Disease

“…Our study discovered that liquid-state fermented products of G. lucidum alleviated the impaired acquisition of spatial learning and memory. G. lucidum extract can increase spatial learning acquisition in the MWM test [23,24].…”