Effect of gamma irradiation on spleen cell function and cytotoxicity of doxorubicin

Lee, Ju-Woon; Sung, Nak-Yun; Kim, Jae‐Kyung; Kim, Jae‐Hun; Raghavendran, Hanumantha Rao Balaji; Yoo, Yung Choon; Mee-Hye, Shin; Byun, Myung‐Woo

doi:10.1016/j.cbi.2008.03.016

Cited by 10 publications

(5 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These functions are critical for normal conditions and even more so in diseased states such as cancer. Smaller spleen weight, as measured in this work (Table ), is consistent with other reports of reduced body weight and spleen size in ADR-treated mice ,− and supports the notion that altered cell differentiation as well as decreased lymphocyte proliferation , occurs with ADR treatment. Reduced spleen size is also reflective of lower numbers of lymphocytes, which occurs with aging, and could result in a weakened immune response in ADR-treated patients.…”

Section: Discussionsupporting

confidence: 92%

Global Effects of Adriamycin Treatment on Mouse Splenic Protein Levels

et al. 2012

View full text Add to dashboard Cite

Adriamycin (ADR) is a potent anticancer drug used to treat a variety of cancers. Patients treated with ADR have experienced side effects such as heart failure, cardiomyopathy, and "chemobrain", which have been correlated to changes in protein expression in the heart and brain. In order to better understand cellular responses that are disrupted following ADR treatment in immune tissues, this work focuses on spleen. Significantly reduced spleen sizes were found in ADR-treated mice. Global isotopic labeling of tryptic peptides and nanoflow reversed-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) were employed to determine differences in the relative abundances of proteins from ADR-treated mice relative to controls. Fifty-nine proteins of the 388 unique proteins identified showed statistically significant differences in expression levels following acute ADR treatment. Differentially expressed proteins are involved in processes such as cytoskeletal structural integrity, cellular signaling and transport, transcription and translation, immune response, and Ca(2+) binding. These are the first studies to provide insight to the downstream effects of ADR treatment in a peripheral immune organ such as spleen using proteomics.

show abstract

Section: Discussionsupporting

confidence: 92%

Global Effects of Adriamycin Treatment on Mouse Splenic Protein Levels

et al. 2012

View full text Add to dashboard Cite

show abstract

“…IL-2 is secreted by the Th1 subset and is essential for T cell activation and proliferation. Radiation can compromise T cell-mediated immunity by severely decreasing production of IL-2 (Han et al 2006, Attar et al 2007, Lee et al 2008. IL-4 is secreted by the Th2 cells, as well as mast and bone marrow stromal cells, and induces differentiation of naive T cells (Th0) into the Th2 subset.…”

Section: Introductionmentioning

confidence: 99%

Low-dose γ-rays modify CD4⁺T cell signalling response to simulated solar particle event protons in a mouse model

Rizvi¹,

Pecaut²,

Slater³

et al. 2010

International Journal of Radiation Biology

View full text Add to dashboard Cite

show abstract

“…Structure and purity were confirmed using several techniques, including melting point, NMR, MS, and elemental analysis. Each target compound was tested on human prostate (DU‐145) and breast (MCF‐7) cancer cell lines and compared with a reference compound, doxorubicin, a clinically used anticancer compound for breast and prostate cancers (20,21), using MTT assay. In this assay, the number of surviving cells is directly proportional to the level of formazan product formed because of reduction by mitochondrial dehydrogenases in living cells.…”

Section: Resultsmentioning

confidence: 99%

Syntheses and In Vitro Anticancer Properties of Novel Radiosensitizers

Ateş‐Alagöz¹,

Coleman²,

Martin³

et al. 2012

Chem Biol Drug Des

View full text Add to dashboard Cite

Series of 4-(ethylsulfonyl)-1-halogen-2-nitrobenzene (3a-e) and 1-(4-halogen-3-nitrophenyl) propan-1-one (5a-d) analogs designed as novel radiosensitizers using bromonitropropiophenone and bromonitrobenzonitrile as lead compounds were synthesized. The anticancer activities of the compounds were evaluated in vitro using human prostate cancer (DU-145) and breast cancer (MCF-7) cell lines and the MTT assay. From the series, six compounds (3b-e, 5b-c) exhibited potent growth inhibitory effects against both cell lines. The most active, compound 3d, is an iodosulfone and is significantly more potent than the lead compound 5c at 10 lM. Compounds were then compared with doxorubicin, a clinically used anticancer compound for breast and prostate cancers. Our most active compound 3d is more effective than doxorubicin at the dose level of 10 lM at 3 days after radiation, cell viabilities of 18%, 13% compared to 87%, 94% against MCF-7, and 15%, 20% compared to 60%, 75% against DU-145 without and with radiation, respectively. At 10 lM, compound 5c had no effects as compared to control, whereas compound 3d reduced DU-145 cell viability to 16% and that of MCF-7 cells to 9% even at 5 days after radiation. These results are very encouraging. Future studies include testing the compounds in vivo with and without radiation.

show abstract

Effect of gamma irradiation on spleen cell function and cytotoxicity of doxorubicin

Cited by 10 publications

References 29 publications

Global Effects of Adriamycin Treatment on Mouse Splenic Protein Levels

Global Effects of Adriamycin Treatment on Mouse Splenic Protein Levels

Low-dose γ-rays modify CD4⁺T cell signalling response to simulated solar particle event protons in a mouse model

Syntheses and In Vitro Anticancer Properties of Novel Radiosensitizers

Contact Info

Product

Resources

About

Effect of gamma irradiation on spleen cell function and cytotoxicity of doxorubicin

Cited by 10 publications

References 29 publications

Global Effects of Adriamycin Treatment on Mouse Splenic Protein Levels

Global Effects of Adriamycin Treatment on Mouse Splenic Protein Levels

Low-dose γ-rays modify CD4+T cell signalling response to simulated solar particle event protons in a mouse model

Syntheses and In Vitro Anticancer Properties of Novel Radiosensitizers

Contact Info

Product

Resources

About

Low-dose γ-rays modify CD4⁺T cell signalling response to simulated solar particle event protons in a mouse model