Effect of Galium verum aqueous extract on growth, motility and gene expression in drug-sensitive and -resistant laryngeal carcinoma cell lines

Schmidt, Marianne; Scholz, Claus‐Jürgen; Gavril, Georgiana; Otto, Christina; Polednik, Christine; Roller, Jeanette; Hagen, Rudolf

doi:10.3892/ijo.2013.2220

Cited by 18 publications

(31 citation statements)

References 39 publications

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“…On sensible primary mucosal cells, showing no p-glycoprotein expression, however, Galium decoction proved to be less toxic than in the HLaC78 laryngeal cancer cell line. In general, high motility of tumour cells is frequently correlated with increased chemoresistance, as previously shown for the paclitaxel-resistant head and neck cancer cell line Hep2-Tax (20). In HLaC78 cells, high cellular motility is combined with a slow division rate and chemosensitivity.…”

Section: Discussionmentioning

confidence: 64%

“…Potential main agents in Galium extracts, used for the present and previous study are chlorogenic acid, Luteolin-7-O-glucoside and rutoside (20). Chlorogenic acid has been shown to be antimetastatic in vivo and in vitro in a variety of tumour systems (30)(31)(32).…”

Section: Discussionmentioning

confidence: 99%

“…One batch of frozen Galium extract was used for all experiments. Identification of the extract ingredients is presented elsewhere (20).…”

Section: Methodsmentioning

confidence: 99%

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Galium verum aqueous extract strongly inhibits the motility of head and neck cancer cell lines and protects mucosal keratinocytes against toxic DNA damage

et al. 2014

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Galium verum, also known as Lady's Bedstraw, is an herbaceous plant native to Europe and Asia, and has been used in traditional medicine as an anticancer medicine applied in most cases as a decoction. The influence of a Galium verum decoction on the head and neck cancer cell lines HLaC78 and FADU was analyzed and proved to be toxic in high doses on both cell lines. Cytotoxicity appeared to be influenced by expression of p-glycoprotein (MDR-1) in the carcinoma cell lines. Mucosal keratinocytes, although void of MDR-1 expression, showed only low sensitivity against high Galium concentrations. Sublethal doses of Galium extract acted as strong inhibitors of motility, as shown by a spheroid-based invasion analysis on Matrigel-coated surfaces. Inhibition of invasion was significantly more pronounced in the invasive HLaC78 cell line. mRNA expression analysis of matrix metalloproteinases MMP-2 and MMP-9 and their inhibitors TIMP-1/-2 revealed significant TIMP-1 upregulation after an 8-h Galium exposition in FADU cells. Gelatinolytic activity, however, was not influenced by Galium extract in HLaC78, in the FADU cells MMP-2/-9 activity was slightly increased after incubation with Galium extract. In primary mucosal keratinocytes, Galium decoction protected DNA against benz[a]pyrene, one of the most DNA toxic agents in cigarette smoke. In conclusion Galium extract may be useful as a preventive and/or a concomitant therapeutic approach in head and neck cancer.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

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Galium verum aqueous extract strongly inhibits the motility of head and neck cancer cell lines and protects mucosal keratinocytes against toxic DNA damage

et al. 2014

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“…5 ). HLaC79-Tax is a descendant cell line of HLaC79 with acquired resistance against paclitaxel and corresponding over-expression of p-glycoprotein [ 17 ]. Here, the EC 50 dose of O. regalis extract was higher in HLaC79-Tax (20.6 μg/ml) compared to the parental cell line HLaC79 (9.9 μg/ml).…”

Section: Resultsmentioning

confidence: 99%

The influence of Osmunda regalis root extract on head and neck cancer cell proliferation, invasion and gene expression

Schmidt

Skaf

Gavril

et al. 2017

BMC Complement Altern Med

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BackgroundAccording to only a handful of historical sources, Osmunda regalis, the royal fern, has been used already in the middle age as an anti-cancer remedy. To examine this ancient cancer cure, an ethanolic extract of the roots was prepared and analysed in vitro on its effectiveness against head and neck cancer cell lines.MethodsProliferation inhibition was measured with the MTT assay. Invasion inhibition was tested in a spheroid-based 3-D migration assay on different extracellular matrix surfaces.Corresponding changes in gene expression were analysed by qRT-PCR array. Induction of apoptosis was measured by fluorescence activated cell sorting (FACS) with the Annexin V binding method. The plant extract was analysed by preliminary phytochemical tests, liquid chromatography/mass spectroscopy (LC-MS) and thin layer chromatography (TLC). Anti-angiogenetic activity was determined by the tube formation assay.Results O. regalis extract revealed a growth inhibiting effect on the head and neck carcinoma cell lines HLaC78 and FaDu. The toxic effect seems to be partially modulated by p-glycoprotein, as the MDR-1 expressing HLaC79-Tax cells were less sensitive. O. regalis extract inhibited the invasion of cell lines on diverse extracellular matrix substrates significantly. Especially the dispersion of the highly motile cell line HlaC78 on laminin was almost completely abrogated.Motility inhibition on laminin was accompanied by differential gene regulation of a variety of genes involved in cell adhesion and metastasis. Furthermore, O. regalis extract triggered apoptosis in HNSCC cell lines and inhibited tube formation of endothelial cells. Preliminary phytochemical analysis proved the presence of tannins, glycosides, steroids and saponins. Liquid chromatography/mass spectroscopy (LC-MS) revealed a major peak of an unknown substance with a molecular mass of 864.15 Da, comprising about 50% of the total extract. Thin layer chromatography identified ferulic acid to be present in the extract.ConclusionThe presented results justify the use of royal fern extracts as an anti-cancer remedy in history and imply a further analysis of ingredients.

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“…Other studies showing THM compounds displaying antiresistance impacts were also reported. For example, Galium verum aqueous extract profoundly suppressed cell invasion in Taxol-resistant human laryngeal carcinoma [55]. Danshen extract significantly inhibited proliferation of etoposide-and Taxol-resistant human oral cancer cells [112].…”

Section: Thm Extracts Modulate Therapeutic Sensitivity In Hnsccsmentioning

confidence: 99%

Traditional Herbal Medicine Mediated Regulations during Head and Neck Carcinogenesis

Lan¹,

Chung

Huang

et al. 2020

Biomolecules

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Head and neck squamous cell carcinoma (HNSCC) is one of the most prevalent neoplasms worldwide. It is well recognized that environmental challenges such as smoking, viral infection and alcohol consumption are key factors underlying HNSCC pathogenesis. Other than major clinical interventions (e.g., surgical resection, chemical and radiotherapy) that have been routinely practiced over years, adjuvant anticancer agents from Traditional Herbal Medicine (THM) are proposed, either alone or together with conventional therapies, to be experimentally effective for improving treatment efficacy in different cancers including HNSCCs. At a cellular and molecular basis, THM extracts could modulate different malignant indices via distinct signaling pathways and provide better control in HNSCC malignancy and its clinical complications such as radiotherapy-induced xerostomia/oral mucositis. In this article, we aim to systemically review the impacts of THM in regulating HNSCC tumorous identities and its potential perspective for clinical use.

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Effect of Galium verum aqueous extract on growth, motility and gene expression in drug-sensitive and -resistant laryngeal carcinoma cell lines

Cited by 18 publications

References 39 publications

Galium verum aqueous extract strongly inhibits the motility of head and neck cancer cell lines and protects mucosal keratinocytes against toxic DNA damage

Galium verum aqueous extract strongly inhibits the motility of head and neck cancer cell lines and protects mucosal keratinocytes against toxic DNA damage

The influence of Osmunda regalis root extract on head and neck cancer cell proliferation, invasion and gene expression

Traditional Herbal Medicine Mediated Regulations during Head and Neck Carcinogenesis

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