Effect of g.2728G&gt;A and g.3996T&gt;C Polymorphisms at the Leptin Gene <i>Locus</i> on Microstructure and Physicochemical Properties of <i>longissimus lumborum</i> Muscle of Polish Landrace Pigs

Wojtysiak, Dorota; Kaczor, Urszula

doi:10.3409/fb59_1-2.77-82

Cited by 13 publications

(4 citation statements)

References 19 publications

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“…Next, samples were cut on a cryostat (Slee MEV, Mainz, Germany) at −20°C into 10 μm thick slides. For the determination of the muscle histology profile the combined method was used: detection of NADPH dehydrogenase (diaphorase) according to Pears method and immunohistochemical detection of myosin heavy chains (MHCs) with NCL‐MHC antibodies (Leica, Novocasta) and NovoLink Polymer Detection Systems (Zalesie Gorne, Poland) (Leica, Novocasta) (Wojtysiak & Kaczor ). The slides were analyzed in terms of the percentage of the various types of muscle fibers – I, IIA and IIB (STO: slow‐twitch‐oxidative; FTO: fast‐twitch‐oxidative; FTG: fast‐twitch‐glycolytic) and their diameter by using Nikon Eclipse 50i light microscope and image analysis MultiScanBase ver.…”

Section: Methodsmentioning

confidence: 99%

Transcriptomic gene profiling of porcine muscle tissue depending on histological properties

Ropka‐Molik

Bereta

Żukowski

et al. 2016

Animal Science Journal

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In pig, the histological profile of muscle tissue, especially the proportion of individual fiber types, is one of the main factors affecting meat quality properties. In the present research, RNA sequencing (RNA-seq) by using next generation sequencing method was applied to estimate the whole gene expression profile of Longissimus lumborum muscle of pigs (Large White breed) differing in the percentage of two fiber types (slow-twitch (type I) fibers and fast-twitch glycolytic (type IIB) fibers). The RNA-seq approach allowed us to identify 355 differentially expressed genes (DEGs) indicated as significant (false discovery rate-adjusted P < 0.05) using three types of software: DESeq2, edgeR and baySeq. Detected genes and pathways deregulated in muscle depending on tissue microstructure were associated with: metabolic processes - 158 genes; cellular processes - 122; biological regulation - 62; localization - 51; and 35 genes with developmental processes. The DEGs were included in: PI3K-Akt; FoxO and MAPK signaling pathways, regulation of actin cytoskeleton, lysine degradation and insulin signaling pathway as well as mTOR and Hippo signaling pathways. These results highlight the mainly metabolic pathways related to glucose metabolism and contraction processes of muscle cells. Detection of genes involved in variation of fiber-type distribution will be useful in understanding of the genetic factors affecting muscle structure, metabolic process and indirectly, meat quality traits.

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Section: Methodsmentioning

confidence: 99%

Transcriptomic gene profiling of porcine muscle tissue depending on histological properties

Ropka‐Molik

Bereta

Żukowski

et al. 2016

Animal Science Journal

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“…An excessively pale meat color is undesirable consumers and also indicates the PSE meat defect (Warriss and Brown, 1995;Joo et al, 1999;Gajewczyk et al, 2014). Polish native breeds color is darkerfrom 43.88 to 54.94 (Table 4), than that observed in Polish Landrace -56.52 by Kasprzyk et al (2015), and53.07-54.26 (Wojtysiak andKaczor, 2011). The darkest meat color -43.88 was recorded for the Zlotnicka White breed (Grześkowiak et al, 2009), with L* being similar to recorded by Marsico et al (2007) for wild boars -43.63.…”

Section: Initial Ph Values Given In Tablementioning

confidence: 87%

Quality and processability of meat in polish native pig – a review

Szulc,

Nowaczewski,

Skrzypczak

et al. 2024

Annals of Animal Science

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The primary aim of pig breeding efforts is to produce animals characterized by outstanding performance, including also fattening and slaughter performance traits. However, improved carcass leanness and reduced carcass fatness, especially intramuscular fat content, have led to a deterioration of pork quality and processability. Due to the growing frequency of meat defects and the limited potential use of such meat in the production of premium products this constitutes a considerable problem for the meat industry. The breeds superior in terms of meat quality are native pig breeds, such as those kept in Poland, i.e. Pulawska (Pul), Zlotnicka Spotted (ZS) and Zlotnicka White (ZW) pigs. The aim of this paper is to present a review of research results concerning quality and processability of meat produced by the Polish native pig breeds. The presented results indicate that pigs of local breeds exhibit a low level of performance traits (daily gain, carcass leaness, backfat thickness) while maintaining very good parameters of meat quality and processability (muscle composition and physical properties, fatty acids profile, sensory traits). Thus, they are used as a source material for premium products. In view of the small size of local populations and higher production costs in Poland it is a niche production. Therefore, the current aim of breeding indigenous breeds is both to maintain a safe population size and genetic diversity and to increase popularity of their meat and processed meat products among consumers. This objective is attained, among other things, thanks to efforts to reduce of cost on their production through optimization of environmental conditions and wider use of less expensive traditional feed formulations, which make it possible to fully utilize the potential of local pork resources.

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“…Tissue samples (frozen at −80°C) were cut on 10 μm thick slides using a cryostat (Slee MEV, Mainz, Germany) and then fibers were detected using NADPH dehydrogenase (diaphorase) method. Myosin heavy chains (MHCs) were detected immunohistochemically with NCL-MHC antibodies (Leica, Novocasta, Leider Lane, Buffalo Grove, IL, USA) and NovoLink Polymer Detection Systems (Zalesie Gorne, Poland) (Leica, Novocasta, USA) [ 21 ]. The obtained preparations were analyzed in terms of the percentage content of types of muscle fibers (I, IIA, IIB) and their diameter using the Nikon Eclipse 50i light microscope and MultiScan image analysis software (ver.…”

Section: Methodsmentioning

confidence: 99%

Screening for candidate genes related with histological microstructure, meat quality and carcass characteristic in pig based on RNA-seq data

Ropka‐Molik

Bereta

Żukowski

et al. 2018

Asian-Australas J Anim Sci

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ObjectiveThe aim of the present study was to identify genetic variants based on RNA-seq data, obtained via transcriptome sequencing of muscle tissue of pigs differing in muscle histological structure, and to verify the variants’ effect on histological microstructure and production traits in a larger pig population.MethodsRNA-seq data was used to identify the panel of single nucleotide polymorphisms (SNPs) significantly related with percentage and diameter of each fiber type (I, IIA, IIB). Detected polymorphisms were mapped to quantitative trait loci (QTLs) regions. Next, the association study was performed on 944 animals representing five breeds (Landrace, Large White, Pietrain, Duroc, and native Puławska breed) in order to evaluate the relationship of selected SNPs and histological characteristics, meat quality and carcasses traits.ResultsMapping of detected genetic variants to QTL regions showed that chromosome 14 was the most overrepresented with the identification of four QTLs related to percentage of fiber types I and IIA. The association study performed on a 293 longissimus muscle samples confirmed a significant positive effect of transforming acidic coiled-coil-containing protein 2 (TACC2) polymorphisms on fiber diameter, while SNP within forkhead box O1 (FOXO1) locus was associated with decrease of diameter of fiber types IIA and IIB. Moreover, subsequent general linear model analysis showed significant relationship of FOXO1, delta 4-desaturase, sphingolipid 1 (DEGS1), and troponin T2 (TNNT2) genes with loin ‘eye’ area, FOXO1 with loin weight, as well as FOXO1 and TACC2 with lean meat percentage. Furthermore, the intramuscular fat content was positively associated (p<0.01) with occurrence of polymorphisms within DEGS1, TNNT2 genes and negatively with occurrence of TACC2 polymorphism.ConclusionThis study’s results indicate that the SNP calling analysis based on RNA-seq data can be used to search candidate genes and establish the genetic basis of phenotypic traits. The presented results can be used for future studies evaluating the use of selected SNPs as genetic markers related to muscle histological profile and production traits in pig breeding.

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Effect of g.2728G>A and g.3996T>C Polymorphisms at the Leptin Gene Locus on Microstructure and Physicochemical Properties of longissimus lumborum Muscle of Polish Landrace Pigs

Cited by 13 publications

References 19 publications

Transcriptomic gene profiling of porcine muscle tissue depending on histological properties

Transcriptomic gene profiling of porcine muscle tissue depending on histological properties

Quality and processability of meat in polish native pig – a review

Screening for candidate genes related with histological microstructure, meat quality and carcass characteristic in pig based on RNA-seq data

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