Effect of furosemide on Na+ and K+ transport studied by microperfusion of the rat nephron

Morgan, T. O.; Tadokoro, M.; Martin, Daniel R.; Berliner, Robert W.

doi:10.1152/ajplegacy.1970.218.1.292

Cited by 67 publications

(23 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This observation could reflect inhibition of ROMKmediated K ϩ recycling/secretion in the TAL and/or ROMKmediated K ϩ secretion in principal cells of the CD. In support of the first mechanism, Hropot and colleagues (4) demonstrated using free-flow micropuncture techniques that inhibition of NKCC2 with furosemide induces a marked increase in the delivery of K ϩ (and Na ϩ ) to the distal nephron, suggesting K ϩ recycling/secretion by ROMK in the TAL is a significant source of urinary K ϩ loss during furosemide treatment (7). If ROMK recycling/secretion in the TAL contributes to K ϩ wasting during bumetanide treatment, as we suspect it does, then inhibition of ROMK in the TAL by compound A is expected to limit the kaliuresis induced by this drug.…”

Section: Discussionmentioning

confidence: 99%

ROMK inhibitor actions in the nephron probed with diuretics

Kharade

Flores

Lindsley

et al. 2016

American Journal of Physiology-Renal Physiology

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

ROMK inhibitor actions in the nephron probed with diuretics

Kharade

Flores

Lindsley

et al. 2016

American Journal of Physiology-Renal Physiology

View full text Add to dashboard Cite

show abstract

“…Furthermore, because this electrogenic mode of sodium transport was not inhibited, the observed effect on jv could reflect inhibition of nonelectrogenic (i.e., neutral) sodium transport. Others have also found a reduction in proximal reabsorption with furosemide (26,(45)(46)(47)(48)(49)(50)(51) inhibitory activity of furosemide might have been expected to reduce J, by 0.34 nl/mm-min. However, the total effect of 3 mM furosemide was to reduce J, by 1.5 …”

Section: Passive Modes Of Naci Reabsorptionmentioning

confidence: 97%

Effects of Anion-Transport Inhibitors on NaCl Reabsorption in the Rat Superficial Proximal Convoluted Tubule

Lucci¹,

Warnock²

1979

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T The effects of anion-transport inhibitors on volume reabsorption, and total CO2 concentrations were examined by in vivo microperfusion of superficial proximal convoluted tubules of rats. The luminal perfusion solution was a high-chloride, lowbicarbonate solution like that in the in vivo late proximal tubule. The anion-transport inhibitors were only added to the luminal perfusion solutions.In tubules perfused with the control high-chloride solution, the rate of volume reabsorption (J,) was 2.3±0.2 nl/mm min (n = 18), and the collected total CO2 concentration was 4.0±0.3 mM. Furosemide (3 mM) caused a marked reduction in volume reabsorption to 0.8±0.3 nl/mmmin (n = 20) and only a slight increase in the total CO2 concentration of collected samples of perfusate (7.8±0.5 mM). 0.8 mM acetazolamide caused a more pronounced rise in the collected total CO2 concentrations to 10.7±0.5 mM but only a slight fall in J, to 1.7±0.3 nl/mm-min (n = 19). Hence, we inferred that inhibition of carbonic anhydrase only partially accounted for the inhibition of J, by furosemide. 4-acetamido-4'-iso-thiocyanato-stilbene-2,2'-disulphonic acid (0.1 mM), a well-characterized inhibitor of erythrocyte anion exchange mechanisms, also reduced J, to 1.6±0.3 nl/mm min (n = 15) without changing the total CO2 concentrations of the collected perfusates (3.6±0.4 mM). The effect of 4-acetamidoA4'-iso-thiocyanato-stilbene-2,2'-disulphonicPortions of this work were presented at the 10th Annual

show abstract

“…The TF/P K+ ratio in the earliest portion of the distal tubule accessible to micropuncture undergoes little change with variations in tubular fluid flow rate unless, in addition, potassium reabsorption in the ascending limb of the loop of Henle is affected as well (2,8,11). For this reason, the relationship between the TF/P K+ ratio and the flow rate of distal tubular fluid can best be appreciated by study of the late distal tubule.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of the Relationship between the Flow Rate of Tubular Fluid and Potassium Transport in the Distal Tubule of the Rat

Kunau¹,

Webb²,

Borman³

1974

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T The flow rate of tubular fluid has been suggested as one of several factors which may influence potassium transport in the distal convoluted tubule of the kidney. In the present micropuncture studies, the relationship between the flow rate of distal tubular fluid and potassium transport was examined in four groups of rats. Three groups of rats (I, II, and IV) were fed normal rat chow before study whereas one group (III) was fed chow containing 10% KC1. Group II received 10-20 pg/kg per h of d-aldosterone throughout the study.Distal tubular potassium transport in groups I, II, and III was examined before and after an increase in the flow rate of distal tubular fluid as induced by the infusion of an isotonic saline-bicarbonate solution equivalent to 10% of body weight. In group IV, distal tubular potassium transport was examined before and after enhancement of the flow rate by the infusion of hypertonic (15%) mannitol.In all four groups, distal tubular potassium secretion increased as the flow rate of tubular fluid increased. The nature of the relationship between distal tubular potassium transport and tubular fluid flow rate, however, was influenced by the extent to which the tubular fluid to plasma potassium ratio in the late distal tubule varied as the flow rate increased. As the flow rate was increased this ratio decreased significantly and to a comparable extent in groups I and II. In groups III and IV, on the other hand, this ratio was essentially identical during hydropenia and after the increase in the flow rate of tubular fluid. As a result, the increment in the amount of potassium present at the late distal tubule, which occurred as the flow rate increased, was significantly greater in groups III and IV than in groups I and II. The contrast in the relationship between the This work was presented in part at the American Society of Nephrology, Washington, D. C., November, 1973. Received for publication 10 May 1974 and in revised form 16 August 1974.flow rate of distal tubular fluid and potassium transport which were observed, probably reflects differences in the net driving force for cell to lumen potassium movement. Seemingly, the net driving force for potassium movement was maintained, as the flow rate of tubular fluid increased, by chronic potassium loading in group III and by hypertonic mannitol infusion in group IV.In groups I and II, the net driving force for potassium movement decreased as the flow rate of tubular fluid increased. However, the net driving force did not decrease in proportion to the increase in flow rate since potassium secretion was increased by increments in flow rate in these groups as well. We conclude that our results are consistent with the view that the flow rate of tubular fluid is a factor which can affect distal tubular potassium transport. However, the nature of the relationship between the flow rate of tubular fluid and potassium transport appears to depend upon the degree to which the driving force for cell to lumen potassium movement changes as the flo...

show abstract

Effect of furosemide on Na+ and K+ transport studied by microperfusion of the rat nephron

Cited by 67 publications

References 0 publications

ROMK inhibitor actions in the nephron probed with diuretics

ROMK inhibitor actions in the nephron probed with diuretics

Effects of Anion-Transport Inhibitors on NaCl Reabsorption in the Rat Superficial Proximal Convoluted Tubule

Characteristics of the Relationship between the Flow Rate of Tubular Fluid and Potassium Transport in the Distal Tubule of the Rat

Contact Info

Product

Resources

About