Effect of Furin inhibitor on lung adenocarcinoma cell growth and metastasis

Ma, Yongchao; Fan, Wei; Rao, Shu-Mei; Li, Gao; Bei, Zhan-Yu; Xu, Songtao

doi:10.1186/1475-2867-14-43

Cited by 21 publications

(18 citation statements)

References 16 publications

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“…Skin cancer Transgenic mouse model 58,59 vivo tumour growth. 26,30 These observations indicate that furin expression is not only a potential marker, 27 but also a potential therapeutic target for lung carcinomas.…”

Section: Other Cancersmentioning

confidence: 88%

“…Elevated levels of furin in NSCLC cell lines likely relies on the activity of PTGS2 and the transcription factor E2F1, as interference with either one decreases furin activity . Functional studies showed that inhibition of furin activity, either through genetic silencing or through application of the inhibitors PDX or CMK, decreases processing of the insulin‐like growth factor 1 receptor (IGF1R), cell proliferation, in vitro migration and invasion abilities, as well as in vivo tumour growth . These observations indicate that furin expression is not only a potential marker, but also a potential therapeutic target for lung carcinomas.…”

Section: Furin Promotes Tumour Progressionmentioning

confidence: 98%

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The proprotein convertase furin in tumour progression

Jaaks

Bernasconi

2017

Intl Journal of Cancer

114

113

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Proprotein convertases are proteases that have been implicated in the activation of a wide variety of proteins. These proteins are generally synthesised as precursor proteins and require limited proteolysis for conversion into their mature bioactive counterparts. Many of these proteins, including metalloproteases, growth factors and their receptors or adhesion molecules, have been shown to facilitate tumour formation and progression. Hence, this review will focus on the proprotein convertase furin and its role in cancer. The expression of furin has been confirmed in a large spectrum of cancers such as head and neck squamous cell carcinoma, breast cancer and rhabdomyosarcoma. Functional studies modulating furin activity uncovered its importance for the processing of many cancer-related substrates and strongly indicate that high furin activity promotes the malignant phenotype of cancer cells. In this review, we summarise the expression and function of furin in different cancer types, discuss its role in processing cancer-related proproteins and give examples of potential therapeutic approaches that take advantage of the proteolytic activity of furin in cancer cells.

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Section: Other Cancersmentioning

confidence: 88%

Section: Furin Promotes Tumour Progressionmentioning

confidence: 98%

The proprotein convertase furin in tumour progression

Jaaks

Bernasconi

2017

Intl Journal of Cancer

114

113

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“…Then MTT reagent (20 μL, 1.5 mg/mL in PBS) was added to each well and incubated for 4 h. The supernatant was removed, and 200 μL of DMSO was added to dissolve the formazan crystals. Absorbance was measured with spectrophotometer at 490 nm wavelength 45 .…”

Section: Methodsmentioning

confidence: 99%

“…After treatment at 6-well plates for 24 h, as the previously described 45 , cells were stained with dye Hoechst 33342at 37 °C for 5 min and avoided from light. Then, the cells were washed with 1 × PBS and images were captured on a Nikon fluorescence microscope (Nikon, Japan).…”

Section: Methodsmentioning

confidence: 99%

Coptisine from Rhizoma Coptidis Suppresses HCT-116 Cells-related Tumor Growth in vitro and in vivo

Huang

Xiao

Lin

et al. 2017

Sci Rep

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Colorectal cancer is one of the most common causes of cancer-related death in humans. Coptisine (COP) is a natural alkaloid from Coptidis Rhizoma with unclear antitumor mechanism. Human colon cancer cells (HCT-116) and xenograft mice were used to systematically explore the anti-tumor activity of COP in this study. The results indicated that COP exhibited remarkably cytotoxic activities against the HCT-116 cells by inducing G1-phase cell cycle arrest and increasing apoptosis, and preferentially inhibited the survival pathway and induced the activation of caspase proteases family of HCT-116 cells. Experimental results on male BALB/c nude mice confirmed that orally administration of COP at high-dose (150 mg/kg) could suppress tumor growth, and may reduce cancer metastasis risk by inhibiting the RAS-ERK pathway in vivo. Taken together, the results suggested that COP may be potential as a novel anti-tumor candidate in the HCT-116 cells-related colon cancer, further studies are still needed to suggest COP for the further use.

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“…As previously described 51 52 , VOA-treated cells were stained with apoptosis-specific dye Hoechst 33342. After treatment at 6-well plates for 48 h, cells were washed with 1×PBS for 3 times.…”

Section: Methodsmentioning

confidence: 99%

Volatile Oil of Acori Graminei Rhizoma-Induced Apoptosis and Autophagy are dependent on p53 Status in Human Glioma Cells

Chen

Jiang

et al. 2016

Sci Rep

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Acori Graminei Rhizoma is well known for the beneficial effects on CNS disorders in traditional medicine. Though it is frequently prescribed in formulations for brain tumors, the anti-glioma effect has not been examined. We used volatile oil of Acori Graminei Rhizoma (VOA) and human glioblastoma multiforme (GBM) cells in this study. We found that VOA exhibited greater growth suppression in p53 wild-type cells than p53 mutant cells and very low effect on fibroblasts and human glial HEB cells. Apoptosis was triggered by VOA with a caspase-dependent way in p53 wild-type A172 cells, while a caspase-independent way in p53 mutant U251 cells. Meanwhile, both A172 and U251 cells treated by VOA displayed autophagic features. Furthermore, p53 decrease was observed along with VOA-induced apoptosis and autophagy in A172 cells. VOA-induced autophagy was mediated through a p53/AMPK/mTOR signaling pathway in A172 cells, while an mTOR-independent signaling pathway in U251 cells. Finally, blockage of autophagy potentiated the proapoptotic effect in both A172 and U251 cells, indicating a protective role of autophagy in VOA-induced cell death. Together, VOA exhibited anti-tumor activity in human GBM cells and induced apoptotic cell death and protective autophagy, which is cell type specific and dependent on p53 status.

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Effect of Furin inhibitor on lung adenocarcinoma cell growth and metastasis

Cited by 21 publications

References 16 publications

The proprotein convertase furin in tumour progression

The proprotein convertase furin in tumour progression

Coptisine from Rhizoma Coptidis Suppresses HCT-116 Cells-related Tumor Growth in vitro and in vivo

Volatile Oil of Acori Graminei Rhizoma-Induced Apoptosis and Autophagy are dependent on p53 Status in Human Glioma Cells

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