Effect of Fungicide (Maneb) on Antioxydant System and Carbon Assimilation in Leaves of Sorghum Plants

Omari, Redouane El; Mrid, Reda Ben; Amakran, Amina; Nhiri, Mohamed

doi:10.1134/s1021443718020103

Cited by 8 publications

(3 citation statements)

References 22 publications

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“…R3 by Assem et al [56] has been identified as one of the two salt-tolerant alleles reported in the study. Further, the fungicide Maneb is also known to induce GLYI activity in sorghum indicating a pro-active antioxidant machinery operating in plants under such conditions [57]. However, among the D-LDH genes, only SbDLDH-1, encoding a putative mitochondrial protein, was found to be induced in response to ABA and PEG treatment.…”

Section: Discussionmentioning

confidence: 99%

From methylglyoxal to pyruvate: a genome-wide study for the identification of glyoxalases and D-lactate dehydrogenases in Sorghum bicolor

et al. 2020

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Background: The glyoxalase pathway is evolutionarily conserved and involved in the glutathione-dependent detoxification of methylglyoxal (MG), a cytotoxic by-product of glycolysis. It acts via two metallo-enzymes, glyoxalase I (GLYI) and glyoxalase II (GLYII), to convert MG into D-lactate, which is further metabolized to pyruvate by D-lactate dehydrogenases (D-LDH). Since D-lactate formation occurs solely by the action of glyoxalase enzymes, its metabolism may be considered as the ultimate step of MG detoxification. By maintaining steady state levels of MG and other reactive dicarbonyl compounds, the glyoxalase pathway serves as an important line of defence against glycation and oxidative stress in living organisms. Therefore, considering the general role of glyoxalases in stress adaptation and the ability of Sorghum bicolor to withstand prolonged drought, the sorghum glyoxalase pathway warrants an in-depth investigation with regard to the presence, regulation and distribution of glyoxalase and D-LDH genes. Result: Through this study, we have identified 15 GLYI and 6 GLYII genes in sorghum. In addition, 4 D-LDH genes were also identified, forming the first ever report on genome-wide identification of any plant D-LDH family. Our in silico analysis indicates homology of putatively active SbGLYI, SbGLYII and SbDLDH proteins to several functionally characterised glyoxalases and D-LDHs from Arabidopsis and rice. Further, these three gene families exhibit development and tissue-specific variations in their expression patterns. Importantly, we could predict the distribution of putatively active SbGLYI, SbGLYII and SbDLDH proteins in at least four different sub-cellular compartments namely, cytoplasm, chloroplast, nucleus and mitochondria. Most of the members of the sorghum glyoxalase and D-LDH gene families are indeed found to be highly stress responsive.Conclusion: This study emphasizes the role of glyoxalases as well as that of D-LDH in the complete detoxification of MG in sorghum. In particular, we propose that D-LDH which metabolizes the specific end product of glyoxalases pathway is essential for complete MG detoxification. By proposing a cellular model for detoxification of MG via glyoxalase pathway in sorghum, we suggest that different sub-cellular organelles are actively involved in MG metabolism in plants.

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Section: Discussionmentioning

confidence: 99%

From methylglyoxal to pyruvate: a genome-wide study for the identification of glyoxalases and D-lactate dehydrogenases in Sorghum bicolor

et al. 2020

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“…The method described by El Omari et al [29] was used to measure the activity of phosphoenolpyruvate carboxylase (PEPC). Glutamine synthetase (GS) and Aspartate aminotransferase (AAT) activities were measured using methods outlined by Kchikich et al [30], and Glutamate dehydrogenase (GDH) activity was measured spectrophotometrically for 30 minutes at 340 nm, as described by Ben Mrid et al [31].…”

Section: Extraction and Assay Of Nitrogen And Carbon Activitiesmentioning

confidence: 99%

Saccorhiza polyschides Extract as Biostimulant for Reducing Salt Stress Effect in Common Bean Crops

Nhhala,

Latique,

Kchikich

et al. 2024

Preprint

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Salinity stress emerges as an important menace to global food security, particularly affecting susceptible crops such as common bean plants (Phaseolus vulgaris L.). The disruption of ion balances caused by salt stress withing plants cells reduces water and nutrient absorption, and hampers growth and productivity. Sacchorhiza polyschides, a brown macroalga rich in essential elements, and macrominerals, has drawn attention as a natural biostimulant, being a potential remedy to bolster crop resilience against salt stress. This study delves into the effect of water extracts of Sacchorhiza polyschides (SPE) across varying concentrations (1%, 2%, and 5%) in common bean plants under salt stress (68.4 mM NaCl) conditions. Two different methods of application were used: by spraying the aerial part of the plant or by irrigation together with the watering solution. The effects of both systems of application were studied on the aerial parts of the plants. SPE exhibited significant enhancements in morphological parameters, chlorophyll content, carbon-nitrogen activities, and antioxidant enzymatic system activities, alongside increasing osmolyte accumulation, compared to salt-stressed plants without biostimulation. Moreover, SPE reduced reactive oxygen species (ROS) levels caused by salt stress, mitigating lipid peroxidation. The concentrations of 2% and 5% SPE applied by spraying showed the most significant results in most of the parameters analyzed. By shedding light on the underlying science behind this biostimulant effect, this research underscores the potential of SPE to enhance crop resilience and augment food production in salt-challenged environments.

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“…To maintain the work of our bodies normal, a balance between free radicals and antioxidant substances is necessary to prevent oxidative stress (El Omari et al, 2018;Jain et al, 2020;Romaniuk et al, 2018). In addition, this latter is considered a cause of several illnesses such as cancer, in ammation … etc.…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition and Antioxidant Activity of the Ceratonia Siliqua L. Seeds Oil

Abbout

CHEBABE

Rehioui

et al. 2021

Preprint

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In this work, we are interested in the antioxidant activity of Ceratonia Siliqua L. seeds oil. After extraction of seeds oil by Soxhlet, we characterized by GC-MS and evaluated the antioxidant activity by DPPH• and ABTS+•. The obtained results show that the seeds oil of Ceratonia Siliqua L. contains various organic compounds, the majority of which are fatty acids. Furthermore, the determination of IC50 by the DPPH• and ABTS+• methods show that cyclohexane has good IC50 compared to hexane.

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Effect of Fungicide (Maneb) on Antioxydant System and Carbon Assimilation in Leaves of Sorghum Plants

Cited by 8 publications

References 22 publications

From methylglyoxal to pyruvate: a genome-wide study for the identification of glyoxalases and D-lactate dehydrogenases in Sorghum bicolor

From methylglyoxal to pyruvate: a genome-wide study for the identification of glyoxalases and D-lactate dehydrogenases in Sorghum bicolor

Saccorhiza polyschides Extract as Biostimulant for Reducing Salt Stress Effect in Common Bean Crops

Chemical Composition and Antioxidant Activity of the Ceratonia Siliqua L. Seeds Oil

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