Effect of fungal damage by Fusarium spp and Diaporthe/Phomopsis complex on protein quantity and quality of soybean seed

Abstract

Seeds from three soybean genotypes were tested to evaluate the effect of fungal damage by Fusarium spp and Diaporthe/Phomopsis complex on protein quantity and quality. Fungus-infected seeds had higher protein contents than uninfected ones. A selective degradation of soluble proteins was detected in seeds infected with either fungus. Some of the storage proteins degraded were identified as α , α and β subfractions of the β-conglycinin, and A 3 subfraction of the glycinin. Furthermore, reductions in lipoxygenase and trypsin inhibitor activities were observed in fungus-infected seeds. Amino acid composition did not vary between infected and uninfected seed lots, so protein degradation should not affect amino acid structures.

Soybean Seed Composition Constituents as Affected by Drought andPhomopsisin Phomopsis Susceptible and Resistant Genotypes

“…and Diaporthe/Phomopsis complex-infected soybean seeds. The increase of protein and oleic acid in S genotypes in MG V at harvest maturity and 15 days after harvest maturity in 2003 and 2005 under the non-irrigated environment in our results is supported by previous research (Wilson, Novitzky, & Fenner 1995;Begum et al 2008;Meriles et al 2004). However, our results in MG III or MG V under the irrigated Downloaded by [Anadolu University] at 07:11 20 December 2014 environment cannot be compared to the literature as the available literature is on the effect of Phomospsis on seed composition in inoculated and non-inoculated susceptible genotypes.…”

“…The increase in protein and oleic acid was also reported in corn, groundnut, and soybean seeds infected by different fungal species, including Aspergillus, Penicillium, Fusarium, Curvularia, Alternaria, and Rhizopus (Bhattacharya & Raha 2002). Meriles et al (2004) also detected a high level of oleic acid and lower levels of linoleic and linolenic acids in Fusarium spp. and Diaporthe/Phomopsis complex-infected soybean seeds.…”

“…Fungal-infected soybean seed had higher protein concentrations (Meriles et al 2004), lower carbohydrates, and no change or increased oil concentrations (Pathan, Sinclair, & McClary 1989.). Fungal infection by C. truncatum increased protein and oleic acid content and reduced linoleic acid content but did not change in extracted oil and other fatty acids when compared with un-inoculated seeds after four days of incubation (Begum et al 2008).…”

Caracterización morfológica y molecular de una colección de aislamientos de Phomopsis longicolla (teleomorfo desconocido: Diaporthales) de la región templada y subtropical de Argentina

“…sojae (Pps) (teleomorfo D. phaseolorum var. sojae) producen el Tizón del tallo y vainas y luego el Decaimiento o podredumbre de semillas en soja (TTVyS), afectan significativamente el número, el peso y la calidad de semillas y granos secos, como también los frutos y granos para consumo verde (Pioli, Benavídez, & Morandi, 1997;Pioli, Benavídez, Morandi, & Bodrero, 2000;Meriles, Lamarque, Labuckas, & Maestri, 2004;Benavídez, Pioli, & Morandi, 2010). Hobbs, Schmitthenner y Kuter (1985) realizaron una minuciosa descripción morfológica y fisiológica de estos Phomopsis, a los que denominaron P. longicolla, distinguién-dolos de aquellos previamente identificados como P. sojae sin.…”

Cytochalasins from the endophytic fungus Phomopsis sp. cib-109

“…is a widely distributed endophytic fungus, some of which can cause serious plant diseases such as leaf blight, twig dieback, fruit rot, and seed decay [1][2][3][4][5][6][7][8]. Cytochalasins were isolated from the genus Phomopsis as the main phytotoxic metabolites, which can inhibit the growth of the plants [9][10][11][12].…”