Effect of Functionalization of 2D Graphene Nanosheets on the Oxidation Stress the Cellular Response of BEAS-2B Cells

Abstract

The present study is intended to determine the physiochemical properties of functionalized graphene leading to potential molecular or cellular injury. We investigated the role of functionalization of graphene oxide nanoflakes (GONPs) and the cellular responses in terms of viability and cytotoxicity of BEAS-2B cells. GONPs were synthesized by hummer’s method and obtained fine GONPs of 1.87 nm size. The crystalline dimensions, microstrain, and dislocation densities of the GONPs were analyzed by using XRD and a FESEM was used to determine the shape of the as-synthesized GONPs. The cytotoxicity and oxidative stress were evaluated using cultured BEAS-2B cells. GONPs were exposed to the cultured cells in an incremental dosage pattern. The interactions of GONPs with the cultured cells were studied using the analytical techniques flow cytometry, and FE-SEM imaging. It is found that GONPs interacted with the plasma membrane and whereas high concentration GONPs were detached from the cells and remained as foreign bodies which is observed through FESEM analysis From the obtained results we summarize the effect of functionalization of GNPs and their altered properties and their mechanism of interaction with non-phagocytic cell lines.