Effect of Frequency of Sucrose Exposure on Dental Biofilm Composition and Enamel Demineralization in the Presence of Fluoride

Ccahuana-Vásquez, Renzo Alberto; Tabchoury, Cínthia Pereira Machado; Tenuta, Lívia Maria Andaló; Cury, Altair Antoninha Del Bel; Vale, Gláuber Campos; Cury, Jaime Aparecido

doi:10.1159/000096100

Cited by 105 publications

(111 citation statements)

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“…Caries lesions are found only when the teeth are subjected to the caries process several times per day for many days. 8 However, early caries lesions can be halted or even repaired, and fluoride is a potent activator of this process. 9 Similarly, regarding fluorosis, several days of exposure to high fluoride concentrations are necessary during enamel formation for visible changes to be clinically detected in the future.…”

Section: Introductionmentioning

confidence: 99%

Fluoride concentrations in the water of Maringá, Brazil, considering the benefit/risk balance of caries and fluorosis

et al. 2015

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Fluoride concentrations in the water of Maringá, Brazil, considering the benefit/risk balance of caries and fluorosis Abstract: Current Brazilian law regarding water fluoridation classification is dichotomous with respect to the risks of and benefits for oral diseases, and fluoride (F) concentrations less than 0.6 or above 0.8 mg F/L are considered outside the normal limits. Thus, the law does not consider that both caries and fluorosis are dependent on the dosage and duration of fluoride exposure because they are both chronic diseases. Therefore, this study evaluated the quality of water fluoridation in Maringá, PR, Brazil, considering a new classification for the concentration of F in water the supply, based on the anticaries benefit and risk of fluorosis (CECOL/USP, 2011). Water samples (n = 325) were collected monthly over one year from 28 distribution water networks: 20 from treatment plants and 8 from artesian wells. F concentrations were determined using a specific ion electrode. The average F concentration was 0.77 mg F/L (ppm F), ranging from 0.44 to 1.22 mg F/L. Considering all of the water samples analyzed, 83.7% of them presented from 0.55 to 0.84 mg F/L, and according to the new classification used, they would provide maximum anticaries benefit with a low risk of fluorosis. This percentage was lower (75.4%) in the water samples supplied from artesian wells than from those distributed by the treatment plant (86%). In conclusion, based on the new classification of water F concentrations, the quality of water fluoridation in Maringá is adequate and is within the range of the best balance between risk and benefit.

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Section: Introductionmentioning

confidence: 99%

Fluoride concentrations in the water of Maringá, Brazil, considering the benefit/risk balance of caries and fluorosis

et al. 2015

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“…Ten healthy volunteers were selected on the basis of the criteria defined in previous studies. 11,16 The in situ experiment involved a randomized double-blind crossover design performed in 3 phases with 20% sucrose solution (control treatment), fermented milk 1 (treatment A), and fermented milk 2 (treatment B). Each participant wore an acrylic palatal device.…”

Section: In Situ Experimentsmentioning

confidence: 99%

“…Insoluble extracellular polysaccharides (EPS) were extracted. 16,20 Carbohydrates were analyzed by using the phenol-sulfuric acid procedure. 12 The results are expressed as mg/g dry weight.…”

Section: Biofilm Analysismentioning

confidence: 99%

Effects of probiotic fermented milk on biofilms, oral microbiota, and enamel

et al. 2015

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The aim of this study was to evaluate in vitro and in vivo the effects of 2 brands of probiotic fermented milk on biofilms, oral microbiota, and enamel. For the in situ experiment, ten volunteers wore palatine devices containing four blocks of bovine dental enamel over 3 phases, during which 20% sucrose solution, Yakult ® (Treatment A), and Batavito ® (Treatment B) were dropped on the enamel blocks. Salivary microbial counts were obtained and biofilm samples were analyzed after each phase. For the in vivo experiment, the same ten volunteers drunk Yakult ® (Treatment C) and Batavito ® (Treatment D) in two phases. Saliva samples were collected for microbial analysis after each phase. The in situ study showed that in comparison with Treatment A, Treatment B resulted in fewer total cultivable anaerobes and facultative microorganisms in biofilms, higher final microhardness, lower percentage change in surface hardness, and smaller integrated subsurface enamel hardness. In the in vivo study, Treatment D resulted in a reduction in the counts of all microorganisms. The results suggested that the probiotic fermented milk Batavito ® , but not Yakult ® , reduced the amount of oral microorganisms and mineral loss in bovine enamel.

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“…The relationship between sugar exposure and fluoride effect on caries has been evaluated in well-controlled in situ studies (8)(9)(10)(11). Duggal et al (9) and Ccahuana-Vasquez et al (10) showed that if the daily frequency of sugar were not above 7 to 8 times/day, fluoride from 1,100 µg/g toothpaste would be effective to control enamel demineralization.…”

Section: Introductionmentioning

confidence: 99%

“…Duggal et al (9) and Ccahuana-Vasquez et al (10) showed that if the daily frequency of sugar were not above 7 to 8 times/day, fluoride from 1,100 µg/g toothpaste would be effective to control enamel demineralization. Cury et al (11) evaluated the relationship between fluoride concentration in toothpaste and sugar exposure on human enamel demineralization.…”

Section: Introductionmentioning

confidence: 99%

Effect of Fluoride Concentration on Reduction of Enamel Demineralization According to the Cariogenic Challenge

et al. 2016

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Fluoride present in toothpaste at 1,100 µg/g is considered effective on caries control. However, under high cariogenic challenge due to increasing sugar exposure, higher fluoride concentration (5,000 µg/g) could be necessary to compensate the unbalance on caries process. This was tested in a pH-cycling regimen, which evaluated the effect of fluoride concentration relative to toothpaste on reduction of enamel demineralization under conditions of two levels of cariogenic challenge. Enamel slabs (n=20) were subjected to two pH-cycling regimens, simulating 8x and 16x/day sugar exposure and were treated with solutions containing: 0 (no fluoride), 275 or 1,250 µg F/mL, resulting in 6 treatment groups: 4-h/0-F; 8-h/0-F; 4-h/275-F; 8-h/275-F; 4-h/1,250-F and 8-h/1,250-F. The 275 and 1,250 µg F/mL concentrations simulate mouth salivary dilution when 1,100 and 5,000 µg/g toothpastes are used. Enamel demineralization was assessed by surface (%SHL) and cross-sectional hardness. Fluoride taken up by enamel was also evaluated. Data were analyzed by ANOVA one-way and Tukey's test. The treatment with 1,250 µg F/mL significantly reduced %SHL compared with 275 µg F/mL (p<0.05), irrespective the level of cariogenic challenge (4-h/1,250-F vs. 4-h/275-F and 8-h/1,250-F vs 8-h/275-F comparisons, respectively). These data were supported by fluoride concentration found in enamel. These findings suggest that higher fluoride concentrations could partly compensate the greater caries risk under higher cariogenic challenge due to increasing sugar exposure.

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Effect of Frequency of Sucrose Exposure on Dental Biofilm Composition and Enamel Demineralization in the Presence of Fluoride

Cited by 105 publications

References 50 publications

Fluoride concentrations in the water of Maringá, Brazil, considering the benefit/risk balance of caries and fluorosis

Fluoride concentrations in the water of Maringá, Brazil, considering the benefit/risk balance of caries and fluorosis

Effects of probiotic fermented milk on biofilms, oral microbiota, and enamel

Effect of Fluoride Concentration on Reduction of Enamel Demineralization According to the Cariogenic Challenge

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