Effect of freezing–thawing protocols on the performance of semen from three rabbit lines after artificial insemination

Mocé, E.; Vicente, J.S.; Lavara, R.

doi:10.1016/s0093-691x(02)01329-8

Cited by 42 publications

(42 citation statements)

References 26 publications

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“…Other smallscale experiments using 1 and 2 M Me 2 SO (Hanada andNagase, 1980 andKashiwazaki et al, 2006, respectively) showed no differences between Me 2 SO and acetamide as cryoprotectants for rabbit sperm freezing. On the contrary, several authors have demonstrated the efficient sperm protection of 3.0 and 3.5 M Me 2 SO in rabbit sperm cryopreservation Viudes de Castro and Vicente, 1996;Mocé et al, 2003;Cortell and Viudes de Castro, 2008;Iaffaldano et al, 2012;Rosato and Iaffaldano, 2013). According to our results, when Me 2 SO was used v. acetamide extender, results on frozen-thawed sperm quality and reproductive parameters studied were improved.…”

Section: Discussionsupporting

confidence: 51%

“…Although TB was significantly higher for the LP line (6.1 v. 4.8), and, consistently, foetal and gestational losses were significantly lower in this case. In previous studies with frozen semen in rabbit, differences in fertility rate and litter size were reported when comparing paternal and maternal lines (Mocé et al, 2003), but no differences were observed between maternal lines. In the present study, line LP, selected by longevity and that had prolificacy near or above the population average, seems to sustain fertility and prolificacy of frozen semen better than H line, selected by hyperprolificacy.…”

Section: Discussionmentioning

confidence: 89%

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Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

Viudes-de-Castro

Lavara

Safaa

et al. 2014

Animal

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This study was conducted to elucidate the effect of different freezing extenders on two lines selected for hyperprolificacy and longevity (H and LP, respectively). In extender A, dimethyl sulphoxide (Me 2 SO) and sucrose were used as cryoprotectants. In extenders B and C, the sucrose was replaced by 20% egg yolk, and in extender C the Me 2 SO was substituted by acetamide. Semen was packaged in 0.25 ml plastic straws and cooled at 5°C for 45 min, and then was frozen in liquid nitrogen vapour for 10 min before being plunged into the liquid nitrogen. Thawing was carried out by immersing the straws in a water bath at 50°C for 10 s. Frozen-thawed semen characteristics and reproductive parameters were affected by freezing. Extender C showed significantly lower post-thawing quality traits than any of the three extenders. Acrosome integrity was significantly improved when Me 2 SO was used as cryoprotectant. Sucrose replacement by 20% egg yolk had no effect on acrosome integrity but provided significantly lower sperm motility and viability. Freezing extender affected fertility rate, total born, number of implantation sites and gestational losses, obtaining better results when extender A was used. The acrosomal integrity after frozen-thawed process showed a significant correlation with fertility at 12 th day and also at birth, indicating that an increase in acrosomal integrity leads to an increase in both fertilities (12 th day and at birth). A positive correlation between motility of semen and implantation sites was found. The post-thawing quality traits of semen were not affected by the genetic line, although LP line showed higher total born and lower foetal and gestational losses. The findings of this study suggest that freezing extender composition has a significant effect on the success of rabbit sperm for preservation, and when Me 2 SO was used as permeable cryoprotectant sucrose provided better protection compared with egg yolk and improved reproductive traits, and, on the other hand, the male genotypes used in the present study had no effect on frozen-thawed sperm parameters but negatively affected some of the reproductive parameters.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 89%

Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

Viudes-de-Castro

Lavara

Safaa

et al. 2014

Animal

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“…In early research on freezing of rabbit spermatozoa, spermatozoa frozen with glycerol resulted in extremely low fertility [8][9][10][11]. After 1980, studies employing acetamide [12][13][14][15] or DMSO [12,[16][17][18][19][20][21][22] as a cryoprotectant have been encouraging. H o w e v e r , t h e r e i s a l i t t l e i n f o r m a t i o n o n cryoprotectants when freezing Japanese white rabbit spermatozoa.…”

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confidence: 99%

Comparison of Glycerol, Lactamide, Acetamide and Dimethylsulfoxide as Cryoprotectants of Japanese White Rabbit Spermatozoa

et al. 2006

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Abstract. The rabbit is considered to be a valuable laboratory animal. We compared glycerol, lactamide, acetamide, and dimethylsulfoxide (DMSO) as cryoprotectants in egg-yolk diluent of ejaculated Japanese white rabbit spermatozoa for improvement of sperm cryopreservation methods. Rabbit semen was frozen with 1.0 M glycerol, lactamide, acetamide, or DMSO in plastic straws. Forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rate of forward progressive motile spermatozoa in lactamide (37.8 ± 3.0%) was significantly (P<0.05) higher than in glycerol (17.0 ± 3.3%). In addition, the rates of sperm plasma membrane integrity in lactamide and acetamide (35.9 ± 3.3% and 30.2 ± 3.0%, respectively) were significantly (P<0.05) higher than in glycerol (17.0 ± 2.6%). The results indicate that 1.0 M lactamide and acetamide have higher cryoprotective effects than 1.0 M glycerol for cryopreservation of Japanese white rabbit spermatozoa. Key words: Acetamide, Cryopreservation, Glycerol, Lactamide, Rabbit spermatozoa (J. Reprod. Dev. 52: [511][512][513][514][515][516] 2006) he rabbit is considered to be a valuable laboratory animal for research in teratology, immunology, microbiology, and medical and life sciences. In addition, many valuable mutants [1,2] including transgenic [3][4][5][6], have been established in the rabbit. Therefore, a need has been recognized for reliable methods to bank rabbit genetic resources efficiently in the form of cryopreserved spermatozoa. Glycerol has been extensively used as a cryoprotectant for various cells, including mammalian spermatozoa and embryos, since Polge et al. [7] reported its cryoprotective properties. In early research on freezing of rabbit spermatozoa, spermatozoa frozen with glycerol resulted in extremely low fertility [8][9][10][11]. After 1980, studies employing acetamide [12][13][14][15] or DMSO [12,[16][17][18][19][20][21][22] as a cryoprotectant have been encouraging. H o w e v e r , t h e r e i s a l i t t l e i n f o r m a t i o n o n cryoprotectants when freezing Japanese white rabbit spermatozoa.In the present study, we compared 1.0 M glycerol, lactamide, acetamide, and DMSO as cryoprotectants in egg-yolk diluent for ejaculated spermatozoa of the Japanese white rabbit. Forward progressive motility and plasma membrane

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“…Hücre membranlarında oluşan perforasyonların, özellikle akrozomda bulunan ve fertilizasyonda görev alan enzimlerin kaybına yol açtığı ve bu spermatozoanın döllemeyi yapamadığı kabul edilmektedir (21). Değişik ve çeşitli oranlarda kryoprotektanları içeren sperma sulandırıcılarının sözü edilen dondurma aşamalarında spermatozoayı soğuk ekisinden korumaları, bulundukları ortamdaki madde alışverişini ayarlamaları ve membranları çevresinde oluşan buz kristalizasyonlarının olumsuz etkilerinin en aza indirilmesiyle olduğu bilinmektedir (8). Seminal plazma spermatozoon için koruyucu faktörleri içerdiği gibi, duyarlılığını artırıcı faktörleri de bulunduğundan ejaküle edilmiş spermadaki spermatozoa epididimal spermatozoaya göre soğuk şo-kuna daha duyarlıdır ve daha fazla olumsuz etkilenmektedir (22).…”

Section: Introductionunclassified

Köpek spermasının dondurulmasında farklı sulandırıcıların ve bireylerin etkisi*

UYSAL¹

2007

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Bu çalışmada, köpek spermasının dondurulması üzerine farklı sperma sulandırıcıları ve sulandırıcıya katılan bovine serum albuminin (BSA) etkisinin araştırılması amaçlanmıştır. Araştırmada, 2-4 yaşlarında 5 Alman Çoban köpeği kullanılmıştır. Araştırma süresince, haftada iki kez el masajı yöntemiyle her köpekten 10 olmak üzere toplam 50 ejakülat alınmıştır. Nativ spermalarda spermatozoa motilitesi ve spermatozoa yoğunluğu bakımından istatistiksel olarak bireyler arasındaki fark (p<0.01) önemli bulunurken, diğer spermatolojik özellikler yönünden bireyler arasındaki farklılıklar önemsiz (p>0.05) kaydedilmiştir. Dondurulmuş köpek spermalarında mtris-A (gliserol, BSA, yumurta sarısı) ve mtris-B (gliserol, yumurta sarısı) sulandırıcıları için çözdürme sonrası spermatozoa motilitesi (%) 58.0±11.3, 55.8±10.8, anormal spermatozoa oranı (%) 11.1± 5.2, 18.3± 4.5 ve ölü spermatozoa oranı (%) 29.8± 5.5 ve 37.4±6.0 bulunmuştur. Laiciphos-A (gliserol, BSA, yumurta sarısı) ve laiciphos-B (gliserol, yumurta sarısı) için aynı değerler sırasıyla 34.0± 9.8, 36.0 ± 8.7; 11.2± 4.2, 11.0 ± 3.2; ve 27.7± 8.6 ve 28.4±9.4 kaydedilmiştir. Çözdürme sonrası spermatozoa motilitesi, anormal spermatozoa oranı ve ölü spermatozoa oranı yönüyle sulandırıcılar arasındaki farklılıklar istatistiksel olarak önemli (p< 0.001, p<0.01) bulunmuştur.Sonuç olarak spermaların dondurulmasında kullanılan iki değişik sulandırıcıdan mtris çözdürme sonrası spermatolojik özellik-ler üzerine daha olumlu etki yapmıştır. Modifiye trisle dondurulmuş-çözdürülmüş spermalarda bovine serum albuminin spematozoa motilitesini geliştirdiği ve spermatozoa membran bütünlüğünü yumurta sarısından daha iyi koruduğu gözlenmiştir.Anahtar sözcükler: Bovine serum albumin, köpek, kryoprezervasyon, sperma, sulandırıcı. Effect of different extenders and individuals in freezing of dog semenSummary: The aim of this study was to investigate the effect of different semen extender and bovine serum albumine added to extender on freezing of dog semen. In study, 5 German Shepherd dog used for semen collecting were 2-4 years old. During research, a total of 50 ejaculates consist of 10 samples from each dog by massage method twice in a week. Differences among dogs for sperm motility and sperm concentration were significant (p<0.01) and no differences among dogs for other sperm characteristics were found significant (p>0.05) in fresh semen statistically. Post-thawing sperm motility (%) was found 58.0±11.3, 55.8± 10.8, the percentage of abnormal sperm(%) 11.1± 5.2, 18.3± 4.5 and the percentage of dead sperm (%) 29.8±5.5 and 37.4± 6.0 for mtris A (glycerol, BSA, yumurta sarısı) and mtris B (glycerol, egg yolk) in frozen dog semen. respectively. The same spermatological characteristics (%) were recorded as 34. 0± 9.8, 36.0 ± 8.7; 11.2± 4.2, 11.0 ±3 .2 and 27.7± 8.6 and 28.4± 9.4 for laiciphos-A (glycerol, BSA, yumurta sarısı) and for laiciphos-B (glycerol, egg yolk) respectively. Differences among extenders for post-thawing sperm motility, the percentage of abnormal sperm and the percentage of ...

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Effect of freezing–thawing protocols on the performance of semen from three rabbit lines after artificial insemination

Cited by 42 publications

References 26 publications

Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

Comparison of Glycerol, Lactamide, Acetamide and Dimethylsulfoxide as Cryoprotectants of Japanese White Rabbit Spermatozoa

Köpek spermasının dondurulmasında farklı sulandırıcıların ve bireylerin etkisi*

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