Effect of freezing rate on motility, adenosine triphosphate content and fertilizability in beluga sturgeon (Huso huso) spermatozoa

Aramli, Mohammad Sadegh; Golshahi, Karim; Nazari, Rajab Mohammad; Aramli, Salim

doi:10.1016/j.cryobiol.2015.02.001

Cited by 11 publications

(7 citation statements)

References 40 publications

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“…To examine the frozen effects, each cryobiostraw from all the tested groups was immediately thawed for 3-5 sec in a 37°C water bath and the motility parameter was re-evaluated. Tris-HCl buffer (10 mM, PH=8.0) was used as the activating medium (AM) according to the previous method of beluga sturgeon [8]. Sperm motility was recorded by dark-filed microscopy (Olympus, Tokyo, Japan).…”

Section: Evaluation Of Sperm Motilitymentioning

confidence: 99%

“…Vitrification cryopreservation has been recommended to increase the probability of success, and the effective preservation protocols require higher concentrations of cryoprotectants [4,5]. To date, the methods of sperm cryopreservation have been developed in many sturgeon species, including Acipenser baeri [6], A. ruthenus [7], Huso huso [8] and A. persicus [9]. However, these studies also showed that the cryopreservation effects of different frozen extenders on sperm of sturgeon species were varied considerably.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (<i>Acipenser schrenckii</i>)

Zhang¹

2018

AVS

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Development of artificial propagation technology is becoming increasingly important in sturgeon aquaculture whether species recovery efforts or commercial production. The cryopreservation technique of high-quality semen collected during the spawning season could extremely improve reproductive efficiency for year-round availability, especially for off-season use. However, the cryopreservation technique of sperm in Amur sturgeon (Acipenser schrenckii) has not been effectively developed. In the present study, we firstly tested the cryopreservations combination effects of three cryoprotectants, including methanol, dimethyl sulfoxide (DMSO), and propylene glycol (PG) and fresh yolk (Y) addition on sperm motility, fertilization and embryo development in Amur sturgeon. The results indicated that sperm motility was still more than 60% in the MT group but that of the control group was sharply decreased to 4.5% after 72 h of in vitro storage (4°C). The post-thawed sperm motility analysis showed that there was no significant difference between the MT+DMSO group and MT group, but the fertility rate in the MT group was significantly higher with a value of 42.30±2.57(%) than any other experimental groups, including the MT+DMSO group (P<0.05). Meanwhile, we also found that there was no significantly positive effect on post-thawed sperm motility with Y addition. Interestingly, although the results showed that the MT+DMSO group and MT group had similar effects on the post-thawed sperm motility, the MT+DMSO group had higher hatching rate compared to any other tested groups, including the MT group. Meanwhile, we also found that PG as cryoprotectant was unsuitable for sperm cryopreservation of Amur sturgeon. In conclusion, our results provides invaluable basis in further studies for the optimization technology of artificial propagation in Amur sturgeon.

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Section: Evaluation Of Sperm Motilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (<i>Acipenser schrenckii</i>)

Zhang¹

2018

AVS

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“…Currently, cryopreservation has a detrimental effect on the structural and functional parameters of Russian sturgeon spermatozoa [ 5 , 34 , 35 , 51 ]. Many factors influence the success of cryopreservation, including the initial quality of semen, the composition of the cryomedium, the cryoprotectant, the dilution factor, the concentration of spermatozoa used for cryopreservation, speed of freezing and thawing, as well as physiological aspects of sperm, such as species specificity [ 52 , 53 , 54 ]. Protocols cause damage to cell structure and physiology, altering sperm functioning due to cryo-injuries during freezing and thawing [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Russian sturgeon (Acipenser gueldenstaedtii) Semen Quality and Semen Cryopreservation

Igna

Telea²,

Florea³

et al. 2022

Animals

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The alarming decline in sturgeon populations doubled by growing demands for sturgeon products, urge us to prevent the loss of these species. Fish stocking in natural habitats and developing fish farms are viable solutions, which can be successfully implemented with the help of reproductive biotechnologies. Despite the fact that semen cryopreservation is admittedly an important step for saving the Russian sturgeon, a reproducible standard method with good results has yet to be identified. Sperm quality assessment is essential for quantifying the impact of cryopreservation on spermatozoa. The purpose of our study was to provide additional information regarding semen cryopreservation and semen quality evaluation for the Russian sturgeon. Our study method is based on the use of two yolk-free extenders (with different cryoprotectants: DMSO and methanol) for freezing semen, using a simple freezing protocol. Parameters such as volume, concentration, motility, morphology and membrane integrity were evaluated. In conclusion, cryopreservation of Russian sturgeon spermatozoa using an extender containing methanol as cryoprotectant led to high egg fertilization percentages (72.67 ± 5.4%) even if the total motility values recorded for thawed semen were quite low (18–25%). Additionally, we identified two optimal stains for morphological studies and morphometry (Spermac stain kit and Trypan Blue Solution).

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“…Conventional freezing has been applied to the semen from a large range of fish, including sturgeons (e.g. Aramli, Golshahi, Nazari, & Aramli, 2015; Aramli, Golshahi, Nazari, Aramli, & Banan, 2015; Aramli & Nazari, 2014; Rahimi et al., 2015; Shaliutina‐Loginova & Loginov, 2023), salmonids (Ciereszko et al., 2015; Dietrich et al., 2014; Nynca et al., 2021; Sarvi et al., 2006) and carps (Rodina et al., 2007; Ogretmen et al., 2015). This method was also applied to other fish species (De Souza França et al., 2023; Dhanasekar et al., 2022) and mammals (Monteiro et al., 2022; Neuhauser et al., 2019).…”

Section: General Methods For Semen Cryopreservationmentioning

confidence: 99%

A brief review of the methodology and cryoprotectants in selected fish and mammalian species

Aramli,

Sarvi Moghanlou,

Pourahad Anzabi

2024

Reprod Domestic Animals

Self Cite

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Cryopreservation is a valuable technique used to assist in the genetic improvement of cultured stocks and provide a continuous supply of good‐quality semen for artificial insemination. Conserving semen by cryopreservation serves several purposes (e.g. artificial reproductive technologies and species conservation) and is also used in the clinical treatment of human infertility. However, the lifespan of cryopreserved semen is influenced by a range of factors, including storage temperature, cooling rate, chemical composition of the extender, the concentration of cryoprotectant, reactive oxygen species, seminal plasma composition and hygienic control. The choice of cryoprotectant is a vital factor underlying the success of animal semen cryopreservation. In this regard, extensive research has been carried out on various cryoprotectants, such as egg yolk, dimethyl sulfoxide, methanol, ethylene glycol and dimethylacetamide. Recent studies have also described the use of a range of new cryoprotectants for cryopreservation, including compounds of plant origin (soy), amino acids, antifreeze proteins, carbohydrates and cyclodextrins. Moreover, semen cryopreservation and storage require the use of liquid nitrogen or ultralow refrigeration methods for both long‐ and short‐term storage. This review summarizes the general methods used for freezing semen and discusses the use of traditional and newly emerging cryoprotectants (permeable and non‐permeable) for the cryopreservation of semen in selected fish and mammalian species.

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Effect of freezing rate on motility, adenosine triphosphate content and fertilizability in beluga sturgeon (Huso huso) spermatozoa

Cited by 11 publications

References 40 publications

Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (<i>Acipenser schrenckii</i>)

Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (<i>Acipenser schrenckii</i>)

Evaluation of Russian sturgeon (Acipenser gueldenstaedtii) Semen Quality and Semen Cryopreservation

A brief review of the methodology and cryoprotectants in selected fish and mammalian species

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Effect of freezing rate on motility, adenosine triphosphate content and fertilizability in beluga sturgeon (Huso huso) spermatozoa

Cited by 11 publications

References 40 publications

Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (&lt;i&gt;Acipenser schrenckii&lt;/i&gt;)

Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (&lt;i&gt;Acipenser schrenckii&lt;/i&gt;)

Evaluation of Russian sturgeon (Acipenser gueldenstaedtii) Semen Quality and Semen Cryopreservation

A brief review of the methodology and cryoprotectants in selected fish and mammalian species

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Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (<i>Acipenser schrenckii</i>)

Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (<i>Acipenser schrenckii</i>)