Effect of freezing method and storage at −20 °C and −70 °C on prothrombin time, aPTT and plasma fibrinogen levels

Alesci, S.; Borggrefe, Martin; Dempfle, Carl-Erik

doi:10.1016/j.thromres.2008.11.010

Cited by 47 publications

(33 citation statements)

References 17 publications

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“…Even in this case caring physicians unaware of the real patient situation might abstain from appropriate treatments as a consequence of incorrect results due to change in vacuum tubes. The routine coagulation laboratory testing as PT and aPTT are strongly influenced by freezing and storage when compared with fresh samples [18]. Obviously, the variability between brands of coagulation vacuum tubes shown in our study was not influenced by these procedures because all brands were kept frozen at À708C and thawed at the same time [19].…”

Section: Resultsmentioning

confidence: 69%

Sodium citrate vacuum tubes validation

Lima-Oliveira

Lippi

Salvagno

et al. 2013

Blood Coagulation &Amp; Fibrinolysis

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Sometimes in-vitro diagnostic devices (e.g. blood collection tubes) are not validated before use or when the producer's brand is changed. The aim of this study was to validate five brands of sodium citrate vacuum tubes. Blood specimens from 50 volunteers were collected in five different tube brands (I: Venosafe, II: VACUETTE, III: BD Vacutainer, IV: LABOR IMPORT and V: S-Monovette). Routine coagulation tests [activated partial thromboplastin time (aPTT), prothrombin time (PT), and fibrinogen (FIB)] were performed on ACL TOP instrument using HemosIL reagents. The significance of the differences between samples was assessed by paired Student's t-test, set at P < 0.005. Significant differences were observed for: PT when comparing I vs. II, I vs. III, I vs. V, II vs. III, II vs. IV, II vs. V, III vs. IV, III vs. V and IV vs. V; aPTT when comparing I vs. II, I vs. III, I vs. IV, II vs. IV, III vs. IV and IV vs. V. No differences were observed among brands for FIB determination. We suggest that every laboratory management should both standardize the procedures and frequently evaluate the quality of in-vitro diagnostic devices.

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Section: Resultsmentioning

confidence: 69%

Sodium citrate vacuum tubes validation

Lima-Oliveira

Lippi

Salvagno

et al. 2013

Blood Coagulation &Amp; Fibrinolysis

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“…This gap might be because of the differences in samples' storage and assay methods [26,27]. However, sampling procedures and assay methods we used in the present study complied with Schalm's laboratory testing of coagulation disorders [28].…”

Section: Discussionmentioning

confidence: 99%

Age-Related Developmental Clotting Profile and Platelet Aggregation in Foals Over the First Month of Life

Piccione

Arfuso

Quartuccio

et al. 2015

Journal of Equine Veterinary Science

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“…Long-term stability of frozen samples for PT has been demonstrated up to 12 months when stored at À24 C or for 2 years at À74 C, 3 and up to 3 years at À70 C. 11 Activated partial thromboplastin time could similarly be reliably stored for up to 8 months at À24 C or 2 years at À74 C. 3 However, conflicting findings have been reported under similar conditions. The PT levels were found to be prolonged when frozen at À20 C or À70 C with percentage changes exceeding 10% at 1, 2, 3, or 4 months, whereas aPTT was prolonged with less than a 10% change when tested at 1, 2, and 3 but not 4months with storage at À20 C or À70 C. 12 Freezing samples from individuals with elevated PT levels at shorter intervals found samples could be stored at À20 C for up to 24 hours and only for 12 hours in healthy individuals, whereas aPTT showed significant prolongation when stored at À20 C in both healthy and elevated aPTT participants. 4 Zürcher and colleagues demonstrated the significant change in snap-frozen PTs following transportation at ambient temperature at 48 to 52 hours was due to a decrease in factor V:C starting at 24 hours and factor VII:C at 48 hours.…”

mentioning

confidence: 88%

Effect of Freezing Plasma at –20°C for 2 Weeks on Prothrombin Time, Activated Partial Thromboplastin Time, Dilute Russell Viper Venom Time, Activated Protein C Resistance, and d-Dimer Levels

Foshat

Bates

Russo

et al. 2014

Clin Appl Thromb Hemost

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To assess the impact of preanalytical variables of time and temperature on prothrombin time (PT), activated partial thromboplastin time (aPTT), dilute Russell viper venom time (DRVVT), activated protein C resistance (APCR), and d-dimer, samples from 23 healthy individuals and 18 patients having coagulopathy with known abnormal PT and aPTT were collected. Plasma from each individual was separately pooled and aliquoted; the first 2 aliquots were stored at room temperature then analyzed at 2 hours (baseline) and 4 hours postcollection. The remaining aliquots were stored at -20°C and thawed for analysis at 48 hours, 1, and 2 weeks. In both healthy participants and participants with coagulopathy, PT, aPTT, APCR, DRVVT, and D-dimer had no significant changes at 4 and 48 hours, and 1 and 2 weeks postcollection compared to baseline, or the changes were less than 10%. The results indicate PT, aPTT, DRVVT, APCR, and d-dimer can be stored for 2 weeks at -20°C without compromising clinical interpretation in both healthy individuals and individuals with coagulopathy. Increasing storage time will facilitate sample processing from off-site clinics.

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Effect of freezing method and storage at −20 °C and −70 °C on prothrombin time, aPTT and plasma fibrinogen levels

Cited by 47 publications

References 17 publications

Sodium citrate vacuum tubes validation

Sodium citrate vacuum tubes validation

Age-Related Developmental Clotting Profile and Platelet Aggregation in Foals Over the First Month of Life

Effect of Freezing Plasma at –20°C for 2 Weeks on Prothrombin Time, Activated Partial Thromboplastin Time, Dilute Russell Viper Venom Time, Activated Protein C Resistance, and d-Dimer Levels

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