Effect of freezing and storage temperature on stability and antimicrobial activity of an antibiotic mixture used for decontamination of tissue allografts

Abstract

One of the most important risks to be controlled in tissue banking is the infection associated with the clinical use of auto- and allografts. Thus, tissue disinfection protocols are used, in addition to processing in controlled environments. For this purpose, combinations of antibiotics are designed to ensure a broad spectrum of antimicrobial activity. This type of protocol is usually validated by testing its antimicrobial efficacy. In this work, we have studied the effect of several factors on the potential of an antibiotic mixture: container, freezing, storage at 4 °C, storage at - 30 °C and storage at - 80 °C. The molecular stability of the compounds has also been tested, additionally to their efficacy. Our findings show that storage conditions affect the molecular stability of Fungizone and Tobramycin (only in case of frozen storage for the last one). Nevertheless, the solution retains its antimicrobial activity for several weeks. The availability of stored aliquots of disinfectant solution and defining expiry dates for different storage conditions can help to schedule tissue bank tasks.

Machine perfusion combined with antibiotics prevents donor-derived infections caused by multidrug-resistant bacteria

“…The effect of low temperature on the ex vivo bactericidal potency of the antibiotic was negligible. Even though low temperatures are known to affect the pharmacokinetics of various drugs, the stability and bioactivity of antibiotic are nearly unaffected 41–44 . Our ex vivo antibacterial experiment showed that the antibacterial effect of streptomycin and cefoperazone‐sulbactam was nearly identical at 4 and 37°C (Figure S6).…”

“…Even though low temperatures are known to affect the pharmacokinetics of various drugs, the stability and bioactivity of antibiotic are nearly unaffected. [41][42][43][44] Our ex vivo antibacterial experiment showed that the antibacterial effect of streptomycin and cefoperazone-sulbactam was nearly identical at 4 and 37°C (Figure S6). This observation was consistent with the results of HMP treatment combined with high-dose antibiotic for kidney infection.…”

“…In cases of mixed infections or unidentified pathogens, multidrug therapy via an antibiotic cocktail may be helpful. 44 Currently, it is difficult to determine the remnant bacteria in individual organs shortly following MP plus antibiotic treatment; this issue warrants further investigation. Notably, the recipient rats were observed in a short period and the sample sizes of the CRKP combine with antibiotic groups were small.…”

“…Additionally, developing methods to determine the presence or absence of infection accurately within individual organs in donors with bacteremia is necessary. In cases of mixed infections or unidentified pathogens, multidrug therapy via an antibiotic cocktail may be helpful 44 . Currently, it is difficult to determine the remnant bacteria in individual organs shortly following MP plus antibiotic treatment; this issue warrants further investigation.…”

Contamination of tissue allografts from a multiorgan‐multitissue donor colonized by Candida auris

“…17 We have previously observed a relatively rapid degradation process during the refrigerated storage of amphotericin B for several days but, in the present case, it was freshly prepared and used for that donation. 8 Candida species were more frequently grown in cultures from arteries and in samples obtained after disinfection with antibiotics (processing and post-storage, Table 2), and the isolated C. auris strain was sensitive to amphotericin B. This case is unique in our bank in terms of the growth of fungi in pre-and post-disinfection microbiological cultures from the same artery (data not shown).…”