EFFECT OF FOUR BIOACTIVE COMPOUNDS SEPARATELY AND IN COMBINATION WITH Metarhizium anisopliae ON THE ACTIVITY OF SOME HAEMOLYMPH ENZYMES OF Schistocerca gregaria (FORSKAL).

Abstract

The effect of Metarhizium anisopliae var.acridum and four bioactive compounds (Neem,L-Glutamic acid,Schinusmolle and abamectin) in sole treatments and in combination with M. anisopliae var.acridum was studied on activity of Lactate Dehydrogenase Trehalase, Phenol oxidase, and Acid Phosphates in 5 th nymphal instar haemolymph of desert locust. Obtained results showed that the integration of M. anisopliae var. acridumand the Four bioactive compounds caused 100 % mortality after six days post treatments, also caused severe reduction in activity of all studied enzymes. M anisopliae var.acridum treatment caused significant reduction in Lactate Dehydrogenase by the second day after treatment, but all treatments caused significant reduction in Lactate Dehydrogenase by 4 th day post treatment except Neem. In case of trehalase all treatments were significantly lower than control treatment except abamectin two days post treatment, but by day four all treatments were lower than control, while in the 6 th day post treatment S. molle, L-Glutamic acid and Neem treatments caused significant increase in Trehales activity. All treatments caused significant increase in Phenol oxidase activity two days post treatment except L-Glutamic acid and abamectin treatment, while after four days Neem and M.var.acridum treatment were still higher than control, but other treatments were significantly lower than control treatment, by the 6 th day post treatment only L-Glutamic acid caused significant increase in Phenol oxidase activity. In the situation of Acid phosphatase activity two days post treatment abamectin , L-Glutamic acid and M. anisopliaevar.acridum + L-Glutamic acid were significantly lower than control, while after four days all treatments were significantly lower than control, after sex days post treatment only M.anisopliaevar.acridum and L-Glutamic acid were lower than control

Application of Raw Secondary Metabolites From Four Entomopathogenic Fungi Against Chilli Disease Caused by Viruses

“…Nevertheless, the incubation period of the treatment which tended to be slower was the seed soaked in the raw secondary metabolites of M. anisopliae and L. lecanii as 34.22 and 18.71%, respectively, compared to control. This is accordance to the result of Farrag et al (2015), that M. anisopliae alone or in combination with neem or L-Glutamic acid were no significant differences to control treatment, even the control was significantly higher than M. anisopliae treatment. The raw secondary metabolites produced in M. anipsoliae differ from other entomopathogenic fungi (Isaka et al, 2005).…”