Effect of Forskolin onBradykinin-Induced Calcium Mobilizationin Cultured Canine Tracheal Smooth Muscle Cells

Luo, Shue Fen; Chiu, Chi Tso; Tsao, Hui Liang; Fan, Lir Wan; Tsai, Chuen Tao; Pan, Shiow Lin; Yang, Che‐Hua

doi:10.1016/s0898-6568(96)00136-2

Cited by 8 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding may also be supported by some previous reports that the activation of PKA has an inhibitory effect on the BK‐dependent production of inositol triphosphate and Ca 2+ mobilization (Campbell et al. 1990; Luo et al. 1997).…”

Section: Discussionsupporting

confidence: 85%

Molecular cloning of prostaglandin EP3 receptors from canine sensory ganglia and their facilitatory action on bradykinin‐induced mobilization of intracellular calcium

Kozaki

Kambe

Hayashi

et al. 2006

Journal of Neurochemistry

View full text Add to dashboard Cite

We previously demonstrated that the activation of prostaglandin E-prostanoid-3 (EP3) receptor sensitized the canine nociceptor response to bradykinin (BK). To elucidate the molecular mechanism for this sensitization, we cloned two cDNAs encoding EP3s with different C-terminals, from canine dorsal root ganglia, and established the transformed cell lines stably expressing them. In both transformants, EP3 agonist did not increase intracellular cAMP levels, but it attenuated forskolin-dependent cAMP accumulation in a pertussis toxin (PTX)-sensitive manner and increased intracellular calcium levels in a PTX-resistant manner, indicating that both EP3s can couple with Gi and Gq, but not with Gs proteins. As the nociceptor response to BK is mediated by BK B2 receptor, it was transfected into the transformants and the effects of EP3 agonist on BK-dependent calcium mobilization were investigated. When BK was applied twice with a 6-min interval, the second response was markedly attenuated. Pre-treatment with EP3 agonist had no effect on the initial response, but restored the second response in a PTX-sensitive manner. A protein kinase A inhibitor mimicked the effect of EP3 agonist. These results demonstrate that the activation of EP3 restores the response to BK by attenuating the desensitization of BK B2 receptor activity via Gi protein.

show abstract

Section: Discussionsupporting

confidence: 85%

Molecular cloning of prostaglandin EP3 receptors from canine sensory ganglia and their facilitatory action on bradykinin‐induced mobilization of intracellular calcium

Kozaki

Kambe

Hayashi

et al. 2006

Journal of Neurochemistry

View full text Add to dashboard Cite

show abstract

“…This lack of an effect might either be the result of the absence of a cAMP increase in caOECs or be attributable to the distinct roles that the cyclic nucleotides might play inside the canine cell. Since forskolin has been shown to increase the intracellular cAMP level in a variety of canine cell types in vitro, e.g., thyrocytes, myocytes, and epithelial cells (Chu et al 2004;Van Keymeulen et al 2000;Luo et al 1997;Zhang et al 2000), the latter possibility seems more likely. This indicates that rOECs and caOECs differ with respect to the intracellular signalling cascades regulating cellular proliferation.…”

Section: Purification and In Vitro Characterization Of Adult Caoecsmentioning

confidence: 99%

Purification and in vitro characterization of adult canine olfactory ensheathing cells

Krudewig¹,

Deschl

Wewetzer

2006

Cell Tissue Res

View full text Add to dashboard Cite

Olfactory ensheathing cells (OECs) are known to promote neural repair under experimental conditions. The experimental focus has so far been almost entirely on rodent OECs (rOECs), and hence whether human OECs (humOECs) display similar properties is unclear. Studies on larger mammals as an "intermediate" model may be helpful for translating the experimental evidence gathered so far into novel therapeutic strategies. In the present study, we purified adult canine OECs (caOECs) from the olfactory bulb and analyzed their in vitro properties with respect to antigen expression, proliferation, and differentiation. Secondary caOECs shared the expression of marker molecules and the reactivity toward growth factors, with rOECs and humOECs. CaOECs were positively immunostained for the low affinity neurotrophin receptor p75, GFAP, and O4 and proliferated in response to fibroblast growth factor-2 and heregulin-1β. No decline in proliferation was noted at higher passages (>8). The effects of forskolin, which neither increased proliferation nor stimulated the expression of O4, were clearly different from those on rOECs. Moreover, caOECs displayed their typical spindle-shaped morphology only upon growth factor/forskolin addition, whereas mitotically quiescent caOECs had a flattened morphology. Thus, caOECs can readily be purified from adult canine olfactory bulb and expanded by using established OEC mitogens. The behavior of caOECs toward forskolin suggests that caOECs and humOECs share a number of properties amd implies the presence of common intracellular signalling pathways. CaOECs therefore represent a suitable model system relevant for humOECs in neural repair studies.

show abstract

“…A previous study reported that B2R activates the PLC/PKC and cAMP/PKA pathways, and cAMP is known to inhibit cell growth [ 66 ]. Another study reported that cAMP/PKA pathway activation suppresses B2R and leads to the reduction of bradykinin-dependent inositol triphosphate and Ca 2+ mobilization [ 67 , 68 , 69 ]. In the present study, the expression of B2R and CaMKIIδ in cumulus cells from the 20 nM GT1b treatment group was significantly decreased compared with the control.…”

Section: Discussionmentioning

confidence: 99%

Effect of ganglioside GT1b on the <i>in vitro</i> maturation of porcine oocytes and embryonic development

Hwang

Jeon

Yoon

et al. 2015

Journal of Reproduction and Development

View full text Add to dashboard Cite

Ganglioside is an acidic glycosphingolipid with sialic acids residues. This study was performed to investigate the effect and mechanism of ganglioside GT1b in porcine oocytes in the process of in vitro maturation (IVM) and preimplantation development. Metaphase II (MII) rates were significantly (P < 0.05) different between the control group and the 5 nM GT1b treatment group. Intracellular glutathione (GSH) levels in oocytes matured with 5 nM and 20 nM and GT1b decreased significantly (P < 0.05). The 10 nM group showed a significant (P < 0.05) decrease in intracellular reactive oxygen species (ROS) levels compared with the control group. Subsequently, the level of intracellular Ca2+ in oocytes treated with different concentrations of GT1b was measured. Intracellular Ca2+ was significantly (P < 0.05) increased with a higher concentration of GT1b in a dose-dependent manner. Real-time PCR was performed and showed that the expression of bradykinin 2 receptor (B2R) and calcium/calmodulin-dependent protein kinase II delta (CaMKIIδ) in cumulus cells was significantly (P < 0.05) decreased in the 20 nM GT1b treatment group. Treatment with 5 nM GT1b significantly (P < 0.05) decreased the expression of CaMKIIδ. In oocytes, treatment with 5 nM GT1b significantly (P < 0.05) decreased CaMKIIγ and POU5F1 (POU domain, class 5, transcription factor 1). However, treatment with 20 nM GT1b significantly (P < 0.05) increased the expression of POU5F1. Finally, embryonic developmental data showed no significant differences in the two experiments (parthenogenesis and in vitro fertilization). In conclusion, the results of the present study indicated that GT1b plays an important role in increasing the nuclear maturation rate and decreasing the intracellular ROS levels during IVM. However, GT1b inhibited maturation of the cytoplasm by maintaining intracellular Ca2+ in the process of oocyte maturation regardless of the cell cycle stage. Therefore, GT1b is thought to act on another mechanism that controls intracellular Ca2+.

show abstract

Effect of Forskolin onBradykinin-Induced Calcium Mobilizationin Cultured Canine Tracheal Smooth Muscle Cells

Cited by 8 publications

References 27 publications

Molecular cloning of prostaglandin EP3 receptors from canine sensory ganglia and their facilitatory action on bradykinin‐induced mobilization of intracellular calcium

Molecular cloning of prostaglandin EP3 receptors from canine sensory ganglia and their facilitatory action on bradykinin‐induced mobilization of intracellular calcium

Purification and in vitro characterization of adult canine olfactory ensheathing cells

Effect of ganglioside GT1b on the <i>in vitro</i> maturation of porcine oocytes and embryonic development

Contact Info

Product

Resources

About