Effect of formulation on the rhizosphere competence and biocontrol ability of Trichoderma atroviride C52

Abstract

The rhizosphere competence of the biological control agent Trichoderma atroviride isolate C52 was studied on onion roots both in the glasshouse and in the field when introduced into soil in a range of formulations. Proliferation of T. atroviride in the rhizosphere was formulation-dependent. A pellet formulation maintained the fungal concentration at 10 5 cfu per g soil, whereas solid-substrate and seed-coating formulations gave concentrations of 10 4 and 10 1 cfu per g soil, respectively.To facilitate rhizosphere-competence studies, a UP-PCR band profile generated with primer L45 for isolate C52 was used to enable conclusive identification of T. atroviride C52 when recovered from soil. When isolate C52 was introduced into Sclerotium cepivorum -infested soil as both pellet and solid-substrate formulations, there was no statistically significant difference in the disease control between these treatments, but the pellet treatment doubled the percentage of healthy plants compared with the control treatment.

Biocontrol Potential of Local Trichoderma atroviride Isolated from Bihar, India against Fungal Plant Pathogens Aspergillus flavus and Fusarium solani

“…This result matches with the research finding by McLean et al, (2005). The isolates of Trichoderma atroviride showed excellent antagonistic activity against the fungal plant pathogens Aspergillus flavus and Fusarium solani causing rot diseases in various crops.…”

Field Demonstration of Trichoderma harzianum as a Plant Growth Promoter in Wheat (Triticum aestivum L)

“…This study can be supported by findings of other workers reporting the role of Trichoderma as growth promoter. Several studies have documented that Trichoderma harzianum sensu lato, T. asperellum and T. asperelloides are highly rhizosphere competent and are able to stimulate growth and immune defense of plants (Harman et al, 2004). Mc Lean et al (2005 reported colonization and proliferation of Trichoderma atroviride (C52) when applied as bioformulation in onions against Sclerotium cepivorum providing nutrient to the biocontrol agent as compared to a very low colony count when applied only on seed.…”

Testing of Eight Medicinal Plant Extracts in Combination with Kresoxim-Methyl for Integrated Control of Botrytis cinerea in Apples

“…Interestingly, even though the E. rhinocerotis extract showed synergistic effects in the in vivo study, it did not produce a similar effect in the in vitro study. This could be a result of the type of biological formulation used, which could have affected the efficacy of the extract in the in vitro study [39,40].…”