Effect of formulated root endophytic fungus Piriformospora indica and plant growth promoting rhizobacteria fluorescent pseudomonads R62 and R81 on Vigna mungo

Kumar, Vinod; Sarma, M.V.R.K.; Saharan, Krishna; Srivastava, Rashmi; Kumar, Lalit; Sahai, Vaibhav; Bisaria, Virendra S.; Sharma, Anita

doi:10.1007/s11274-011-0852-x

Cited by 54 publications

(33 citation statements)

References 30 publications

(39 reference statements)

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“…Results of the current investigation suggest that mixed inoculation and inoculation with the co-culture of P. indica and B. pumilus are more efficient than single inoculation of the biological agents for improving seedling growth in tomato. Previous reports involving other crops also supported the idea of co-inoculation of P. indica with beneficial bacteria to achieve greater plant growth (Meena et al 2010;Sarma et al 2011;Kumar et al 2012). …”

Section: Discussionmentioning

confidence: 70%

“…P. indica has been applied with beneficial bacterial strains such as PGPR as a mixed inoculum for mung bean and tomato. This resulted in improved growth response and disease suppression ability (Sarma et al 2011;Kumar et al 2012). Improved plant growth in chick pea has also been reported for the combined inoculation involving P. indica and the phosphate solubilizing bacterium Pseudomonas striata (Meena et al 2010).…”

Section: Introductionmentioning

confidence: 73%

“…If seedlings are already treated with the biological agents while in plug trays, the further multiplication of the agents may be expected during crop growth without additional application (Yan et al 2003;Russo 2006a, b;Russo and Perkins-Veazie 2010). The endophytic fungus P. indica is usually mass multiplied in potato dextrose broth for plant growth promotion experiments and inoculum preparations (Fakhro et al 2010;Anith et al 2011;Sarma et al 2011;Kumar et al 2012). In the present study, the fungus grew well on solid CWA, and autoclaved coconut water (ACW) was found to be suitable for mass multiplication of P. indica.…”

Section: Discussionmentioning

confidence: 99%

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The growth of tomato seedlings inoculated with co-cultivated Piriformospora indica and Bacillus pumilus

Anith

Sreekumar

2015

Symbiosis

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A novel co-culture method using the beneficial root endophytic fungus Piriformospora indica and a plant-growth promoting rhizobacterial strain of Bacillus pumilus to promote the growth of tomato seedlings in plug trays, is described. Coconut water, a waste product from the coconut industry, was used as medium for co-cultivation of the two biological agents. During the co-culture with the fungus, the bacterial strain showed a similar growth rate to that in monoculture in fresh autoclaved coconut water. In contrast, co-culture in potato dextrose broth (PDB), a medium routinely used for cultivation of the endophytic fungus, did not support bacterial growth. Inoculation with the co-culture or a mixture of P. indica and B. pumilus promoted tomato seedling growth significantly when compared with individual application of the two biological agents. No difference was observed with respect to the degree of root colonization in tomato seedlings whether it was done with a monoculture or a co-culture with rhizobacterium. The co-culture system developed reduces the cost of inoculum production as it uses coconut water, a cheap and locally available waste product and a single fermentation vessel. Further research is required to know the endophytic nature of the rhizobacterial strain and its possible role in helping the fungus in root colonization.

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Section: Discussionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

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The growth of tomato seedlings inoculated with co-cultivated Piriformospora indica and Bacillus pumilus

Anith

Sreekumar

2015

Symbiosis

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“…The number of studies investigating the efficacy of such combined inoculations is growing, with variations in the number of microorganisms and the nature of the combinations (PGPR strains only, PGPR and fungi, etc. ; Cassán et al, 2009; Couillerot et al, 2012; Kumar et al, 2012; Walker et al, 2012). Field inoculation of sorghum with fluorescent Pseudomonas strains alone or in combination with arbuscular mycorrhizal fungi showed a better effect when in presence of the latter (Kumar et al, 2012).…”

Section: Expression Of Plant-beneficial Functions Of Pgpr In the Rhizmentioning

confidence: 99%

Plant growth-promoting rhizobacteria and root system functioning

Vacheron¹,

Desbrosses²,

Bouffaud³

et al. 2013

Front. Plant Sci.

1,125

647

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The rhizosphere supports the development and activity of a huge and diversified microbial community, including microorganisms capable to promote plant growth. Among the latter, plant growth-promoting rhizobacteria (PGPR) colonize roots of monocots and dicots, and enhance plant growth by direct and indirect mechanisms. Modification of root system architecture by PGPR implicates the production of phytohormones and other signals that lead, mostly, to enhanced lateral root branching and development of root hairs. PGPR also modify root functioning, improve plant nutrition and influence the physiology of the whole plant. Recent results provided first clues as to how PGPR signals could trigger these plant responses. Whether local and/or systemic, the plant molecular pathways involved remain often unknown. From an ecological point of view, it emerged that PGPR form coherent functional groups, whose rhizosphere ecology is influenced by a myriad of abiotic and biotic factors in natural and agricultural soils, and these factors can in turn modulate PGPR effects on roots. In this paper, we address novel knowledge and gaps on PGPR modes of action and signals, and highlight recent progress on the links between plant morphological and physiological effects induced by PGPR. We also show the importance of taking into account the size, diversity, and gene expression patterns of PGPR assemblages in the rhizosphere to better understand their impact on plant growth and functioning. Integrating mechanistic and ecological knowledge on PGPR populations in soil will be a prerequisite to develop novel management strategies for sustainable agriculture.

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“…Despite this strong indication of a direct contribution of P. indica to plant P nutrition, experimental results on agricultural crops have been inconsistent. While in tobacco, barley and green gram, colonization by P. indica did not increase P content of plants despite plant growth promotion (Barazani et al 2007;Achatz et al 2010;Ray and Valsalakumar 2010), chickpea and black lentil plants showed higher P content (Nautiyal et al 2010;Kumar et al 2012). This inconsistency may be partly explained by differences in experimental conditions (Fakhro et al 2010) and highlights the need for further research.…”

Section: Introductionmentioning

confidence: 99%

Phosphate utilization by the fungal root endophyte Piriformospora indica

et al. 2015

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Background and aim The root endophytic fungus Piriformospora indica increases plant resistance and tolerance to stress and promotes plant growth, but its ability to support plant nutrition is still controversially discussed. Irrespective of a potential nutrient transport towards the plant, the fungus might release P from sources unavailable for plant usage by transformation to available forms. Methods To test this hypothesis, sterile solid and liquid in vitro cultures of P. indica supplied with different organic and inorganic P sources were established. Cultures were investigated for growth, solubilised P, enzyme activities, RNA accumulation of the four genes encoding phosphate transporters and the two genes for acid phosphatases and phytases respectively found in P. indica genome, and for pH values in the media. Results P. indica growth was higher in the presence of inorganic P than in organic P sources. Significant amounts of P were solubilised by P. indica from Ca 3 (PO 4 ) 2 and rock phosphate. However, no relevant intra-or extracellular enzymatic activity was detected despite RNA accumulation of related genes. In general, the genes were all repressed by higher amounts of inorganic P and were expressed the most when the fungus received phytate. We observed a decrease in medium pH in the presence of P. indica irrespective of the P source. Conclusions P. indica is able to solubilise phosphate from inorganic, but not from organic P sources. This P solubilisation is not due to enzymatic activities but rather to the lowering of the medium pH.

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Effect of formulated root endophytic fungus Piriformospora indica and plant growth promoting rhizobacteria fluorescent pseudomonads R62 and R81 on Vigna mungo

Cited by 54 publications

References 30 publications

The growth of tomato seedlings inoculated with co-cultivated Piriformospora indica and Bacillus pumilus

The growth of tomato seedlings inoculated with co-cultivated Piriformospora indica and Bacillus pumilus

Plant growth-promoting rhizobacteria and root system functioning

Phosphate utilization by the fungal root endophyte Piriformospora indica

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