Effect of Food on Porcine Gut Regulatory Peptides in Arterial and Portal Venous Blood

Manolas, Konstantinos J; Adrian, Thomas E.; Dunlop, H.M.; Kamilaris, Th.C.; Bacarese-Hamilton, A.J.; Bloom, S.R.; Welbourn, Richard

doi:10.1159/000180107

Cited by 4 publications

(2 citation statements)

References 14 publications

(17 reference statements)

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“…After boiling for 10 min the extracts were stored at -20°C and centrifuged after thawing before radioimmunoassay and chromatography. Circulating PYY concentrations Six pigs (Danish Landrace) were studied after cannulating an iliac artery and vein and the portal vein under general anaesthesia as previously described in detail (Manolas, Adrian, Dunlop et al 1985). Post¬ prandial PYY secretion studies were carried out 10 days after cannulation, the pigs having been fasted for 20 h overnight.…”

Section: Tissue Distributionmentioning

confidence: 99%

Distribution and postprandial release of porcine peptide YY

Adrian¹,

Bacarese-Hamilton²,

Smith³

et al. 1987

Journal of Endocrinology

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Peptide YY (PYY), a thirty-six amino acid intestinal hormonal peptide with a tyrosine residue at each end (hence YY as Y represents tyrosine in the new peptide nomenclature), was found throughout the gastrointestinal tract of the pig. Concentrations were very low in the foregut (antrum, 3.4 +/- 0.3 pmol/g; duodenum, 1.1 +/- 1.5 pmol/g), higher in the distal small intestine (ileum, 100 +/- 13 pmol/g) and very high in the large bowel (descending colon, 270 +/- 45 pmol/g). Peptide YY was found to circulate in plasma and concentrations rose substantially in response to eating (fasting, 138 +/- 15 pmol/l; postprandial, 263 +/- 21 pmol/l; P less than 0.001). There was a small but significant portal/arterial gradient in postprandial PYY levels. More than 90% of the immunoreactive PYY in gut extracts eluted, on gel permeation chromatography, in an identical position to pure PYY standard, but small amounts of higher molecular weight material, possibly precursors, were detected. In contrast, plasma from fasting pigs contained a large proportion (60-70%) of these large molecular forms. These findings suggest that the putative pro-PYY may be cleared more slowly from the circulation than the 36 amino acid hormonal peptide. The high concentrations of immunoreactive PYY in the circulation of the young pig may reflect a species difference between pig and man or may indicate an important role for PYY in the developing animal.

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Section: Tissue Distributionmentioning

confidence: 99%

Distribution and postprandial release of porcine peptide YY

Adrian¹,

Bacarese-Hamilton²,

Smith³

et al. 1987

Journal of Endocrinology

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“…Τα κυριότερα ερεθίσματα για τη σύνθεση και απελευθέρωση των ορμονών από τα κύτταρα αυτά είναι η θέα της^τροφής, η μάσηση -κατάποση της (εντο λές που μεταβιβάζονται με το κεντρικό και περιφερικό νευρικό σύστημα) και η διάταση του στομάχου -εντέρου από την παρουσία της τροφής 20 • 21 .…”

Section: φυσιολογια του παγκρεατος ενδοκρινη! λειτουργιαunclassified

Τα Επιπεδα Της Αμμωνιας Στο Φλεβικο Αιμα Του Παγκρεατος Και Του Δωδεκαδακτυλου Μετα Απο Χορηγηση Γλυκοζης Και Εκκριματινης. (Πειραματικη Εργασια Επι Κυνων)

Φαρδελλασ¹

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Effects of jejunoileal autotransplantation on gastrointestinal regulatory peptides

et al. 1994

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Plasma gastrointestinal hormones were measured before and during feeding in eight dogs, more than one year after total autotransplant of the entire jejunoileum, and in controls. At sacrifice, tissues were taken from the transplanted segment and intact bowel for measurement of hormone and enteric neuropeptide content. Gastrin levels were reduced in autotransplanted dogs (fasting 63% of control, incremental response 67% of control, both P < 0.05), reflecting the loss of acid inhibitory reflexes. Secretin and cholecystokinin responses were identical between the two groups. Postprandial levels of gastric inhibitory peptide (incremental response 175% of control, P < 0.005), insulin, and peptide YY (158% of control, P < 0.05) were elevated following denervation, the former suggesting more rapid gastric emptying while the latter may reflect malabsorption. The neurotensin meal response was obtunded by denervation (incremental response 43% of control, P < 0.05), providing evidence for a neural pathway for its release. Pancreatic polypeptide responses were identical between the groups, suggesting intact pancreatic innervation. Abnormal hormone secretion may contribute to the impaired fed motor responses seen following extrinsic denervation of the small bowel. In contrast, the neuropeptide content of the autotransplanted small intestine is normal, suggesting that extrinsic denervation has no long-term effects on peptide content of the enteric nervous system.

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Effect of Food on Porcine Gut Regulatory Peptides in Arterial and Portal Venous Blood

Cited by 4 publications

References 14 publications

Distribution and postprandial release of porcine peptide YY

Distribution and postprandial release of porcine peptide YY

Τα Επιπεδα Της Αμμωνιας Στο Φλεβικο Αιμα Του Παγκρεατος Και Του Δωδεκαδακτυλου Μετα Απο Χορηγηση Γλυκοζης Και Εκκριματινης. (Πειραματικη Εργασια Επι Κυνων)

Effects of jejunoileal autotransplantation on gastrointestinal regulatory peptides

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