This study was conducted inside the plastic house of the Agricultural Research and Experiment Station of the Agriculture College - University of Kirkuk/Iraq, during the growing season 2020/2021. To study the effect of spraying the growth regulator of benzyl adenine in three concentrations (0, 30, 60) mg.L−1 and licorice root extract in three concentrations (0, 3000, 6000) mg.L−1 on some growth characteristics and chemical content of strawberry plants of Rubygem type. The obtained results were summarized that spraying with a concentration of 60 mg.L−1 of the growth regulator benzyl adenine BA led to a significant superiority in most growth characteristics and chemical content of strawberry plants (leaf area, leaves number, leaves area, dry matter in plant, leaf content of chlorophyll and nitrogen total) where reached (95.81 cm2, 15.78 leaf. plant−1, 1513.93 cm2, 38.68 %, 28.66 %, 3.24 %) respectively. While spraying with concentrations (30 and 60 mg.L−1)of benzyl adenine was significantly superior to the control treatment in the phosphorous and carbohydrates content of leaves. Spraying with a concentration of 6000 mg.L−1 of licorice extract significantly increased in most of the study traits (dry matter in plant, leaf content of chlorophyll, nitrogen, phosphorous and carbohydrates total) where reached (39.06 %, 25.69 mg.g−1, 3.09 %, 0.334 %, 3.38 %) respectively. Spraying with a concentration of 3000 mg.L−1 of licorice was significantly superior in the characteristics of leaf area and Leaves area, which reached (85.08, 1303.18) cm2 respectively.