Effect of Fluoride on Hypervitaminosis D in Rats

Abstract

SYNOPSIS IN INTERLINGUA LE EFFECTO DE FLUORURO SUPER HYPERVITAMINOSIS D IN RATTOS.-Esseva usate rattos recipients 0,2 mg de fluoruro per die (i.e., approximativemente le ingestion physiologic pro le ratto), rattos recipients doses toxic de vitamina D, rattos recipients vitamina D e fluoruro simultaneemente, e rattos de control. Esseva effectuate determinationes de calcium in le sero e le urina e del gravitate specific e del contento de cinere in le femure si hen como su contento de calcium. Le resultatos revelava que fluoruro age contra le syndrome de hypervitaminosis D per reducer usque ad valores quasi normal le concentrationes de calcium in le sero e le urina e etiam que illo preveni le resorption de osso. Es suggestionate que in hypervitaminosis D, le presentia de un augmentate concentration de iones de fluoruro in le liquidos del tissus induce un precipitation de fluoro-apatite.The toxic effects of excessive amounts of vitamin D on rats have been studied by many investigators. The main effects found were an increased rate of bone resorption, accompanied by hypercalcemia and histologic changes in bones and teeth.'-3It has often been stated that increased fluoride (F) intake hypermineralizes preformed bone.4 Some investigators were able to reduce the toxic effects produced by high doses of fluoride in rats by administering vitamin D in amounts greater than the normal physiologic requirement.5The purpose of this study was to investigate the effect of fluoride administration in hypervitaminosis D. Materials and MethodsForty-two male and female rats of the Sabra strain (Hebrew University), weighing 170 + 20 Gm., were used. They were maintained on a standard mixed diet containing 3.2 ppm F and were provided with drinking water containing 0.5 to 0.6 ppm of F. This diet is considered to be low in fluoride for rats.7 The rats were allotted to 6 groups ( Table 1): Group 1-4 rats were used as controls. Group 2-10 rats each received 500,000 USP units of vitamin D2 (12.5 mg.) daily, in a single subcutaneous dose for 4 consecutive days. Group 3-6 rats each received 1 ml. of 0.04 per cent NaF solution (0.2 mg. of F) intraperitoneally, daily, for 4 consecutive

Effects of Fluoride Intake on Disuse Atrophy of Bone in Rats

“…After defatting and drying both femora, their specific gravity, ash content and the calcium and fluoride contents of the ash were determined as previously described ( 5). Using aseptic technique, the femoral nerve was severed in the area of the femoral triangle.…”

Effect of Calcium and Vitamin D on Fluoride Metabolism in the Rat

“…Dietary fluoride has been reported to stimulate new bone formation [1,2] and prevent bone absorption [3], The effect of fluoride on long-term retention has not been investigated.…”