Effect of fluid shear stress on the internalization of kidney-targeted delivery systems in renal tubular epithelial cells

Xu, Yingying; Qin, Shuo; Niu, Yining; Gong, Tao; Zhang, Zhirong; Fu, Yao

doi:10.1016/j.apsb.2019.11.012

Cited by 33 publications

(30 citation statements)

References 30 publications

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“…We detected nan in one TOG neuron and one DOG neuron, whereas nompC and pain label a larger population. We recorded responses of pain-expressing GSNs exposed to water flow turned on and off, as we intended to create fluid shear stress on the cell membrane, similarly to observations on cultured cells (84) and studies on vascular tissue (85). It remains to be elucidated if shear stress responses detected in pain neurons are mediated by Painless channel itself.…”

Section: Discussionmentioning

confidence: 99%

Multimodal and multisensory coding in theDrosophilalarval peripheral gustatory center

Maier

Biočanin

Bues

et al. 2020

Preprint

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The ability to evaluate food palatability is innate in all animals, ensuring their survival. The external taste organ in Drosophila larvae is composed of only few sensory neurons but enables discrimination between a wide range of chemicals and displays high complexity in receptor gene expression and physiological response profile. It remains largely unknown how the discrepancy between a small neuronal number and the perception of a large sensory space is genetically and physiologically resolved. We tackled dissection of taste sensory coding at organ level with cellular resolution in the fruit fly larva by combining whole-organ calcium imaging and single-cell transcriptomics to map physiological properties and molecular features of individual neurons. About one third of gustatory sense neurons responded to multiple tastants, showing a rather large degree of multimodality within the taste organ. Further supporting the notion of signal integration at the periphery, we observed neuronal deactivation events within simultaneous neighboring responses, suggesting inter-cellular communication through electrical coupling and thus providing an additional level in how neurons may encode taste sensing. Interestingly, we identified neurons responding to both mechanical and taste stimulation, indicating potential multisensory integration. On a molecular level, chemosensory cells show heterogeneity in neuromodulator expression. In addition to a broad cholinergic profile, markers on dopaminergic, glutamatergic or neuropeptidergic pathways are present either in distinct cell populations or are seemingly co-expressed. Our data further extend the sensory capacity of the larval taste system pointing towards an unanticipated degree of multimodal and multisensory coding principles.

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Section: Discussionmentioning

confidence: 99%

Multimodal and multisensory coding in theDrosophilalarval peripheral gustatory center

Maier

Biočanin

Bues

et al. 2020

Preprint

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“…Fluorescence images were taken on a confocal microscope (LSM 800, Carl Zeiss, Jena, Germany) obtaining 18 images per section. As previously mentioned 33 , the average fluorescence intensity was quantitated by ImageJ (NIH, Bethesda, USA). The mean gray value (MOD) of positive staining in the visual field of each slide was calculated respectively.…”

Section: Methodsmentioning

confidence: 99%

An injectable micelle-hydrogel hybrid for localized and prolonged drug delivery in the management of renal fibrosis

Qin

et al. 2021

Acta Pharmaceutica Sinica B

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Localized delivery, comparing to systemic drug administration, offers a unique alternative to enhance efficacy, lower dosage, and minimize systemic tissue toxicity by releasing therapeutics locally and specifically to the site of interests. Herein, a localized drug delivery platform (“plum‒pudding” structure) with controlled release and long-acting features is developed through an injectable hydrogel (“pudding”) crosslinked via self-assembled triblock polymeric micelles (“plum”) to help reduce renal interstitial fibrosis. This strategy achieves controlled and prolonged release of model therapeutics in the kidney for up to three weeks in mice. Following a single injection, local treatments containing either anti-inflammatory small molecule celastrol or anti-TGF β antibody effectively minimize inflammation while alleviating fibrosis via inhibiting NF- κ B signaling pathway or neutralizing TGF- β 1 locally. Importantly, the micelle-hydrogel hybrid based localized therapy shows enhanced efficacy without local or systemic toxicity, which may represent a clinically relevant delivery platform in the management of renal interstitial fibrosis.

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“…Megalin is a low-density lipoprotein (LDL) receptor that is expressed in epithelial cells of the intestine, kidney, and other tissues (Christensen and Birn, 2002;Nielsen et al, 2016). On the apical plasma membrane of proximal tubule cells, it binds with cubilin (intestinal intrinsic factor-cobalamin receptor) and can mediate the endocytosis of many ligands including lipoproteins, hormones, enzymes, lysozyme, carrier proteins, drugs (such as aminoglycoside and polymyxin B and chitosan) (Christensen and Birn, 2002;Lin et al, 2013;Oroojalian et al, 2017;Xu et al, 2020).…”

Section: Molecular Recognition and Endocytosismentioning

confidence: 99%

Targeted Drug Delivery Systems for Kidney Diseases

Huang

et al. 2021

Front. Bioeng. Biotechnol.

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Kidney diseases have gradually become a global health burden. Along with the development of nanotechnology, many hybrids or nanomaterials have been utilized to promote treatment efficiency with negligible side effects. These therapeutic agents have been successfully applied in many fields. In particular, some efforts have also been made to ameliorate the treatment of kidney diseases through targeted delivery nanomaterials. Though most of the delivery systems have not yet been transmitted into clinical use or even still at an early stage, they have shown great potential in carrying immunosuppressants like tacrolimus and triptolide, antioxidants, or siRNAs. Excitingly, some of them have achieved significant treatment effectiveness and reduced systemic side effect in kidney disease animal models. Here, we have reviewed the recent advances and presented nanotherapeutic devices designed for kidney targeted delivery.

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Effect of fluid shear stress on the internalization of kidney-targeted delivery systems in renal tubular epithelial cells

Cited by 33 publications

References 30 publications

Multimodal and multisensory coding in theDrosophilalarval peripheral gustatory center

Multimodal and multisensory coding in theDrosophilalarval peripheral gustatory center

An injectable micelle-hydrogel hybrid for localized and prolonged drug delivery in the management of renal fibrosis

Targeted Drug Delivery Systems for Kidney Diseases

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