Effect of five flavonoid compounds isolated from leaves of Diospyros kaki on stimulus-induced superoxide generation and tyrosyl phosphorylation of proteins in human neutrophils

Chen, Guang; Lu, Huangwei; Wang, Chunlei; Yamashita, Koichi; Manabe, Masanobu; Zhang, Meng; Xu, Sui-Xu; Kodama, Hiroyuki

doi:10.1016/s0009-8981(02)00298-x

Cited by 29 publications

(20 citation statements)

References 20 publications

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“…2b). These results are in agreement with the observation of Chen et al (2002), who showed that compound 3 at a high concentration can suppress superoxide generation, induced by phorbol 12-myristate 13-acetate (PMA) and formyl-methionyl-leucyl-phenylalanine (fMLP), in a dose dependant manner. However, the system we employed (serum opsonized luminol dependant-chemiluminescence) can detect varieties of free radicals, such as superoxide, H 2 O 2 , OH, and HOCl (McNally and Bell, 1996).…”

Section: Resultssupporting

confidence: 92%

Bioactive flavonoids and saponins from Climacoptera obtusifolia

et al. 2006

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Section: Resultssupporting

confidence: 92%

Bioactive flavonoids and saponins from Climacoptera obtusifolia

et al. 2006

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“…Major constituents of D. kaki leaves responsible for pharmacological effect are flavonoids and terpenoids (1,(4)(5)(6)(7). Therefore, although we could not pinpoint the exact active compound present in acetone extract of KV-1, flavonoid and/or terpenoid compound might be responsible for ATRA and 1,25-(OH) 2 D 3 -induced HL-60 differentiation.…”

Section: Discussionmentioning

confidence: 84%

Effects and action mechanism of Diospyros kaki on the differentiation of human leukemia HL-60 cells

et al. 2009

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Abstract. Diospyros kaki] or all-trans retinoic acid (ATRA). Combination of low doses of ATRA or 1,25-dihydroxyvitamin D 3 that do not induce toxicity with another drug is a useful strategy for acute promyelocytic leukemia therapy. Our main aim was to investigate the effect of an acetone extract of D. kaki leaves (KV-1) on HL-60 cell differentiation in combination of ATRA or 1,25-dihydroxyvitamin D 3 . Treatment of HL-60 cells with zero to 100 μg/ml of KV-1 for 72 h induced a small increase in cell differentiation. Surprisingly, a synergistic induction of differentiation was observed when the HL-60 cells were treated with ATRA or 1,25-(OH) 2 D 3 and the extract. The inhibitors of protein kinase C (PKC) (· and ßI) and extracellular signalregulated kinase (ERK), but not of phosphoinositide 3-kinase (PI3-K) and c-Jun N-terminal kinase (JNK) inhibited the HL-60 differentiation induced by the extract in combination of ATRA or 1,25-(OH) 2 D 3 , suggesting that PKC and ERK were involved in the cell differentiation enhancement by the extract. The results indicate that the acetone extract of D. kaki leaves has the ability to enhance HL-60 cell differentiation and suggest that it may be useful in acute promyelocytic leukemia therapy.

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“…It was reported previously that some triterpenoids (Chen et al , ; Yagi‐Chaves et al , ; He et al , ) and tricyclic diterpenoids (Tong et al , ) suppressed tyrosyl or serine/threonine phosphorylation of proteins and translocation to the plasma membrane of p47 phox , p67 phox and rac in parallel to the effect of stimulus‐induced superoxide generation. The present study investigated the effects of five secoiridoids isolated from the root of G .…”

Section: Introductionmentioning

confidence: 86%

Inhibitory Effects of Secoiridoids from the Roots of Gentiana straminea on Stimulus‐induced Superoxide Generation, Phosphorylation and Translocation of Cytosolic Compounds to Plasma Membrane in Human Neutrophils

Wei

Chen

et al. 2011

Phytotherapy Research

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Gentiana straminea Maxim. has been used widely as a traditional Chinese medicine for the treatment of rheumarthritis, icterepatitis, constipation, pain and hypertension. Five secoiridoids, gentiopicroside (GTP), 6'-O-(2-hydroxy-3-O-β-D-glucopyranosyl-benzoyl)-sweroside (HGBS), 6'-O-β-D-glucosylgentiopicroside (GGTP), sweroside (SW) and swertiamarin (STM) were isolated from the roots of G. straminea. The effect of these secoiridoids on stimulus-induced superoxide generation in human neutrophils was assayed by measuring the reduction of ferricytochrome c. Tyrosyl or serine/threonine phosphorylation of neutrophil proteins, and translocation of the cytosolic compounds to the cell membrane were also investigated using specific monoclonal antibodies. The five secoiridoids used in the present experiment suppressed N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced superoxide generation in a concentration dependent manner. GTP and HGBS also suppressed phorbol 12-myristate 13-acetate (PMA) and arachidonic acid (AA)-induced superoxide generation. However, the other three secoiridoids showed no effect on PMA- and AA-induced superoxide generation. fMLP-, PMA- and AA-induced tyrosyl or serine/threonine phosphorylation and translocation of the cytosolic proteins to the cell membrane were suppressed in parallel with the suppression of the stimulus-induced superoxide generation.

show abstract

Effect of five flavonoid compounds isolated from leaves of Diospyros kaki on stimulus-induced superoxide generation and tyrosyl phosphorylation of proteins in human neutrophils

Cited by 29 publications

References 20 publications

Bioactive flavonoids and saponins from Climacoptera obtusifolia

Bioactive flavonoids and saponins from Climacoptera obtusifolia

Effects and action mechanism of Diospyros kaki on the differentiation of human leukemia HL-60 cells

Inhibitory Effects of Secoiridoids from the Roots of Gentiana straminea on Stimulus‐induced Superoxide Generation, Phosphorylation and Translocation of Cytosolic Compounds to Plasma Membrane in Human Neutrophils

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