Effect of fish oil and coconut oil diet on the LDL receptor activity of rat liver plasma membranes

Tripodi, Alberto; Loria, Paola; Dilengite, Michele A.; Carulli, Nicola

doi:10.1016/0005-2760(91)90086-w

Cited by 37 publications

(17 citation statements)

References 41 publications

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“…However, this was associated with a significant reduc tion (22%) in absolute amount of serum HDL level as well (Table 5), The two-way ANOVA indicates highly significant dose effect in reducing serum cholesterol and HDL (p<0.0002). In fact, the HDL-lowering effect of dietary fish oil or n-3 fatty acids has been reported in rats (43) (Table 6). It may be specu lated that this was a compensatory response to a subtle hemolysis, as evidenced by enlarged spleen (Table 4), and/or to a diminished intestinal iron absorption caused by high level ingestion of PUFA.…”

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confidence: 99%

Subchronic Toxicity of Fish Oil Concentrates in Male and Female Rats.

Rabbani

Alan

Chirtel

et al. 2001

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryThere are an overwhelming number of reports indicating the beneficial effects of fish oil supplements in human and animal nutrition. The purpose of this study, second in a series, was to evaluate the effects, particularly those that may be harmful, of high-dose, long-term consumption of fish oil concentrates (FOC) using male and female rats. One hun dred and twenty male and 120 female rats were gavaged daily with oils and oil mixtures in a volume equal to 0.5% body weight (5mL/kg/d) for 13 weeks. The administered oils were corn oil, pure menhaden oil (MO), pure MaxEPA_??_ fish oil or different mixtures of corn oil with MO. The stability and the homogeneity of the dosing solutions were tested under study conditions. The animals received isocaloric and isonitrogenous diets throughout. Food and pure water were supplied ad libitum. At the end of the in-life phase of the study, the animals were anaesthetized with CO2 and humanely killed by exsanguination. Blood and other tis sues were prepared for various clinical, histopathological and laboratory tests. Some benefi cial effects of FOC, such as reduction in total serum cholesterol, in rats were confirmed. However, we also observed a significant reduction in absolute amount of serum HDL and a significant increase in relative liver and spleen weights in both sexes with the high dose of FOC. High doses of FOC (5mL/kg/d) reduced serum iron and vitamin E concentrations. A reduction in osmotic fragility of RBC as well as an increase in RBC deformity were also ob served in rats treated with high doses of FOC. These rats showed a significant overall in crease in WBC count. We conclude that in rats, subchronic consumption of high levels of FOC can be beneficial but may also be harmful because of induction of clinical abnormalities including increased red cell deformity, increased relative liver and spleen weights, and re duced serum HDL, iron and vitamin E concentrations.

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confidence: 99%

Subchronic Toxicity of Fish Oil Concentrates in Male and Female Rats.

Rabbani

Alan

Chirtel

et al. 2001

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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“…2). In rats, the corresponding binding parameters were estimated to 4,56 g/ml and 454 ng/mg membrane protein respectively (Tripodi et al 1991). This difference in affinity between assay with whole cells and membranes, probably reflects differences in pH, salts or degree of nonspecific binding in the two experimental systems.…”

Section: Resultsmentioning

confidence: 99%

Interaction of low density lipoproteins with liver cells in rainbow trout

Gjøen

Berg

1993

Fish Physiol Biochem

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Liver is the main catabolic tissue for low density lipoprotein in rainbow trout (Gjøen and Berg 1992). We have investigated the interaction of LDL with isolated trout liver cells and liver membranes. (125)I-TC labelled trout LDL bound to isolated trout liver cells in a time dependent and saturable manner with an apparant Kd of 20.1 μg/ml, suggesting the existence of a specific binding site on the surface of these cells. The binding was Ca(2+) dependent assessed by the 50% reduction obtained by 5 mM EDTA. Saturable binding to isolated trout liver membranes could also be demonstrated, but with lower affinity as compared to intact cells. Degradation of (125)I-TC-LDL in hepatocytes was also saturable as degradation could be inhibited about 60% by a 100 fold surplus of unlabelled LDL. The rate of degradation increased with temperature up to 20°C. Both cell association (binding + uptake) and degradation were reduced down to 20% of control in the presence of microtubular and lysosomal inhibitors. Hepatic catabolism of trout LDL therefore seems to depend on receptormediated endocytosis, followed by lysosomal degradation.

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“…Kobatake et al suggested that fish oil rich in DHA has a more potent reducing effect on exogenous plasma cholesterol than oil rich in EPA (9). Compared to the effect of a coconut oil diet, the effect of a fish oil diet is associated with a higher binding affinity of LDL to liver plasma membranes (10). Further more, it has been reported that dietary fish oil increased biliary excretion of cholesterol compared to a corn oil diet (11).…”

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confidence: 99%

Effect of Docosahexaenoic Acid and Sardine Oil Diets on the Ultrastructure of Hepatocytes in Adult Mice.

Tamura

Suzuki

1995

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryThe influence of docosahexaenoic acid (DHA) on the ultrastructure of hepatocytes was studied. Adult male mice of Crj:CD (ICR) strain were fed a fat-free purified diet supplemented with 5% (by weight) of purified DHA, refined sardine oil, and palm oil. The mice were fed the DHA diet or the palm oil diet for 7 days, and the sardine oil diet or the palm oil diet for one month. There were significant ultrastructural changes in the hepatocytes between the mice fed palm oil diet and the animals fed DHA and sardine oil diets. Many lipid droplets in the tissues of mice fed the palm oil diet were observed. Few lipid droplets were contained in the hepatocytes from the mice fed DHA and sardine oil diets, but electron-dense bodies were found in their tissues. These electron dense bodies were mainly found near the region of the nucleus, blood sinusoids and bile canaliculi. These results suggest that the dense bodies found in the DHA and sardine oil diet groups may appear as a result of acceleration of lipid metabolism in the liver of mice.

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Effect of fish oil and coconut oil diet on the LDL receptor activity of rat liver plasma membranes

Cited by 37 publications

References 41 publications

Subchronic Toxicity of Fish Oil Concentrates in Male and Female Rats.

Subchronic Toxicity of Fish Oil Concentrates in Male and Female Rats.

Interaction of low density lipoproteins with liver cells in rainbow trout

Effect of Docosahexaenoic Acid and Sardine Oil Diets on the Ultrastructure of Hepatocytes in Adult Mice.

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