Effect of fish meal substitution with dried bovine hemoglobin on the growth, blood hematology, antioxidant activity and related genes expression, and tissue histoarchitecture of Nile tilapia (Oreochromis niloticus)

Ibrahim, Rowida E.; Amer, Shimaa A.; Shahin, Shimaa A.; Darwish, Mahmoud I.M.; Albogami, Sarah; Abdel-Warith, Abdel-Wahab A.; Younis, Elsayed M.; Abduljabbar, Maram H.; Davies, Simon J.; Attia, G.

doi:10.1016/j.aqrep.2022.101276

Cited by 15 publications

(7 citation statements)

References 39 publications

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“…Lipase is the fat-digesting in sh and is essential for the intestine's absorption of lipids (Zhang et al 2020). Consequently, activation of the enzyme activity leads to improved digestion (Ibrahim et al 2023;). Large molecules are broken down by digestive enzymes into smaller ones that the cells can use to produce energy or other biomolecules.…”

Section: Discussionmentioning

confidence: 99%

Potential role of dietary white poplar (Populus alba L.) in stimulating growth, digestion, and antioxidant/immune status of Nile tilapia (Oreochromis niloticus)

Ibrahim,

ElHady,

Ghetas

et al. 2024

Preprint

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The potential use of feed supplements as immune-stimulant and growth-promoting agents in fish diets has drawn a lot of attention. The current research investigated the effects of feeding Nile tilapia (Oreochromis niloticus) on white poplar (Populus alba L.) leaves powder (WP) as a dietary supplement on the growth, digestive functions, and immne-antioxidant parameters. In addition, the fish resistance against Aeromonas sobria challenge was investigated. For 60 days, fish (n = 160; 34.61 ± 0.16 g) were divided equally into four groups, each had four replicates. Fish were fed on diets supplemented with varying levels of WP; 0 g/kg (WP0), 2 g/kg (WP2), 4 g/kg (WP4), and 6 g/kg (WP6), where WP0 was the control diet. The results revealed that WP diets (2–6 g/kg level) substantially boosted the growth (final body weight, weight gain, and specific growth rate %, with improvement of the feed conversion ratio) of Nile tilapia. WP diets improved the amylase (4–6 g/kg level) and lipase (2–6 g/kg level) activity and the intestinal morphometric measures (2–6 g/kg level). WP diets increased the growth hormone (4–6 g/kg level) and reduced leptin hormone and glucose levels (2–6 g/kg level). The immune-antioxidant indices (total protein, albumin, globulin, complement 3, lysozymes, nitric oxide, total antioxidant capacity, glutathione peroxidase, and catalase) were significantly improved by WP diets (2–6 g/kg level). The survival % of fish was improved in a level dependent manner (2–6 g/kg level) compared to the control (WP0) during the A. sobria challenge. Noteworthy, WP diets especially at a level of 6g/kg could be used as a feed supplement for improving the health, growth, immune-antioxidant functions, and disease resistance of Nile tilapia.

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Section: Discussionmentioning

confidence: 99%

Potential role of dietary white poplar (Populus alba L.) in stimulating growth, digestion, and antioxidant/immune status of Nile tilapia (Oreochromis niloticus)

Ibrahim,

ElHady,

Ghetas

et al. 2024

Preprint

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“…For histological investigation, the AmScope digital camera MU1803‐HS coupled to the Ceti England microscope was used. For the intestinal histomorphology assay, using ImageJ software 1.36 (NIH), the intestinal villous width (VW), villous length (VL), the crypt of Lieberkühn length (CL), muscular coat thickness (MCT) and goblet cells count (GCC) in the intestinal sections were measured (Ibrahim et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

Using Azadirachta indica protein hydrolysate as a plant protein in Nile tilapia (Oreochromis niloticus) diet: Effects on the growth, economic efficiency, antioxidant‐immune response and resistance to Streptococcus agalactiae

Rahman

Amer

Behairy

et al. 2023

Animal Physiology Nutrition

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A feeding trial for 90 days was conducted on Nile tilapia (Oreochromis niloticus) (average weight: 25.50 ± 0.05 g) to evaluate the effect of dietary inclusion of Azadirachta indica seed protein hydrolysate (AIPH). The evaluation included the impact on the growth metrics, economic efficiency, antioxidant potential, hemato‐biochemical indices, immune response, and histological architectures. A total of 250 fish were randomly distributed in five treatments (n = 50) and received diets included with five levels of AIPH (%): 0 (control diet, AIPH0), 2 (AIPH2), 4 (AIPH4), 6 (AIPH6) or 8 (AIPH8), where AIPH partially replace fish meal by 0, 8.7%, 17.4%, 26.1%, and 34.8%, respectively. After the feeding trial, a pathogenic bacterium (Streptococcus agalactiae, 1.5 × 108 CFU/mL) was intraperitoneally injected into the fish and the survival rate was recorded. The results elucidated that AIPH‐included diets significantly (p < 0.05) enhanced the growth indices (final body weight, total feed intake, total body weight gain, and specific growth rate) and intestinal morpho‐metrics (villous width, length, muscular coat thickness, and goblet cells count) in comparison to the control diet, with the AIPH8 diet recording the highest values. Dietary AIPH inclusion significantly improved (p < 0.05) the economic efficacy indicated by reduced feed cost/kg gain and increased performance index. The fish fed on the AIPH diets had noticeably significantly higher (p < 0.05) protein profile variables (total proteins and globulin) and antioxidant capabilities (superoxide dismutase and total antioxidant capacity) than the AIPH0 group. The dietary inclusion of AIPH significantly (p < 0.05) boosted the haematological parameters (haemoglobin, packed cell volume %, and counts of red blood cells and white blood cells) and immune indices (serum bactericidal activity %, antiprotease activity, and immunoglobulin M level) in a concentration‐dependent manner. The blood glucose and malondialdehyde levels were significantly (p < 0.05) lowered by dietary AIPH (2%−8%). The albumin level and hepatorenal functioning parameters (aspartate aminotransferase, alanine aminotransferase, and creatinine) were not significantly (p > 0.05) altered by AIPH diets. Additionally, AIPH diets did not adversely alter the histology of the hepatic, renal or splenic tissues with moderately activated melano‐macrophage centres. The mortality rate among S. agalactiae‐infected fish declined as dietary AIPH levels rose, where the highest survival rate (86.67%) was found in the AIPH8 group (p < 0.05). Based on the broken line regression model, our study suggests using dietary AIPH at the optimal level of 6%. Overall, dietary AIPH inclusion enhanced the growth rate, economic efficiency, health status, and resistance of Nile tilapia to the S. agalactiae challenge. These beneficial impacts can help the aquaculture sector to be more sustainable.

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“…The excessive use of fishmeal in fish feed has resulted in elevated costs and is not sustainable for long-term aquaculture production. Consequently, the quest for new, alternative, and efficient fishmeal substitutes has become increasingly urgent 2 , 3 . Plant proteins have been extensively studied as fishmeal substitutes in aquafeeds, including soybean meal 4 – 7 , rapeseed meal 8 (Cheng et al, 2010), cottonseed meal 9 , and peanut meal 10 .…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, the quest for new, alternative, and efficient fishmeal substitutes has become increasingly urgent 2 , 3 . Plant proteins have been extensively studied as fishmeal substitutes in aquafeeds, including soybean meal 4 – 7 , rapeseed meal 8 (Cheng et al, 2010), cottonseed meal 9 , and peanut meal 10 . However, plant proteins have challenges such as the presence of anti-nutritional factors, low feed availability, and an unbalanced amino acid profile, which negatively impacting various fish species 11 .…”

Section: Introductionmentioning

confidence: 99%

Significance of dietary quinoa husk (Chenopodium quinoa) in gene regulation for stress mitigation in fish

Kumar,

Thorat,

Pradhan

et al. 2024

Sci Rep

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The persistent challenges posed by pollution and climate change are significant factors disrupting ecosystems, particularly aquatic environments. Numerous contaminants found in aquatic systems, such as ammonia and metal toxicity, play a crucial role in adversely affecting aquaculture production. Against this backdrop, fish feed was developed using quinoa husk (the byproduct of quinoa) as a substitute for fish meal. Six isonitrogenous diets (30%) and isocaloric diets were formulated by replacing fish meal with quinoa husk at varying percentages: 0% quinoa (control), 15, 20, 25, 30 and 35%. An experiment was conducted to explore the potential of quinoa husk in replacing fish meal and assess its ability to mitigate ammonia and arsenic toxicity as well as high-temperature stress in Pangasianodon hypophthalmus. The formulated feed was also examined for gene regulation related to antioxidative status, immunity, stress proteins, growth regulation, and stress markers. The gene regulation of sod, cat, and gpx in the liver was notably upregulated under concurrent exposure to ammonia, arsenic, and high-temperature (NH3 + As + T) stress. However, quinoa husk at 25% downregulated sod, cat, and gpx expression compared to the control group. Furthermore, genes associated with stress proteins HSP70 and DNA damage-inducible protein (DDIP) were significantly upregulated in response to stressors (NH3 + As + T), but quinoa husk at 25% considerably downregulated HSP70 and DDIP to mitigate the impact of stressors. Growth-responsive genes such as myostatin (MYST) and somatostatin (SMT) were remarkably downregulated, whereas growth hormone receptor (GHR1 and GHRβ), insulin-like growth factors (IGF1X, IGF2X), and growth hormone gene were significantly upregulated with quinoa husk at 25%. The gene expression of apoptosis (Caspase 3a and Caspase 3b) and nitric oxide synthase (iNOS) were also noticeably downregulated with quinoa husk (25%) reared under stressful conditions. Immune-related gene expression, including immunoglobulin (Ig), toll-like receptor (TLR), tumor necrosis factor (TNFα), and interleukin (IL), strengthened fish immunity with quinoa husk feed. The results revealed that replacing 25% of fish meal with quinoa husk could improve the gene regulation of P. hypophthalmus involved in mitigating ammonia, arsenic, and high-temperature stress in fish.

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Effect of fish meal substitution with dried bovine hemoglobin on the growth, blood hematology, antioxidant activity and related genes expression, and tissue histoarchitecture of Nile tilapia (Oreochromis niloticus)

Cited by 15 publications

References 39 publications

Potential role of dietary white poplar (Populus alba L.) in stimulating growth, digestion, and antioxidant/immune status of Nile tilapia (Oreochromis niloticus)

Potential role of dietary white poplar (Populus alba L.) in stimulating growth, digestion, and antioxidant/immune status of Nile tilapia (Oreochromis niloticus)

Using Azadirachta indica protein hydrolysate as a plant protein in Nile tilapia (Oreochromis niloticus) diet: Effects on the growth, economic efficiency, antioxidant‐immune response and resistance to Streptococcus agalactiae

Significance of dietary quinoa husk (Chenopodium quinoa) in gene regulation for stress mitigation in fish

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