Effect of fish meal replacement by plant protein blend on amino acid concentration, transportation and metabolism in juvenile turbot (<i>Scophthalmus maximus</i>L.)

Xu, Dan; He, Gang; Mai, Kangsen; Wang, Qingchao; Li, M.; Zhou, Huihui; Xu, Wei; Song, Fei

doi:10.1111/anu.12486

Cited by 40 publications

(26 citation statements)

References 41 publications

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“…Fish fed the LS diet had decreased mRNA expression of SLC15A2, but not to the level of fish fed the LC diet. These results were in accordance with studies in blunt snout bream (Megalobrama amblycephala; Ahmed et al, 2019) and turbot (Scophthalmus maximus L.; Xu et al, 2017). The SLC7A5, SLC7A8, and SLC38A2, acting as the intermediates to regulate FAA transportation, were also identified in fish species (Xu et al, 2016(Xu et al, , 2017.…”

Section: Discussionsupporting

confidence: 90%

Dietary Lysine Regulates Body Growth Performance via the Nutrient-Sensing Signaling Pathways in Largemouth Bass (Micropterus salmoides)

Wang

Chi

et al. 2020

Front. Mar. Sci.

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Lysine is known to be a functional nutrient from yeast to human beings. However, the mechanisms for lysine to regulate body growth and metabolism in fish species are far from fully illuminated. In order to elucidate the molecular effects of lysine on growth performance and metabolism, largemouth bass (Micropterus salmoides), which is extremely sensitive to protein composition, was chosen as the experimental model. Juvenile largemouth bass (4.16 ± 0.02 g) were fed with the lysine control diet (LC diet), the lysine supplementation diet (LS diet), and the low lysine diet (LL diet) for 8 weeks. The results showed that the LS diet group significantly increased fish growth, feed efficiency, and nutrient retention compared with those of the LL diet group in largemouth bass. Moreover, compared to the LL diet, lysine supplementation also elevated the plasma total protein, total EAA, total AA, and some individual AA concentrations. Mechanistically, lysine supplementation had a significant effect to decrease the mRNA expression levels of peptide and AA transporters induced by lysine restriction. Lysine supplementation also had an important impact on regulating the target of rapamycin (TOR) signaling pathway. More importantly, the key regulators in the amino acid response (AAR) signaling pathway were also down-regulated by lysine supplementation. Our results provide a clear elucidation of how dietary lysine affected growth performance, physiological and biochemical responses, and signaling responses and represent a sound foundation for using lysine to improve the nutrient utilization of poultry by-product meal in largemouth bass.

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Section: Discussionsupporting

confidence: 90%

Dietary Lysine Regulates Body Growth Performance via the Nutrient-Sensing Signaling Pathways in Largemouth Bass (Micropterus salmoides)

Wang

Chi

et al. 2020

Front. Mar. Sci.

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“…PAT1 (SLC36A1) was found to mediate the transportation of amino acids such as proline ( 95 ) and act as an amino acid transceptor for the activation of TOR signaling in several cell lines ( 96 ). In addition, the major neutral amino acid transporter b 0 at1 ( slc6a19 ) ( 97 ) and cationic amino acid transporter b 0,+ at ( slc7a9 ) ( 98 ) were identified in turbot muscle in our previous study ( 99 ) and were found to be upregulated in the present study (Figure 5 D). These data suggested that the intracellular amino acid availabilities, activation of TOR signaling, and downstream anabolic processes were tightly coordinated and regulated.…”

Section: Discussionsupporting

confidence: 70%

The Mitotic and Metabolic Effects of Phosphatidic Acid in the Primary Muscle Cells of Turbot (Scophthalmus maximus)

Wang

Zhou

et al. 2018

Front. Endocrinol.

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Searching for nutraceuticals and understanding the underlying mechanism that promote fish growth is at high demand for aquaculture industry. In this study, the modulatory effects of soy phosphatidic acids (PA) on cell proliferation, nutrient sensing, and metabolic pathways were systematically examined in primary muscle cells of turbot (Scophthalmus maximus). PA was found to stimulate cell proliferation and promote G1/S phase transition through activation of target of rapamycin signaling pathway. The expression of myogenic regulatory factors, including myoD and follistatin, was upregulated, while that of myogenin and myostatin was downregulated by PA. Furthermore, PA increased intracellular free amino acid levels and enhanced protein synthesis, lipogenesis, and glycolysis, while suppressed amino acid degradation and lipolysis. PA also was found to increased cellular energy production through stimulated tricarboxylic acid cycle and oxidative phosphorylation. Our results identified PA as a potential nutraceutical that stimulates muscle cell proliferation and anabolism in fish.

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“…Likewise, Ostaszewska, Dabrowski, et al (), Ostaszewska, Kamaszewski, et al () reported an increased expression level of Pept1 in the digestive tract of common carp ( Cyprinus carpio ) and rainbow trout ( O. mykiss ) when fish were fed plant protein‐based diet supplemented with Lys–Gly dipeptide. Similarly, expression levels of Pept1 and Pept2 in the intestine were significantly increased against a high level of PP inclusion in the diet of juvenile turbot ( Scophthalmus maximus ; Xu et al, ).…”

Section: Discussionmentioning

confidence: 93%

Complete replacement of fish meal by plant protein ingredients with dietary essential amino acids supplementation for juvenile blunt snout bream ( Megalobrama amblycephala )

et al. 2018

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Six extruded diets were formulated with a graded level of fish meal (FM); diet 1 and diet 2 were formulated with 80 g/kg FM; diet 3 and diet 4 were formulated with 40 g/kg FM; and diet 5 and diet 6 were devoid of FM. Hence, diet 2, diet 4 and diet 6were supplemented with essential amino acids (EAAs). The diets were fed throughout an eight-week feeding trial. The results revealed that specific growth rate (SGR), feed conversion ratio (FCR), body weight gain (BWG) and protein efficiency ratio (PER) were influenced by FM reduction and improved by the addition of EAA (p < 0.05). A survival rate (SR), whole body content of protein, lipid, moisture and ash, and plasma total protein (TP), albumin (ALB), triglyceride (TG) and urea (UN) did not influence by dietary treatments (p > 0.05). Additionally, target of rapamycin (TOR) pathway did not influence by dietary treatments (p > 0.05). Expression levels of hepatic peptide transporter 1 (Pept1) and peptide transporter 2 (Pept2) decreased against FM reduction and improved significantly in the groups fed diet 4 and diet 6.In summary, the findings revealed that diet containing plant proteins mixture supplemented with EAA could totally replace FM in the practical diet of blunt snout bream. K E Y W O R D Sblunt snout bream (Megalobrama amblycephala), essential amino acids supplementation, extruded feed, fish meal reduction

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Effect of fish meal replacement by plant protein blend on amino acid concentration, transportation and metabolism in juvenile turbot (Scophthalmus maximusL.)

Cited by 40 publications

References 41 publications

Dietary Lysine Regulates Body Growth Performance via the Nutrient-Sensing Signaling Pathways in Largemouth Bass (Micropterus salmoides)

Dietary Lysine Regulates Body Growth Performance via the Nutrient-Sensing Signaling Pathways in Largemouth Bass (Micropterus salmoides)

The Mitotic and Metabolic Effects of Phosphatidic Acid in the Primary Muscle Cells of Turbot (Scophthalmus maximus)

Complete replacement of fish meal by plant protein ingredients with dietary essential amino acids supplementation for juvenile blunt snout bream ( Megalobrama amblycephala )

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