Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly(lactic acid) substrates

Badami, Anand S.; Kreke, Michelle; Thompson, M. Shane; Riffle, Judy S.; Goldstein, Aaron S.

doi:10.1016/j.biomaterials.2005.05.084

Cited by 563 publications

(443 citation statements)

References 46 publications

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“…Such a synergistic mechanism of cell interaction with nano-and microfibers, which is not entirely new, as recalled in the Introduction (i.e. similar observations have been reported for bimodal scaffolds formed of microfibers two orders of magnitude larger than ours [16,18]), improved colonization of the inner layers of the scaffolds. Fig.…”

Section: Cell Diffusion: Cell Morphology and Migration Mechanismssupporting

confidence: 68%

“…These fibers are exceedingly long (km range) compared with their diameters (x), which usually follow a unimodal statistical distribution. The mean and spread of such distributions can be controlled and tweaked via process parameters over a wide range, from a few nanometers to hundreds of microns [10][11][12][13][14][15][16][17][18][19][20][21][22][23]. The fiber diameter x is regarded as the prime controllable design parameter to steer scaffold performance in terms of cell response.…”

Section: Introductionmentioning

confidence: 99%

“…The fiber diameter x is regarded as the prime controllable design parameter to steer scaffold performance in terms of cell response. Reportedly, nanoscale fibers, in the absence of beads, were found to accelerate and improve cell adhesion and proliferation in the presence of growth factors with respect to the microscale counterpart -partly owing to the higher specific surface area providing a kinetic boost [18,21]. Nanofibers also appeared to sustain growth factor-induced stem cell differentiation [14,15,17,22,23].…”

Section: Introductionmentioning

confidence: 99%

“…This explains the pursuit for more advanced multiscale electrospun scaffolds. While some researchers have focused on unimodal fiber distributions spanning both the nanoscale and the microscale [21], others have begun to look for scaffolds featuring multimodal fiber distributions obtained by the combination of two or more fiber populations [16][17][18][19]. To date, this has typically been achieved by spinning microfibers and nanofibers alternately in a sequential fashion, to fabricate a layered composite that would inherit the advantages of both length scales and show enhanced cell functionality.…”

Section: Introductionmentioning

confidence: 99%

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Multiscale three-dimensional scaffolds for soft tissue engineering via multimodal electrospinning

et al. 2010

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a b s t r a c tA novel (scalable) electrospinning process was developed to fabricate bio-inspired multiscale threedimensional scaffolds endowed with a controlled multimodal distribution of fiber diameters and geared towards soft tissue engineering. The resulting materials finely mingle nano-and microscale fibers together, rather than simply juxtaposing them, as is commonly found in the literature. A detailed proof of concept study was conducted on a simpler bimodal poly(e-caprolactone) (PCL) scaffold with modes of fiber distribution at 600 nm and 3.3 lm. Three conventional unimodal scaffolds with mean diameters of 300 nm and 2.6 and 5.2 lm, respectively, were used as controls to evaluate the new materials. Characterization of the microstructure (i.e. porosity, fiber distribution and pore structure) and mechanical properties (i.e. stiffness, strength and failure mode) indicated that the multimodal scaffold had superior mechanical properties (Young's modulus $40 MPa and strength $1 MPa) in comparison with the controls, despite the large porosity ($90% on average). A biological assessment was conducted with bone marrow stromal cell type (mesenchymal stem cells, mTERT-MSCs). While the new material compared favorably with the controls with respect to cell viability (on the outer surface), it outperformed them in terms of cell colonization within the scaffold. The latter result, which could neither be practically achieved in the controls nor expected based on current models of pore size distribution, demonstrated the greater openness of the pore structure of the bimodal material, which remarkably did not come at the expense of its mechanical properties. Furthermore, nanofibers were seen to form a nanoweb bridging across neighboring microfibers, which boosted cell motility and survival. Lastly, standard adipogenic and osteogenic differentiation tests served to demonstrate that the new scaffold did not hinder the multilineage potential of stem cells.

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Section: Cell Diffusion: Cell Morphology and Migration Mechanismssupporting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Multiscale three-dimensional scaffolds for soft tissue engineering via multimodal electrospinning

et al. 2010

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“…Meanwhile, the surface chemistry and topography of materials play a very crucial role in altering cell behaviors at many stages of cell growth and development. [[qv: 1c]],3 Although the dimensions of mammalian cells are in the order of a few micrometers, cellular sensing of the external environment and interaction with biomaterials occurs at the nanometer level 4. Cell interactions with nanometric surfaces often result in a specific sequence of gene and protein regulations.…”

Section: Introductionmentioning

confidence: 99%

Virus Nanoparticles Mediated Osteogenic Differentiation of Bone Derived Mesenchymal Stem Cells

et al. 2015

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There are few methodologies that allow manipulating a biomaterial surface at nanometer scale, which controllably influence different cellular functions. In this study, virus nanoparticles with different structural features are selected to prepare 2D substrates with defined nanoscale topographies and the cellular responses are investigated. It is demonstrated that the viral nanoparticle based substrates could accelerate and enhance osteogenesis of bone derived mesenchymal stem cells as indicated by the upregulation of osteogenic markers, including bone morphogenetic protein‐2, osteocalcin, and osteopontin, at both gene and protein expression levels. Moreover, alkaline phosphatase activity and calcium mineralization, both indicators for a successful bone formation, are also increased in cells grown on these nanoscale possessed substrates. These discoveries and developments present a new paradigm for nanoscale engineering of a biomaterial surface.

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2007

Science and Technology of Polymer Nanofibers

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Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly(lactic acid) substrates

Cited by 563 publications

References 46 publications

Multiscale three-dimensional scaffolds for soft tissue engineering via multimodal electrospinning

Multiscale three-dimensional scaffolds for soft tissue engineering via multimodal electrospinning

Virus Nanoparticles Mediated Osteogenic Differentiation of Bone Derived Mesenchymal Stem Cells

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