Effect of fetal mass, number and stage of gestation on pregnancy-specific protein B concentrations in the bovine

Patel, Osman V.; Domeki, Ikuo; Sasaki, Nobuo; Takahashi, Toshio; Hirako, Makoto; Sasser, R.G.; Humblot, P.

doi:10.1016/0093-691x(95)00268-d

Cited by 31 publications

(34 citation statements)

References 26 publications

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“…In ruminants, there is evidence that PSPB and PSP-60 are related to the stage of gestation and fetal number and are indicators of fetoplacental viability (7,(11)(12)(13). However, there is only a single report in the literature on the peripheral concentrations of bPAG throughout gestation in the cow (9), and no data on the effect of conceptus number on the peripheral concentration of this protein in any species.…”

Section: Introductionmentioning

confidence: 89%

Plasma bovine pregnancy-associated glycoprotein concentrations throughout gestation in relationship to fetal number in the cow

Patel

Sulon

Beckers

et al. 1997

European Journal of Endocrinology

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This study characterized the peripheral plasma bovine pregnancy-associated glycoprotein (bPAG) profile throughout gestation and examined the effect of stage of gestation and fetal number on this profile in Holstein cows after non-surgical embryo transfer. Cows (n ¼ 12) were divided into three groups: group 1 ¼ normal singleton pregnancies (n ¼ 5); group 2 ¼ normal twin pregnancies (n ¼ 5); group 3 ¼ abnormal twin pregnancies (n ¼ 2). Blood was collected about every third day from day 0 (defined as the first day of standing estrus), then daily for the last 10 days of gestation, and sampling was stopped one day postpartum. The time-related changes in plasma bPAG concentrations were significantly (P < 0:01) affected by the stage of gestation and fetal number (P < 0:01), except during the last 10 days of gestation. In both normal pregnancy groups, bPAG concentration increased rapidly during the first trimester (0.5Ϯ0.1 to 14.6Ϯ1.7 ng/ml and 1.0Ϯ0.6 to 21.8Ϯ4.8 ng/ml, in singleton and twin-bearing groups respectively), then progressively between days 160 and 20 prepartum (31.6Ϯ6.2 to 114.3Ϯ31.3 ng/ml and 41.6Ϯ7.4 to 155.8Ϯ36.6 ng/ml in singleton and twin-bearing cows respectively). The mean concentration between days 20 and 10 prepartum approximately tripled (P < 0:001) in both these groups of cows (114.3Ϯ31.1 to 493.0Ϯ75.3 ng/ml and 155.8Ϯ36.6 to 409.3Ϯ114.7 ng/ml in singleton and twin-bearing cows respectively), but between days 10 prepartum and parturition the values increased about threefold (P < 0:01) in the singleton group (493.0Ϯ75.3 to 1352.8Ϯ286.5 ng/ml) and fivefold (P < 0:001) in the twin-bearing group (409.3Ϯ114.7 to 2154.0Ϯ505.7 ng/ml). The two cows in group 3 that gave birth prematurely to a stillborn calf or to a schistosomus reflexus calf exhibited an aberrant bPAG profile. Our results indicate that peripheral bPAG concentrations are correlated to the stage of gestation and fetal number, and that the profile of the peripheral plasma concentrations provides a useful indication of the feto-placental status.

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Section: Introductionmentioning

confidence: 89%

Plasma bovine pregnancy-associated glycoprotein concentrations throughout gestation in relationship to fetal number in the cow

Patel

Sulon

Beckers

et al. 1997

European Journal of Endocrinology

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“…The amounts of placental products in maternal circulation appear to be associated with placental and foetal masses, including foetal number and neonatal birth weight, and with pregnancy normalcy (Byatt et al 1992a, Patel et al 1995, Vasquez et al 1995 2001, Gootwine 2004). In this study, concentrations of maternal and foetal plasma bPL followed similar trends reported by Hossner et al (1997), Holland et al (1997) and Patel et al (1996), but concentrations of bPL and bPSPB in foetal plasma were correlated with conceptus physical traits and with one another in plasma and fluids.…”

Section: Discussionmentioning

confidence: 99%

“…The amounts of BC products in maternal circulation appear to be associated with placental mass, foetal number and neonatal birth weight, and have been postulated to be an index for conceptus viability and pregnancy normalcy in cattle (Byatt et al 1992a, Patel et al 1995, Vasquez et al 1995, Szenci et al 1998, Tefera et al 2001, Ravelich et al 2004. Associations between abnormal placental and foetal development after in vitro embryo manipulations have been suggested to play a key role in the occurrence of high birth weights.…”

Section: Introductionmentioning

confidence: 99%

“…Despite the unknown function and control of PSPB secretion, many potential biological functions have been suggested based on in vivo and in vitro effects, including prostaglandin release by the endometrium, implantation, immunotolerance of the conceptus as a tissue alograft and uterine remodelling after parturition, among others (Del Vecchio et al 1990, Kiracofe et al 1993, Austin et al 1999, Tefera et al 2001. Both PAG-1 and PSPB diagnostic assays have been developed for early plasma diagnosis of pregnancy in cattle and other ruminants (Sasser et al 1986, Zoli et al 1991, Green et al 2000.The amounts of BC products in maternal circulation appear to be associated with placental mass, foetal number and neonatal birth weight, and have been postulated to be an index for conceptus viability and pregnancy normalcy in cattle (Byatt et al 1992a, Patel et al 1995, Vasquez et al 1995, Szenci et al 1998, Tefera et al 2001, Ravelich et al 2004. Associations between abnormal placental and foetal development after in vitro embryo manipulations have been suggested to play a key role in the occurrence of high birth weights.…”

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confidence: 99%

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Expression profile and protein levels of placental products as indirect measures of placental function in in vitro-derived bovine pregnancies

Bertolini¹,

Wallace²,

Anderson³

2006

Reproduction

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Bovine conceptus development and its association with placental proteins present in maternal, foetal and neonatal plasma and foetal (amniotic and allantoic) fluids were investigated in in vivo-and in vitro-produced (IVP) concepti and newborn calves. Females were superovulated to obtain control embryos, whereas IVP embryos were derived from established in vitro procedures. Pregnant animals were slaughtered on days 90 or 180 of gestation or allowed to develop to term for the assessment of physical traits. Foetal, maternal and neonatal blood and foetal fluids were collected for the determination of bovine placental lactogen (bPL) and bovine pregnancy-specific protein B (bPSPB) concentrations. Placental transcripts for bPL and bPSPB, determined by quantitative RT-PCR, were elevated in IVP placentomes. No major physical differences were observed between groups on day 90, but concentrations of bPL and bPSPB were higher in foetal plasma and allantoic fluid of IVP concepti in day 180 pregnancies, which were correlated with larger uterine and conceptus traits. Maternal concentrations of bPL in IVP pregnancies were lower than controls during the last 8 weeks of gestation, to become similar as parturition approached. Newborn IVP calves and foetal membranes were larger and displayed higher concentrations of plasma bPL than controls (10 and 60 min after birth). Our results indicated that differential patterns of secretion of bPL and bPSPB into the maternal and foetal systems occurred at distinct stages of gestation, and these were associated with altered conceptus development after in vitro embryo manipulations, indirectly demonstrating deviations in placental function in IVP pregnancies. Reproduction (2006) 131 163-173 IntroductionWith the onset of the trophoblast apposition to the endometrium during the process of bovine placenta formation and development, large granulated cells, the trophoblast binucleate cells (BCs), migrate from the trophectoderm to fuse with uterine epithelial cells (Wooding 1992), forming short-lived trinucleate cells. This phenomenon occurs throughout pregnancy in cattle, but the number and period of existence of trinucleate cells decline towards the end of gestation (Wooding 1992, Green et al. 2000. Binucleate cells comprise about 20% of the trophoblast cells and are responsible for hormones and bioactive products important for the regulation of foetal growth and development (Anthony et al. 1995, Green et al. 2000, Schlafer et al. 2000. The contents of the membranebounded secretory granules of BCs are released by exocytosis towards the maternal capillary bed, reaching the maternal blood system (Wooding 1992). Many proteins are produced by the BCs, including placental lactogen (PL) and a range of glycoproteins (Wooding 1992), such as pregnancy-specific protein B (PSPB) (Butler et al. 1982).PL lactogen is a member of the growth hormone (GH)/prolactin (PRL) gene family synthesized and stored in secretory granules of the trophoblast BCs (Byatt et al. 1992a, Wooding 1992, Gootwine 2004. Although ...

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“…Multiple PAG genes have been cloned and identified in many domestic animals such as cattle, sheep [5], goat [11], pig [12], and wild ruminants' species [5]. Based on the evolutionary study and phylogenetic linkage bovine pregnancy associated glycoproteins family has been segregated as ancient (bovine PAG-2, bovine PAG-8) and modern (bovine PAG-1) [13][14][15].…”

Section: Genetics Research Internationalmentioning

confidence: 99%

Characterization and in silico Analysis of Pregnancy Associated Glycoprotein-1 Gene of Buffalo (Bubalus bubalis)

Adnot¹,

Singh²,

Agarwal³

et al. 2010

J. of Molecular Genetics

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Pregnancy-Associated Glycoproteins (PAGs) are trophoblastic proteins belonging to the Aspartic proteinase family secreted by different placental cells of many mammalian species. They play a pivotal role in placentogenesis, foetomaternal unit remodeling, and implantation. The identification of the genes encoding those proteins will be helpful to unravel the intricate embryogenomic functions during pregnancy establishment. Considering importance of these proteins, the present study was undertaken to characterize the pregnancy associated glycoprotein-1 gene of buffalo. An 1181 base pairs buffalo PregnancyAssociated Glycoprotein PAG-1 gene was PCR amplified from the RNA obtained from the fetal cotyledons. BLAST analysis of the buffalo PAG-1 sequence retrieved a total of 20 cattle, 5 goat, and 4 sheep PAG sequences, exhibiting more than 80% similarity. Buffalo PAG-1 gene contained an uninterrupted open reading frame of 1140 base pairs encoding 380 amino acids that possess a 15 amino acid signal peptide and mature peptide of 365 amino acids. The phylogenetic study of the buffalo PAG-1 gene revealed buffalo PAG-1 is more related to cattle, goat, and sheep PAG-1 sequences. By this study characterization of buffalo PAG-1 gene and its evolutionary relationship was deduced for the first time.

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Effect of fetal mass, number and stage of gestation on pregnancy-specific protein B concentrations in the bovine

Cited by 31 publications

References 26 publications

Plasma bovine pregnancy-associated glycoprotein concentrations throughout gestation in relationship to fetal number in the cow

Plasma bovine pregnancy-associated glycoprotein concentrations throughout gestation in relationship to fetal number in the cow

Expression profile and protein levels of placental products as indirect measures of placental function in in vitro-derived bovine pregnancies

Characterization and in silico Analysis of Pregnancy Associated Glycoprotein-1 Gene of Buffalo (Bubalus bubalis)

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