NAWRATH ADAM, ELBL JAKUB, KINTL ANTONÍN, ZÁHORA JAROSLAV, SKLÁDANKA JIŘÍ: The effi ciency of nutrient utilization by permanent grassland in the Kameničky locality. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2013, LXI, No. 6, pp. 1799-1806 This work presents the analysis of the eff ect of grassland management on leaching of mineral nitrogen from rhizosphere and plant biomass production in area of our interest. The determined values show the infl uence of fertilization and species composition of permanent grassland on leaching of mineral nitrogen and biomass production. The values were determined during fi eld and laboratory experiments. The highest availability of N min in the fi eld experiment was detected in the variant N90+PK; the lowest availability of N min was recorded in the PK variant. The diff erence was statistically signifi cant (P < 0.05). In the pot experiment, the highest loss of N min was recorded in the variant with N180+PK (16.88 mg.dm −3