Effect of ferrous sulfate, sodium metabisulfite, and sodium pyruvate on survival of Campylobacter jejuni

Chou, Shu Pang; Dular, Ram; Kasatiya, S. S.

doi:10.1128/jcm.18.4.986-987.1983

Cited by 19 publications

(6 citation statements)

References 8 publications

(11 reference statements)

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“…Number: 41061-029). This was supplemented with ferrous sulphate/sodium metabisulphite/sodium pyruvate (FBP), an iron source and protectant against oxidative stress (Chou et al, 1983). A freshly prepared stock FBP solution [2.5% (w/v) each of ferrous sulphate, sodium metabisulphite and sodium pyruvate in water] was filter sterilized and added to the media at a dilution of 1:2000.…”

Section: Methodsmentioning

confidence: 99%

Amino acid‐dependent growth of Campylobacter jejuni: key roles for aspartase (AspA) under microaerobic and oxygen‐limited conditions and identification of AspB (Cj0762), essential for growth on glutamate

Guccione¹,

Leon-Kempis²,

Pearson³

et al. 2008

Molecular Microbiology

148

187

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SummaryAmino acids are key carbon and energy sources for the asaccharolytic food-borne human pathogen Campylobacter jejuni. During microaerobic growth in amino acid rich complex media, aspartate, glutamate, proline and serine are the only amino acids significantly utilized by strain NCTC 11168. The catabolism of aspartate and glutamate was investigated. An aspartase (aspA) mutant (unable to utilize any amino acid except serine) and a Cj0762c (aspB) mutant lacking aspartate:glutamate aminotransferase (unable to utilize glutamate), were severely growth impaired in complex media, and an aspA sdaA mutant (also lacking serine dehydratase) failed to grow in complex media unless supplemented with pyruvate and fumarate. Aspartase was shown by activity and proteomic analyses to be upregulated by oxygen limitation, and aspartate enhanced oxygen-limited growth of C. jejuni in an aspA-dependent manner. Stoichiometric aspartate uptake and succinate excretion involving the redundant DcuA and DcuB transporters indicated that in addition to a catabolic role, AspA can provide fumarate for respiration. Significantly, an aspA mutant of C. jejuni 81-176 was impaired in its ability to persist in the intestines of outbred chickens relative to the parent strain. Together, our data highlight the dual function of aspartase in C. jejuni and suggest a role during growth in the avian gut.

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Section: Methodsmentioning

confidence: 99%

Amino acid‐dependent growth of Campylobacter jejuni: key roles for aspartase (AspA) under microaerobic and oxygen‐limited conditions and identification of AspB (Cj0762), essential for growth on glutamate

Guccione¹,

Leon-Kempis²,

Pearson³

et al. 2008

Molecular Microbiology

148

187

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“…Multiple factors have been reported to affect C. jejuni survival in oxygen-rich conditions. For example, pyruvate protects C. jejuni from high oxygen stress in aerobic conditions ( Verhoeff-Bakkenes et al, 2008 ), and a combinational use of ferrous sulfate, sodium metabisulfite and sodium pyruvate in culture media increases the viability of C. jejuni ( Chou et al, 1983 ). In addition, metabolic commensalism with Pseudomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Impact of oxidative stress defense on bacterial survival and morphological change in Campylobacter jejuni under aerobic conditions

McMullen

Jeon

2015

Front. Microbiol.

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Campylobacter jejuni, a microaerophilic foodborne pathogen, inescapably faces high oxygen tension during its transmission to humans. Thus, the ability of C. jejuni to survive under oxygen-rich conditions may significantly impact C. jejuni viability in food and food safety as well. In this study, we investigated the impact of oxidative stress resistance on the survival of C. jejuni under aerobic conditions by examining three mutants defective in key antioxidant genes, including ahpC, katA, and sodB. All the three mutants exhibited growth reduction under aerobic conditions compared to the wild-type (WT), and the ahpC mutant showed the most significant growth defect. The CFU reduction in the mutants was recovered to the WT level by complementation. Higher levels of reactive oxygen species were accumulated in C. jejuni under aerobic conditions than microaerobic conditions, and supplementation of culture media with an antioxidant recovered the growth of C. jejuni. The levels of lipid peroxidation and protein oxidation were significantly increased in the mutants compared to WT. Additionally, the mutants exhibited different morphological changes under aerobic conditions. The ahpC and katA mutants developed coccoid morphology by aeration, whereas the sodB mutant established elongated cellular morphology. Compared to microaerobic conditions, interestingly, aerobic culture conditions substantially induced the formation of coccoidal cells, and antioxidant treatment reduced the emergence of coccoid forms under aerobic conditions. The ATP concentrations and PMA–qPCR analysis supported that oxidative stress is a factor that induces the development of a viable-but-non-culturable state in C. jejuni. The findings in this study clearly demonstrated that oxidative stress resistance plays an important role in the survival and morphological changes of C. jejuni under aerobic conditions.

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“…2 (6,19), brucella agar (1,5,21,23), Columbia blood agar base (7, 13), Mueller-Hinton agar (16,17), and thioglycolate agar medium (8). Common supplements include horse blood (5 to 7%) for use when trimethoprim is incorporated as a selective agent (4,6) or sheep blood (5 to 15%) (6,10,16,19,20) or FBP (a mixture of ferrous sulfate, sodium bisulfite, and sodium pyruvate), which is added to increase the aerotolerance of the organisms (7,11,12), or both. A combination of antibiotics is usually added to inhibit other bacteria present in clinical and environmental samples.…”

mentioning

confidence: 99%

Comparison of basal media for culturing Campylobacter jejuni and Campylobacter coli

Ng¹,

Stiles²,

Taylor³

1985

J Clin Microbiol

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Effect of ferrous sulfate, sodium metabisulfite, and sodium pyruvate on survival of Campylobacter jejuni

Cited by 19 publications

References 8 publications

Amino acid‐dependent growth of Campylobacter jejuni: key roles for aspartase (AspA) under microaerobic and oxygen‐limited conditions and identification of AspB (Cj0762), essential for growth on glutamate

Amino acid‐dependent growth of Campylobacter jejuni: key roles for aspartase (AspA) under microaerobic and oxygen‐limited conditions and identification of AspB (Cj0762), essential for growth on glutamate

Impact of oxidative stress defense on bacterial survival and morphological change in Campylobacter jejuni under aerobic conditions

Comparison of basal media for culturing Campylobacter jejuni and Campylobacter coli

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