Effect of fermentation temperature on hydrogen production from cow waste slurry by using anaerobic microflora within the slurry

Yokoyama, Hiroshi; Waki, Miyoko; Moriya, Naoko; Yasuda, Tomoko; Tanaka, Yasuo; Haga, Kiyonori

doi:10.1007/s00253-006-0647-4

Cited by 133 publications

(43 citation statements)

References 51 publications

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“…This variation is another important characteristic that could permit the coexistence of so close relatives. Temperature adaptability is likely the result of regulation of enzymatic activity, which might directly influence the efficiency of the biochemical pathways involved in Microcystis decomposition (Sridhar et al, 2000;Yokoyama et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…During the anaerobic incubation of the Microcystis slurry, Clostridium species appear to have the collaborative ability to hydrolyze initial substrates and produce low molecular weight intermediates, such as acetate and ethanol, and even directly generate biohydrogen gas (Chang et al, 2006;Yokoyama et al, 2007;Seedorf et al, 2008). The occurrence of these processes in the fermentation systems was confirmed by the presence of methanogenic Archaea (our unpublished data), which anaerobically utilize low molecular weight intermediates (for example, acetate or H 2 /CO 2 ) to produce CH 4 (Supplementary Figure S6).…”

Section: Discussionmentioning

confidence: 99%

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Novel Clostridium populations involved in the anaerobic degradation of Microcystis blooms

et al. 2010

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Understanding the microbial degradation of Microcystis biomass is crucial for determining the ecological consequences of Microcystis blooms in freshwater lakes. The purpose of this study was to identify bacteria involved in the anaerobic degradation of Microcystis blooms. Microcystis scum was anaerobically incubated for 90 days at three temperatures (15 1C, 25 1C and 35 1C). We used terminal restriction fragment length polymorphism (T-RFLP) analysis of bacterial 16S rRNA genes, followed by cloning and sequencing of selected samples, to reveal the community composition of bacteria and their dynamics during decomposition. Clostridium spp. were found to be the most dominant bacteria in the incubations, accounting for 72% of the sequenced clones. Eight new clusters or subclusters (designated CLOS.1-8) were identified in the Clostridium phylogenetic tree. The bacterial populations displayed distinct successions during Microcystis decomposition. Temperature had a strong effect on the dynamics of the bacterial populations. At 15 1C, the initial dominance of a 207-bp T-RF (Betaproteobacteria) was largely substituted by a 227-bp T-RF (Clostridium, new cluster CLOS.2) at 30 days. In contrast, at 25 1C and 35 1C, we observed an alternating succession of the 227-bp T-RF and a 231-bp T-RF (Clostridium, new cluster CLOS.1) that occurred more than four times; no one species dominated the flora for the entire experiment. Our study shows that novel Clostridium clusters and their diverse consortiums dominate the bacterial communities during anaerobic degradation of Microcystis, suggesting that these microbes' function in the degradation process.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Novel Clostridium populations involved in the anaerobic degradation of Microcystis blooms

et al. 2010

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“…However, comparatively little is known about the occurrence of anaerobic extreme thermophiles, with growth optima above 65 u C, in animal faeces. A study has reported the presence of an anaerobic extreme thermophile, Caldanaerobacter subterraneus, in cow faeces (Yokoyama et al, 2007a), implying that dormant extreme thermophiles might prevail in animal faeces. Many anaerobic thermophiles can degrade hemicellulosic material, the second most abundant component of plant fibre; however, the other extremely thermophilic hemicellulolytic bacteria were not isolated from animal faeces but from hot springs or oil-producing wells (Wagner & Wiegel, 2008).…”

mentioning

confidence: 99%

Caldicoprobacter oshimai gen. nov., sp. nov., an anaerobic, xylanolytic, extremely thermophilic bacterium isolated from sheep faeces, and proposal of Caldicoprobacteraceae fam. nov.

Yokoyama

Wagner

Wiegel

2010

International Journal of Systematic and Evolutionary Microbiology

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An obligately anaerobic, xylanolytic, extremely thermophilic bacterium, strain JW/HY-331T, was isolated from sheep faeces collected from a farm at the University of Georgia, USA. Cells of strain JW/HY-331T stained Gram-positive and were catalase-negative, non-motile rods. Single terminal endospores (0.4–0.6 μm in diameter) swelled the mother cell. Growth ranges were 44–77 °C (optimum 70 °C at pH70 °C 7.2) and pH70 °C 5.9–8.6 (optimum 7.2 at 70 °C). Salt tolerance was 0–2.0 % (w/v) NaCl. No growth was observed at or below 42 °C or at or above 79 °C or at pH70 °C 5.7 and below or 8.9 and above. In the presence of 0.3 % yeast extract and 0.1 % tryptone, strain JW/HY-331T utilized xylose, glucose, galactose, cellobiose, raffinose and xylan as carbon and energy sources, but not dextran, soluble potato starch, CM-cellulose, cellulose powder, casein or Casamino acids. Fermentation products from glucose were lactate, acetate, ethanol, CO2 and H2. The G+C content of the genomic DNA was 45.4 mol% (HPLC). Major cellular fatty acids were iso-C17 : 0, iso-C15 : 0 and anteiso-C17 : 0. No respiratory quinones were detected. The cell-wall structure was a single layer (Gram-type positive) of the peptidoglycan type A1γ; the cell-wall sugars were galactose and mannose. Based on 16S rRNA gene sequence analysis, ‘Catabacter hongkongensis’ HKU16 (85.4 % similarity), Caloramator fervidus ATCC 43204T (84.2 %) and Caloranaerobacter azorensis MV1087T (83.4 %) were the closest relatives, but they were only distantly related to strain JW/HY-331T. On the basis of physiological, chemotaxonomic and phylogenetic data, isolate JW/HY-331T (=DSM 21659T =ATCC BAA-1711T) is proposed as the type strain of Caldicoprobacter oshimai gen. nov., sp. nov., placed in Caldicoprobacteraceae fam. nov. within the order Clostridiales of the phylum Firmicutes.

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“…With the inoculum pretreatment (boiling at 100 C for 1 h) and the acclimation process (55 C for 3 d) for the seed sludge, they found that dark fermentation of acid-hydrolyzed ground wheat was more beneficial under thermophilic condition (55 C) than mesophilic condition (37 C). Actually, by the inoculation of mesophilically and thermophilically digested sludge, thermophilic acidogenic sludge, cow waste slurry, etc., peak H 2 yields can be commonly achieved under thermophilic environments (55e60 C) with other complex wastes as the substrate [34,35]. In our view, better H 2 -producing performances under thermophilic conditions can be attributed to selective enrichment of some efficient H 2 -producing thermophiles, which are capable of producing more H 2 by utilizing complex substrate components (to be illustrated in section 3.4).…”

Section: Biogas and H 2 Productionmentioning

confidence: 95%

Fermentative hydrogen production from corn stover hydrolyzate by two typical seed sludges: Effect of temperature

Zhang

Ren

Wang

2015

International Journal of Hydrogen Energy

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Temperature Seed sludgeCorn stover hydrolyzate Denaturing gradient gel electropho- resis (DGGE)Microbial diversity analysis a b s t r a c tThe temperature effect on fermentative hydrogen (H 2 ) production from corn stover hydrolyzate was investigated under mesophilic (37 and 30 C), thermophilic (55 C), and extreme thermophilic (70 C) conditions by using two typical seed sludges (activated sludge and anaerobic granular sludge). Among various temperatures, the fermentation at 55 C reached the optimal H 2 production with the values of 6.08 mmol-H 2 /g-utilized sugar for activated sludge and 7.74 mmol-H 2 /g-utilized sugar for anaerobic granular sludge, respectively. For the two seed sludges, the effectiveness of fermentation temperature on H 2 production both followed the order as 55 C > 70 C > 37 C z 30 C. The soluble metabolites composition at 55 C showed the highest acetate and butyrate concentrations, as well as the minimum ethanol production, coinciding with better H 2 -producing performances in these cases. Microbial community analysis indicated that microbial community diversity significantly decreased with increased fermentation temperature. Facultative anaerobes, such as Enterobacter spp., Klebsiella spp., and Citrobacter spp., were dominant in microbial community of the two seed sludges. As efficient H 2 producers, Bacillus sp. AB5283 in activated sludge and Thermoanaerobacterium sp. PO-2009 in anaerobic granular sludge might be mainly responsible for high H 2 yields under thermophilic condition.

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Effect of fermentation temperature on hydrogen production from cow waste slurry by using anaerobic microflora within the slurry

Cited by 133 publications

References 51 publications

Novel Clostridium populations involved in the anaerobic degradation of Microcystis blooms

Novel Clostridium populations involved in the anaerobic degradation of Microcystis blooms

Caldicoprobacter oshimai gen. nov., sp. nov., an anaerobic, xylanolytic, extremely thermophilic bacterium isolated from sheep faeces, and proposal of Caldicoprobacteraceae fam. nov.

Fermentative hydrogen production from corn stover hydrolyzate by two typical seed sludges: Effect of temperature

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