Biodiesel production from plant seed oil and animal fat is not a new technologies, though recently searching for alternative renewable sources of fuel is receiving much attention due to global energy demand and increase in environmental pollution. Currently biodiesel is largely produced from edible oil feedstock which may not be sustainable in the longer term due to its competition with food, thus lead to a search for not edible oil feedstock for the production of green fuel. In view of this, homogeneous transesterification of Lageneraria sinceraria seed oil has been carried out using NaOH catalyst at 65 ͦ C with ethanol which produced a good biodiesel yield of 78% with HHV of 36.34 (MJ/Kg), 0.02% low total water and sediment level, 0.80g/cm 3 density, 0.82 g/cm 3 specific gravity, 27.20 g/cm 3 API gravity, 0.44 mg NaOH/g Acid number and 144 o C Flash point. The ethyl ester biodiesel produced, therefore, promises to be a viable source of energy for future use.