Effect of feeding Lactobacillus plantarum P-8 on the faecal microbiota of broiler chickens exposed to lincomycin

Gao, Pengfei; Hou, Qiangchuan; Kwok, Lai‐Yu; Huo, Dongxue; Feng, Shuzhen; Zhang, Heping

doi:10.1016/j.scib.2017.01.001

Cited by 28 publications

(22 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, HMs may either promote or inhibit the gut microbiota. Similarly, some probiotics, such as Lactobacillus plantarum , have been shown to modulate fecal parameters in chickens (Gao et al 2017 ), and Lactobacillus johnsonii has been shown to improve the gut microbiota in broiler chickens (Wang et al 2017a ). In our study, the addition of single Astragalus also modulated fecal microbiota.…”

Section: Discussionmentioning

confidence: 99%

“…However, the accumulation of antibiotic residues in chickens and eggs, and the subsequent prevalence of drug-resistant pathogens have received attention worldwide (Wang et al 2017b ). The use of antibiotics can result in gut dysbiosis, diarrhea, and host immune dysregulation (Willing et al 2011 ), which results in reduced growth and production (Gao et al 2017 ). Recent studies have shown that the composition of the gut microbiota affects various physiological functions of the host, such as nutrient utilization, gut epithelium nourishment, and the development and activity of the gut immune system (Ismail et al 2009 and Hill et al 2010 ).…”

Section: Introductionmentioning

confidence: 99%

“…The major phyla in fecal samples of laying hens include Firmicutes (58.8%), Bacteroidetes (22.1%), Proteobacteria (16.9%), Actinobacteria (0.6%), and Fusobacteria (1.4%) (Videnska et al 2014 ). Some functional feed additives, such as prebiotics (Rastall and Gibson 2015 ), probiotics (Gao et al 2017 ), phytase (Borda-Molina et al 2016 ), and fermented Ginkgo leaves (Zhang et al 2015 ), are reported to maintain microbial populations and support the health of the host. NGS has facilitated in-depth studies on the effect of HM on gut microbiota.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Astragalus affects fecal microbial composition of young hens as determined by 16S rRNA sequencing

et al. 2018

View full text Add to dashboard Cite

The gut microbiota play important roles in the degradation of chemical compounds of herbal medicines (HMs). However, little information regarding the interplay between HMs and the gut microbiota is available. Thus, the aim of this study was to investigate the composition of the fecal microbiota of young (age, 11 weeks) hens fed a conventional diet containing a crude Astragalus (0.5%) additive for 21 days (group A) vs. controls (group B) that were fed only conventional feed. The fecal contents of 14-week-old hens were collected for DNA extraction, and then the V3 and V4 hyper-variable regions of the 16S rRNA gene were amplified and analyzed using high-throughput sequencing technology. A distinctive difference in microbial diversity was observed between the two groups. The microbial composition of hens fed a diet supplemented with Astragalus was greater than that of the control group. At the genus level, Lactobacillus was more abundant in group A than group B (p < 0.05). Importantly, this study is the first to report the observation of a novel Romboutsia sp. in the feces of hens. However, Romboutsia was less abundant in group A than group B (17.94 vs. 33.98%, respectively, p < 0.05). The microbial community differed significantly between the two groups at the genus level, suggesting that Astragalus modulates the composition of the fecal microbiota. Based on these differences, these findings provide fresh insights into the application of Astragalus in the poultry industry, as well as a better understanding of the interplay between HMs and the gut microbiota.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Astragalus affects fecal microbial composition of young hens as determined by 16S rRNA sequencing

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The use of a high percentage of animal protein is avoided in chicken diets because it increases the abundance of Clostridium perfringens in the GIT which is a predisposing factor for necrotic enteritis in chickens [ 60 ]. The ban of antibiotics as growth promoters by the European Union and its potential restriction in other countries [ 61 ] intensified the search for alternatives to improve growth performance and to avoid a raise in animal diseases such as necrotic enteritis, gut dysbiosis, diarrhea, loss of appetite and dysregulation of the immune system [ 62 ].…”

Section: Chicken Feeding and Its Influence On The Microbiotamentioning

confidence: 99%

“…The use of the probiotic Lactobacillus plantarum P-8 in broiler diets enhances the immune response, weight gain, feed efficiency and feed intake. Moreover, metabolic activity and nutrient utilization are improved and furthermore, the fecal microbial composition is modulated [ 62 ]. Enterococcus faecium supplementation (0.5% of the total diet) reduces the microbial counts of Salmonella and increases body weight gain and breast muscle yield [ 85 ].…”

Section: Chicken Feeding and Its Influence On The Microbiotamentioning

confidence: 99%

Current Perspectives of the Chicken Gastrointestinal Tract and Its Microbiome

Borda-Molina

Seifert

Camarinha‐Silva

2018

Computational and Structural Biotechnology Journal

213

168

View full text Add to dashboard Cite

The microbial communities inhabiting the gastrointestinal tract (GIT) of chickens are essential for the gut homeostasis, the host metabolism and affect the animals' physiology and health. They play an important role in nutrient digestion, pathogen inhibition and interact with the gut-associated immune system.Throughout the last years high-throughput sequencing technologies have been used to analyze the bacterial communities that colonize the different sections of chickens' gut. The most common methodologies are targeted amplicon sequencing followed by metagenome shotgun sequencing as well as metaproteomics aiming at a broad range of topics such as dietary effects, animal diseases, bird performance and host genetics. However, the respective analyses are still at the beginning and currently there is a lack of information in regard to the activity and functional characterization of the gut microbial communities. In the future, the use of multi-omics approaches may enhance research related to chicken production, animal and also public health. Furthermore, combinations with other disciplines such as genomics, immunology and physiology may have the potential to elucidate the definition of a “healthy” gut microbiota.

show abstract

Characterization of bacterial community and flavor differences of different types of Douchi

Wang

Xiang

Zhang

et al. 2021

Food Science & Nutrition

Self Cite

View full text Add to dashboard Cite

According to the appearance and technology, traditional fermented Douchi can be divided into dried Douchi and wet Douchi. However, there are few reports on the difference of bacterial community structure between them or the influence of bacterial community on product flavor. In this study, high‐throughput sequencing technology and electronic nose were used to measure the bacterial diversity and flavor of 40 Douchi samples, and the correlation between them was explored by multivariate statistical means combined with COG database. Results showed that the cumulative average relative abundance of Firmicutes and Proteobacteria in the samples was as high as 95.93%, and the former was the core bacteria phylum. On the whole, the dominant bacteria in Douchi were Bacillus (50.67%), Staphylococcus (14.07%), Enterococcus (2.54%), Proteus (1.61%), Brevibacillus (1.46%), Providencia (1.26%), Weissella (1.24%), and Ureibacillus (1.19%). LEfSe analysis indicated that Bacillus can be used as a biomarker in dried fermented soybeans. Meanwhile, dried samples contained more intensive aromatic substances, but were significantly lower in W6S (selectivity to hydrogen) and W3S (methane‐aliph) compared with the wet samples. Aneurinibacillus and Brevibacillus were helpful to the formation of aromatic flavor in Douchi, but Vagococcus and Corynebacterium were the opposite. Gene and microbial phenotypic prediction showed that microorganisms in dried Douchi use protein more efficiently, while in wet Douchi, microbial energy metabolism was more vigorous. The pathogenic potential of microorganisms in dried samples was higher than that in wet. This study can sound the alarm for improving the safety of home‐brewed Douchi and provide guidance for the subsequent screening of strains that enhance the flavor of fermented soybeans.

show abstract

Effect of feeding Lactobacillus plantarum P-8 on the faecal microbiota of broiler chickens exposed to lincomycin

Cited by 28 publications

References 45 publications

Astragalus affects fecal microbial composition of young hens as determined by 16S rRNA sequencing

Astragalus affects fecal microbial composition of young hens as determined by 16S rRNA sequencing

Current Perspectives of the Chicken Gastrointestinal Tract and Its Microbiome

Characterization of bacterial community and flavor differences of different types of Douchi

Contact Info

Product

Resources

About