Effect of feeding Bacillus subtilis natto fermentation product on milk production and composition, blood metabolites and rumen fermentation in early lactation dairy cows

Abstract

This experiment was conducted to determine the effect of Bacillus subtilis natto fermentation product supplementation on blood metabolites, rumen fermentation and milk production and composition in early lactation dairy cows. Thirty-six multiparous Holstein cows (DIM = 29 ± 6 days, parity = 2.8 ± 1.1) were blocked by DIM and parity and then randomly assigned to three treatments (12 per treatment) in a 9-week trial. Cows in control, DFM1 and DFM2 were fed TMR diets supplemented with 0, 6 and 12 g of B. subtilis natto solid-state fermentation product per day per cow respectively. Plasma non-esterified fatty acids were lower (p = 0.03) in DFM1 and DFM2 compared with control cows (633 and 639 vs. 685 μm). Ruminal propionate increased (23.9 vs. 26.3 and 26.9/100 mol, control vs. DFM1 and DFM2 respectively) and acetate decreased (64.2 vs. 62.7 and 62.1/100 mol, control vs. DFM1 and DFM2 respectively) with increasing B. subtilis natto fermentation product supplementation. DMI of the cows in three treatments was not affected by B. subtilis natto fermentation product supplementation, but milk yield was 3.1 and 3.2 kg/day higher for DFM1 and DFM2 than that for control cows on average across the 9-week trial, and significant differences were observed during weeks 5-9 of the trial, which resulted in 9.5% and 11.7% increase in feed efficiency. B. subtilis natto fermentation product supplementation did not affect milk fat percentage and protein yield but increased (p < 0.05) milk fat yield and lactose percentage (p < 0.01) and tended to decrease protein percentage (p = 0.06). The findings show that B. subtilis natto fermentation product was effective in increasing lactation performance of early lactation dairy cows possibly by altering the rumen fermentation pattern without any negative effects on blood metabolites.

The effects of probiotics administration on the milk production, milk components and fecal bacteria microbiota of dairy cows

“…Many studies have demonstrated that certain microorganisms can exert beneficial effects to the host and thus boost the production performance of dairy cows [21][22][23]. Furthermore, administering probiotics can help maintain the balance of gut microbiota and even improve the ruminal bacterial composition [1,15].…”

Farklı Miktarlarda Bacillus Coagulans’ın Mısır Küspesi ve Pirinç Samanının Fermantasyon Özelliklerine Etkisinin In Vitro Değerlendirilmesi

“…crude fiber, starch and soluble sugar) to pyruvic acid, which could be transferred into different VFAs by metabolic pathways. Many researches have been conducted to verify that VFAs produced from the rumen could provide 50-80% energy needed by ruminants [33] . Our result showed that individual VFA and TVFA contents of maize stover were significantly higher than that of rice straw, it might result from the differences of fiber content and its continents, starch content and other carbohydrates between two fermentation substrates, and then affected the microorganism activities or the activation of microbial enzyme to alter fermentation model.…”

Response of ruminant health and growth to the probiotic Bacillus amyloliquefaciens

“…By contrast, supplementation with similar probiotics such as Bacillus subtilis decreased the A/P ratio in lactating cows (Peng et al 2012) and weaned calves (Sun et al 2011). Valerate and butyrate are preferentially metabolized in the epithelium of the rumen, therefore higher concentrations of these acids can stimulate the development of rumen papillae (Kristensen et al 1998;Shen et al 2005).…”

“…Supplementation with probiotics can increase milk yield and milk protein in lactating cows (Qiao et al 2010;Peng et al 2012) and lactating ewes (Kritas et al 2006). However, most studies have found no effects of probiotics on growth performance …”

“…Supplementation with probiotics can reduce the incidence of diarrhoea and increase the dry matter intake (DMI) and daily weight gain (DWG) of pre-weaned ruminants (Kowalski et al 2009;Frizzo et al 2010b;Sun et al 2010a). Probiotics can also increase milk production and milk quality in lactating cows (Qiao et al 2010;Peng et al 2012). By contrast, numerous other studies have shown no benefits to both ruminants and pre-weaned ruminants in response to their feeding with probiotics (Quintero-Gozalez et al 2003;Kumagai et al 2004;Bakhshi et al 2006;Riddell et al 2010).…”