Effect of Feeding Bacillus sp. As Probiotic Bacteria on Growth of Giant Freshwater Prawn (Macrobrachium rosenbergii de Man)

Abstract

The effects of feeding two Bacillus spp. isolated from the intestine of the giant freshwater prawn on the growth of Giant Freshwater Prawns (Macrobrachium rosenbergii de Man) was examined. The isolated Bacillus KKU02 and Bacillus KKU03 (approximately 10(7) CFU mL(-1)) were mixed into commercial prawn feed (200 mL kg(-1)). After rearing shrimp with the bacteria in four feed treatments, (Bacillus KKU03, Bacillus KKU02, mixed culture and control groups) for 120 days, body length and weight of the prawns in mixed culture tanks were significantly higher (p = 0.05) than in the control tanks (7.48 cm and 3.32 g, vs 6.6 cm and 2.1 g, respectively). Both isolates were found to produce amylase and protease. The stabilities of the single Bacillus sp., mixed culture and commercial probiotic in the feeds were examined during storage at 4 degrees C and room temperature. The percentage viability of Bacillus KKU02, Bacillus KKU03 and mixed culture stored at room temperature declined dramatically to 2.54, 21.88 and 10.92% within 2 weeks, respectively. At 4 degrees C however, the percentage viability of the tested probiotics reduced slowly. The survival of the commercial probiotics was the same at both temperatures about 50% after 70 days' storage.

Efficacy of Probiotics on Survival, Growth, Biochemical Changes and Energy Utilization Performance of <i>Macrobrachium rosenbergii</i> (De Man 1879) Post-larvae

“…These differences were found to be statistically significant (P<0.05). Similar results have been reported in M. rosenbergii fed with L. sporogenes supplemented diets [4], bio-encapsulated L. sporogenes [5], Binifit TM incorporated diets [6], Bacillus spp supplemented diets [7], Biogen® incorporated diets [8], different probiotics supplemented diets [9], Bacillus spp KKU02 and Bacillus spp KKU03 incorporated diets [11], bio-encapsulated B. subtilis [10], Saccharomyces cerevisiae and yeast derivatives supplemented diets [27], bio-encapsulated L. acidophilus and L. sporogenes [12], bio-encapsulated L. ceremoris [13] and in fishes, L. plantarum and B. megaterium supplemented diets [28] Lactobacillus acidophilus and yeast Saccharomyces cervisiae incorporated diets [29], B. subtilis, B. licheniformis and Enterococcus faecium incorporated diets [30], bio-encapsulated L. casei [31], Bacillus spp supplemented diets [32], B. subtilis supplemented diets [33] and B. toyoi and B. cereus incorporated diets [34].…”

Optimization of Production Parameters for Probiotic Lactobacillus Strains as Feed Additive

“…strains were more stable at a lower temperature (4 °C). Nevertheless, the survival of their isolates at room temperature after 28 days was actually lower than in our study, which can be assigned to a lower stability of liquid cultures compared to dried powders [61].…”

Potential role of prebiotics and probiotics in conferring health benefits in economically important crabs

“…and was defined by FAO/World Health Organization (WHO) as “live microorganisms which, when administered in adequate amounts, confer a health benefit on the host” [60] . In aquatic animals, several studies have documented the roles of probiotics in improving growth performance, contributing nutrition and enzymes to the digestion of the host, enhancing the host's immune responses, and controlling diseases [ [25] , [26] , [27] , [28] , [61] , [62] , [63] ]. The effectiveness of probiotics has been studied in crab species ( Table 1 ).…”