Effect of feeding a docosahexaenoic acid-enriched nutriceutical on the quality of fresh and frozen-thawed semen in Holstein bulls

Gholami, Hamid; Chamani, Mohammad; Towhidi, Armin; Fazeli, Mohammad Hashem

doi:10.1016/j.theriogenology.2010.06.025

Cited by 90 publications

(81 citation statements)

References 46 publications

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“…These effects were attributed only to antioxidant supplementation (SCHMID-LAUSIGK; AURICH, 2014). In the case of bulls, supplementation with only omega-3s resulted in improvements in the total and progressive motility, mean velocity of the trajectory, and HOSTpositive and proportion of fast spermatozoa in fresh semen, although there were no pronounced effects in frozen-thawed semen (GHOLAMI et al, 2010).…”

Section: Discussionmentioning

confidence: 90%

Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

Rodrigues

Ruiz

Nardo

et al. 2017

SCA

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For years, fatty acids have been recommended as a dietary supplement to improve canine hair. For animal reproduction, supplementation with omegas has been used to increase the reproductive efficiency and conception rate, but few studies have been conducted in dogs. The aim of this study was to evaluate the effects of daily dietary supplementation with omega-3 and -6 on the quality of fresh and frozen/ thawed semen in canines. Semen was collected from seven dogs and evaluated for sperm motility, vigor, concentration, and morphology. The 17-week study included 119 ejaculates and was divided according to oral supplementation with omega-3 and -6: M1 (1 st -5 th week) or pre-supplementation; M2 (6 th -9 th week) and M3 (10 th -13 th week) or during supplementation; and M4 (14 th -17 th week) or postsupplementation. After analysis, the semen was frozen and then revaluated both immediately and 30 minutes (at 37° C) after thawing. Supplementation with omegas increased sperm motility, vigor, and concentration; however, supplementation had no influence on semen freezability. In addition, there was no improvement in sperm motility after supplementation when the thawed cells were maintained at 37° C for 30 minutes. We concluded that dietary supplementation with omega-3 and -6 for 4 to 8 weeks can improve the quality of fresh semen, although it has no effect on the freezability of canine semen. Key words: Canine. Diet-supplemented. Polyunsaturated-fatty-acid. Sperm. ResumoOs ácidos graxos têm sido recomendados há anos como suplemento dietético para a melhora do pelo canino. Na área de reprodução animal, a suplementação com ômega tem sido usada para aumentar a eficiência reprodutiva e a taxa de concepção em muitas espécies, mas poucos estudos foram conduzidos em cães. Então, o objetivo deste estudo foi avaliar os efeitos da suplementação dietética diária com ômega-3 e -6 sobre a qualidade do sêmen fresco e congelado de cães. de 7 cães e avaliados quanto a motilidade espermática, vigor, concentração e morfologia. O estudo foi dividido de acordo com os períodos de suplementação oral com omegas-3 e -6, no qual M1 (1ª-5ª semana) foi considerado o período pré-suplementação, M2 (6ª-9ª semana) e M3 (10ª-13ª semana) período de suplementação e M4 (14ª-17ª semana) pós-suplementação, totalizando 17 semanas de estudo (119 ejaculados). Após a análise, o sêmen foi congelado e reavaliado imediatamente e 30 minutos após a descongelação (mantidas a 37º C). A suplementação resultou em aumento da motilidade espermática, vigor e concentração do sêmen fresco; no entanto, não houve influência na congelabilidade do sêmen. Além disso, não houve aumento da motilidade dos espermatozoides após a suplementação, quando as células descongeladas foram mantidas a 37° C, durante 30 minutos. Concluiu-se que a suplementação dietética com omegas-3 e -6, durante 4 a 8 semanas melhora os parâmetros do sêmen fresco de cães, embora não há nenhum efeito sobre a qualidade espermática após a congelação. Palavras-chave: Ácido-graxo-poli-insaturado. Canino. ...

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Section: Discussionmentioning

confidence: 90%

Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

Rodrigues

Ruiz

Nardo

et al. 2017

SCA

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“…However, higher amount of PUFAs increases the frozenthawed quality of sperm [9]. Dietary DHA improved motility of the fresh semen collected from bulls [10]. In vitro addition of DHA did not enhance the viability of frozen boars sperm [11].…”

Section: Introductionmentioning

confidence: 91%

Effect of in-vitro supplementation of polyunsaturated fatty acids on frozen-thawed bull sperm characteristics using Bioxcell® extender

Kaka¹

2016

PAB

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Cryopreservation is considered that it decreases sperm quality about 50 percent therefore research is being conducted to increase the frozen-thawed quality of bull sperm. The object of study was to examine the effect of polyunsaturated fatty acids on the frozen-thawed bull semen sperm characteristics. Semen was collected from three bulls with an average of 8 collection from each bull at interval of 3 days. Semen ejaculates with motility and normal morphology 70% and 80% respectively were processed in extender (BioXcell ® ) having combined supplementation 0 to 15ng/ml of alpha linolenic acid (ALA) and docosohexaenoic acid (DHA). Extended sample was cooled at 5 o C for 2 hours, packaged and frozen into straws and in liquid nitrogen for 24 hours. Sequentially, straws were examined for sperm cytological characteristics, lipid peroxidation, fatty acid concentration and super oxide dismutase (SOD) after thawing. Sperm cytological characteristics decreased in the supplemented groups in comparison to control group. Docosahexaenoic acid improved in all supplemented groups, whereas ALA improved at 5ng/ml of ALA. Superoxide dismutase and lipid peroxidation were improved linearly by increasing fatty acids. Conclusion, combination of ALA and DHA has detrimental effect on frozen-thawed bull sperm parameters.

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“…No presente estudo, não foi analisado o perfil de ácidos graxos na membrana plasmática dos espermatozoides. No entanto, supõe-se que o aumento do BCF para os animais tratados indica a possibilidade de haver maior incorporação de UFAs na membrana espermática e, por isso, ter havido aumento da intensidade dos batimentos, como reportado para bovinos (GHOLAMI et al, 2010) Apesar da linearidade dos espermatozoides do grupo OP ter diminuído no Período IV, os níveis obtidos (63,7%) são semelhantes aos níveis mais adequados de linearidade para fertilização (COX et al, 2002), pois estudos realizados com sêmen caprino demonstram que espermatozoides com valor médio para LIN de 60±1,3% foram capazes de percorrer maiores distâncias no muco cervical in vitro (P<0,05) quando comparados aos ejaculados que possuíam 66,6±1,0% e 58±1,4% de linearidade no movimento espermático (COX et al, 2006). Sabe-se que a viscosidade do meio interfere na livre circulação dos espermatozoides pelo plasma seminal (CASTELLINI et al, 2000).…”

Section: Pi_h342 (%) E_n (%)unclassified

“…Esses valores são próximos dos 20% de defeitos espermáticos totais observados no sêmen in natura de búfalos, quando suplementados com uso de UFAS (SILVA et al, 2014), o que sugere que a suplementação lipídica pode exercer efeito protetor em ejaculados que devam passar pelo processo de criopreservação. O uso de lipídios na dieta presumivelmente não incrementou a deposição de gordura peritesticular, sendo um fator que influenciaria na termorregulação testicular (GHOLAMI et al, 2010) e poderia determinar marcante elevação na quantidade de patologias de cauda no ejaculado, o que ocorre quando a temperatura testicular está acima de 32ºC em búfalos (OHASHI et al, 1988).…”

Section: Pi_h342 (%) E_n (%)unclassified

“…Não houve diferença nos níveis de integridade de membrana plasmática avaliados pelas técnicas de HOST e eosina-nigrosina, corroborando resultados encontrados para sêmen bovino descongelado, proveniente de touros alimentados com dietas enriquecidas com ácidos graxos poli-insaturados (GHOLAMI et al, 2010). Quando caprinos foram alimentados com óleo de peixe, não foi encontrada diferença significativa para integridade de membrana plasmática, avaliada pelo teste de coloração com eosina-nigrosina (DOLATPANAH et al, 2008).…”

Section: Pi_h342 (%) E_n (%)unclassified

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A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

et al. 2014

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This research aimed to evaluate the effect of inclusion unsaturated fatty acids (UFAs) in the buffalo diet, on the sperm quality after thawing. In parallel, it was verified the degree of association among techniques to assess plasma membrane integrity (PMI) of spermatozoa. Patterns of sperm motility (subjective and computer-assisted analysis), kinetics, morphology and plasma membrane integrity were evaluated. The hypo-osmotic swelling test, nigrosin-eosin staining (E-N) and fluorescent probes propidium iodide/Hoechst 33342 (IP-H342) were adopted to evaluate PMI. The study was planned considering two experimental groups, which were evaluated in repeated measures design. over time. Eleven Murrah buffalo bulls (3.5±1.0 years, and 536.9±73.0 kg) were divided in Control Group (CONT, n=5) and Palm Oil Group (PO, n=6). Animals were fed with forage and concentrated in a controlled manner. Animals of PO received dietary supplementation containing palm oil (2% of dry matter). Dietary consumption was daily monitored and the intake level of UFAs in PO was 55.4% higher than CONT. Semen samples were collected and evaluated every 14 days. The experiment lasted 120 days, considering four periods of 30 days each (Periods I, II, III and IV) and repeated measures structure was used in statistical analysis. Significance level adopted was 5%. There was no effect of treatment on sperm motility (P>0.05), but palm oil supplementation presented a positive effect on spermatic ability to progress (derived from BCF, STR and LIN) in Period IV (P<0.05). High correlation (91%) was observed between IP-H342 and E-N techniques (Y = 0.9155 x + 2.9843, R 2 = 0.82, P≤0.05). Thus, supplementation with UFAs increased the progressivity of spermatozoa after 90 days, and had no effect on other seminal parameters. Eosin-nigrosin stain can be used efficiently as a predictor of the level of PMI when conditions for implementing fluorescent probes not present.

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Effect of feeding a docosahexaenoic acid-enriched nutriceutical on the quality of fresh and frozen-thawed semen in Holstein bulls

Cited by 90 publications

References 46 publications

Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

Effect of in-vitro supplementation of polyunsaturated fatty acids on frozen-thawed bull sperm characteristics using Bioxcell® extender

A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

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