Effect of fecal microbiota transplantation on the TGF-β1/Smad signaling pathway in rats with TNBS-induced colitis

Abstract

Background Traditional treatments for inflammatory bowel disease (IBD) have adverse side effects, and patients who receive such treatments have high recurrence rates. Fecal microbiota transplantation (FMT) has become an increasingly popular therapeutic option for patients with IBD. However, the mechanism by which FMT alleviates this disease remains unclear. Methods In this study, a rat model of 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis was established and used to explore whether the transforming growth factor-beta 1 (TGF-β1)/small mothers against decapentaplegic (Smad) signaling pathway plays a critical role in the FMT alleviation of IBD. Results After the FMT intervention, the disease activity index and histologic scores were significantly decreased. In addition, the TGF-β1 expression level in the FMT group was significantly decreased by approximately 0.72-fold relative to the level in the TNBS colitis group, whereas the Smad3, Smad4, and Smad7 expression levels had increased by approximately 1.21, 1.40, and 1.18 folds, respectively. Similarly, SB431542 inhibited the expression of TGF-β1 and promoted the expression of Smad3, Smad4, and Smad7. Further, the serum levels of the inflammatory cytokines tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were significantly decreased, whereas that of the interferon-gamma (IFN-γ) was not significantly changed after the FMT intervention. Conclusions These results suggest that FMT inhibits the TGF-β1/Smad signaling pathway to attenuate inflammation.

Voluntary Exercise-Mediated Protection in TNBS-Induced Rat Colitis: The Involvement of NETosis and Prdx Antioxidants

“…Furthermore, we found an elevated level of TGF-β cytokine in TNBS-treated groups compared to controls. In accordance with our results, Qui et al [ 39 ] showed that TNBS treatment significantly elevated TGF-β expression in rats, and Del Zotto et al [ 40 ] found the same alteration in the colonic tissue of IBD patients. Furthermore, Silva et al [ 41 ] found that chronic exercise significantly attenuated TGF-β levels in middle-aged obese mice.…”

When less is more: Experimental Bishop–Koop technique for reduction in the use of laboratory animals for intestinal pathophysiological studies

“…Among experimental studies focused on gastrointestinal diseases found in the literature, IIRI-and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats appears as a current model used by many authors and research groups around the world. [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] For this reason, we selected these two relevant pathologies to evaluate the experimental BK procedure in an intestinal injury scenario. IIRI was used for simulating a model of intestinal intussusception, strangulated hernia or intestinal transplantation.…”

“…[15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] The same situation occurs in the field of experimental colitis, where old and recent scientific articles use one animal in each LI sample collection. [30][31][32][33][34][35][36][37] For the reasons mentioned above, it is a challenge to develop models that enable reducing the number of animals involved in these studies.…”

Efectos del precondicionamiento isquémico abdominal multiorgánico en modelos experimentales de isquemia-reperfusión

“…Este es un modelo experimental de interés de investigación en la actualidad entre las patología de intestino grueso utilizado por múltiples autores y grupos de investigación. (104)(105)(106)(107)(108)(109)(110)(111) Materiales y métodos: Se constituyeron tres grupos compuestos por ratas Sprague-Dawley hembras (peso 190 ± 2,6 gr). A las cuales se les realizó la técnica de BK en la porción distal del intestino grueso.…”