Effect of fatty acid membrane composition on whole-cell biocatalysts for biodiesel-fuel production

Hama, Shinji; Yamaji, Hideki; Kaieda, Masaru; Oda, Mitsuhiro; Kondo, Akihiko; Fukuda, Hideki

doi:10.1016/j.bej.2004.05.009

Cited by 93 publications

(30 citation statements)

References 20 publications

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“…Furthermore, the basal medium used in two-step cultivation method contained 30% olive oil which is consisted of 55-83% oleic acid. The unsaturated fatty acid existence in medium broth altered cell membrane permeability become more permeable [1] inducing lipase secretion.…”

Section: Resultsmentioning

confidence: 99%

“…In the past decade, biodiesel has attracted considerable attention as a renewable, biodegradable and non-toxic fuel [1]. Current technology for biodiesel production, using an alkali or an acid catalyst, has several important limitations such as requires feedstock free of water, produce salts, generate glycerin as a low-grade by-product, etc [2].…”

Section: Introductionmentioning

confidence: 99%

“…Whole-cells are frequently entrapped in biomass support particles (BSPs) [1], [10]- [15]. In this research, we also immobilized whole-cells of R. oryzae on BSP.…”

Section: Introductionmentioning

confidence: 99%

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Kinetic Study of Immobilized Whole-Cell Lipase Rhizopus Oryzae as Biocatalyst in Biodiesel Synthesis through Non-Alcohol Route

Hermansyah¹,

Faiz²,

Arbianti³

2015

IJESD

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Abstract-Utilizing whole-cell biocatalyst is a potential way to reduce catalyst cost in biodiesel production using lipase as catalyst. Whole-cell of Rhizopus oryzae was cultivated by one-step and two-step method and was immobilized on Biomass Support Particles (BSPs) and chitosan-TPP bead. Immobilized whole-cells on BSPs produce 11% (one-step) and 12% (two-step) FAME yield. While, FAME yield produced by immobilized whole-cell in chitosan-TPP beads are 23% (one-step) and 22% (two-step).

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Kinetic Study of Immobilized Whole-Cell Lipase Rhizopus Oryzae as Biocatalyst in Biodiesel Synthesis through Non-Alcohol Route

Hermansyah¹,

Faiz²,

Arbianti³

2015

IJESD

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“…Além disto, células provenientes de meio enriquecido com ácido palmítico mostraram maior estabilidade do que células enriquecidas com ácidos graxos insaturados. 105 O cultivo na presença e ausência de óleo de oliva também apresentou efeito sobre a atividade lipolítica das células íntegras de Aspergillus niger, conforme verificado por Romero et al, 70 . As especificidades dos biocatalisadores variaram com o tipo de reação e o substrato utilizado (ácido graxo, éster de ácido graxo ou álcool).…”

Section: Condições Para a Produção De Lipases Ligadas Ao Micélio De Funclassified

Potencial catalítico de lipases ligadas ao micélio de fungos filamentosos em processos de biotransformação

2016

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“…Several works have been reported concerning biodiesel production using whole-cells derived from Rhizopus oryzae (Hama et al, 2004;Ban et al, 2008), Pseudomonas fragi (Schuepp et al, 1997), Penicillium cyclopium (Legier and Comeau, 1992), Aspergillus niger (Xiao et al, 2010;Romero et al, 2012) and Mucor circinelloides (Antczak et al, 2002;Szczesna-Antczak et al, 2004;Andrade et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization and Application of a Polyurethane-Based Support for Immobilizing Membrane-Bound Lipase

Soares

Rico

Andrade

et al. 2017

Braz. J. Chem. Eng.

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-This study conducted an assessment of polyurethane foams that were synthesized by one-shot process and used as a low-cost support to immobilize Mucor circinelloides URM 4182 whole-cells presenting high lipolytic activity. Polyols with different molecular weights (1100 to 6000 g mol ) were applied to synthesize the polymer matrix, and the agitation speed effect was used for controlling the average pore size of the investigated polyurethane foams. The physical and mechanical properties of the polymers were evaluated by standard test methods, and their morphology was identified by Scanning Electron Microscopy. The immobilization procedure efficiency was assessed by quantifying the capability of the matrices to attach the cells and the catalytic activity of the biocatalysts in both aqueous (olive oil hydrolysis) and non-aqueous media (ethanolysis of babassu oil) under single and consecutive batch runs. Although all synthesized matrices were suitable to immobilize the whole cells with high catalytic performance, a better set of parameters was attained when the polyol ether with molecular weight of 6000 g mol -1and 1100 g mol -1 was used. Both matrices yielded immobilized biocatalysts with high hydrolysis and transesterification activities, and exhibited a satisfactory operational stability with 96% and 81% retention of their initial hydrolytic and transesterification activities after three consecutive batch runs.

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Effect of fatty acid membrane composition on whole-cell biocatalysts for biodiesel-fuel production

Cited by 93 publications

References 20 publications

Kinetic Study of Immobilized Whole-Cell Lipase Rhizopus Oryzae as Biocatalyst in Biodiesel Synthesis through Non-Alcohol Route

Kinetic Study of Immobilized Whole-Cell Lipase Rhizopus Oryzae as Biocatalyst in Biodiesel Synthesis through Non-Alcohol Route

Potencial catalítico de lipases ligadas ao micélio de fungos filamentosos em processos de biotransformação

Synthesis, Characterization and Application of a Polyurethane-Based Support for Immobilizing Membrane-Bound Lipase

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