Effect of fatty acid-free bsa, replacement of serum supplementation in culture medium on the biopsyand subsequent cryopreservation of in vitro-produced bovine embryos

Yotsushima, Kenji; Shimizu, Morihito; Sakaguchi, Minoru; Kakei, Tomoko; Izaike, Yoshiaki

doi:10.1016/s0093-691x(99)91738-7

Cited by 1 publication

(4 citation statements)

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“…After this, embryos were transferred to vitrification solution (20% glycerol + 20% ethylene glycol + 0.3 M trehalose + 10% polyvinyl pyrrolidone in mPBS) for 2 min at 4 C, followed by plunging into LN2. Vitrified embryos were warmed in a water bath at 20 C and expelled into mPBS containing 0.5 M sucrose and 20% FCS [9].…”

Section: Cryopreservation Of Reconstituted Embryosmentioning

confidence: 99%

“…Vitrification has been demonstrated to be an adequate technique for cryopreserving in vitro produced embryos [13] and biopsied embryos [9]. Thus, it is suggested that vitrification rather than slow cooling is also suitable for cryopreservation of reconstituted embryos derived from fetal cells.…”

Section: Embryo Survivalmentioning

confidence: 99%

“…It has therefore been demonstrated that NT-calves derived from fetal fibroblast cells could grow healthily with normal growth rates. shows the average growth of female Japanese Black cattle [9].…”

Section: Parturition and Characteristics Of Nt-calvesmentioning

confidence: 99%

“…[8]. Cryopreservation is important for reconstituted embryos as well as for manipulated embryos for sexing [9]. In this study, the viability of reconstituted embryos derived from female fetal cells after transfer to term was investigated and resulted in the production of -Research Noteollowing the first report of viable offspring being obtained by the transfer of reconstituted embryos derived from fetal and mammary gland epithelial cells in sheep [1], this cloning method using somatic cells has since been successfully applied to cattle [2][3][4].…”

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Cloned Calves Produced by Transfer of Reconstituted Embryos Derived from Fibroblast Cells of a Female Fetus.

Sakaguchi

Yotsushima

Kakei

et al. 2000

Journal of Reproduction and Development

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Abstract. The viability of reconstituted embryos derived from bovine somatic cells were evaluated. A total of 54 fetal somatic cell-derived embryos, in which some embryos were previously cryopreserved, were transferred to 12 recipient cattle (total 26 times). Three of 12 cattle that received fresh embryos became pregnant and delivered 3 calves (1 aborted at 223d and 2 live born), whereas none out of the 5 recipients became pregnant after transfer of embryos cryopreserved by slow cooling. Of 9 cattle that received embryos vitrificated 2 became pregnant. One of these two vitrification-derived embryos died during early pregnancy and another developed to term but was stillborn. These aborted fetuses, stillborn calf and two live born calves were identified as being derived from donor cells. The growth of live born calves showed no abnormalities when examined at 8 months of age. It was therefore concluded that: reconstituted embryos derived from fibroblast cells from female fetus could develop to term, that vitrification might be available to preserve these embryos and that transfer of these fresh embryos resulted in viable offspring with normal growth.

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Section: Cryopreservation Of Reconstituted Embryosmentioning

confidence: 99%

Section: Embryo Survivalmentioning

confidence: 99%

Section: Parturition and Characteristics Of Nt-calvesmentioning

confidence: 99%

mentioning

confidence: 99%

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