Effect of fasting on digestive gland lipase transcripts expression in Penaeus vannamei

Rivera-Pérez, Crisalejandra; García‐Carreño, Fernando

doi:10.1016/j.margen.2011.07.002

Cited by 29 publications

(17 citation statements)

References 37 publications

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“…digestive and intracellular lipases. Digestive lipase is exclusively expressed in the hepatopancreas, suggesting that they function as a digestive enzyme, while intracellular lipase was found in the pleopods, digestive tube, uropods, hemocytes, muscle and gonad, indicating it functions in the mobilization of energy reserves [39]. Because of higher mRNA expression levels of triacylglycerol lipases in P. trituberculatus hepatopancreas, it is suggested that this triacylglycerol lipase is likely a digestive lipase for P. trituberculatus .…”

Section: Resultsmentioning

confidence: 98%

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Insights into Hepatopancreatic Functions for Nutrition Metabolism and Ovarian Development in the Crab Portunus trituberculatus: Gene Discovery in the Comparative Transcriptome of Different Hepatopancreas Stages

et al. 2014

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The crustacean hepatopancreas has different functions including absorption, storage of nutrients and vitellogenesis during growth, and ovarian development. However, genetic information on the biological functions of the crustacean hepatopancreas during such processes is limited. The swimming crab, Portunus trituberculatus, is a commercially important species for both aquaculture and fisheries in the Asia-Pacific region. This study compared the transcriptome in the hepatopancreas of female P. trituberculatus during the growth and ovarian maturation stages by 454 high-throughput pyrosequencing and bioinformatics. The goal was to discover genes in the hepatopancreas involved in food digestion, nutrition metabolism and ovarian development, and to identify patterns of gene expression during growth and ovarian maturation. Our transcriptome produced 303,450 reads with an average length of 351 bp, and the high quality reads were assembled into 21,635 contigs and 31,844 singlets. Based on BLASTP searches of the deduced protein sequences, there were 7,762 contigs and 4,098 singlets with functional annotation. Further analysis revealed 33,427 unigenes with ORFs, including 17,388 contigs and 16,039 singlets in the hepatopancreas, while only 7,954 unigenes (5,691 contigs and 2,263 singlets) with the predicted protein sequences were annotated with biological functions. The deduced protein sequences were assigned to 3,734 GO terms, 25 COG categories and 294 specific pathways. Furthermore, there were 14, 534, and 22 identified unigenes involved in food digestion, nutrition metabolism and ovarian development, respectively. 212 differentially expressed genes (DEGs) were found between the growth and endogenous stage of the hepatopancreas, while there were 382 DEGs between the endogenous and exogenous stage hepatopancreas. Our results not only enhance the understanding of crustacean hepatopancreatic functions during growth and ovarian development, but also represent a basis for further research on new genes and functional genomics of P. trituberculatus or closely related species.

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Section: Resultsmentioning

confidence: 98%

“…Triacylglycerol lipases, generally named lipases, play an important physiological role, and are involved in triacylglyceride catabolism of crustaceans and mammals [38], [39]. Triacylglycerol lipases can be divided into two classes, i.e.…”

Section: Resultsmentioning

confidence: 99%

Insights into Hepatopancreatic Functions for Nutrition Metabolism and Ovarian Development in the Crab Portunus trituberculatus: Gene Discovery in the Comparative Transcriptome of Different Hepatopancreas Stages

et al. 2014

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“…Molting involves several stages with different feeding behaviors, including the cessation of external food intake from late premolt through early postmolt; therefore, energy needs can be met with different available external food sources or lipid reserves. Digestive enzymes are used as a physiological response to fasting (Cuzon et al 1980, Jones & Obst 2000, MuhliaAlmazán & García-Carreño 2002, Rivera-Pérez & García-Carreño 2011, Calvo et al 2013. Artificiallyinduced fasting and starvation may allow elucidation of the metabolic routes used in hierarchical order during molting and may initiate alternative biochemical and physiological adaptation mechanisms (Barclay et al 1983, Comoglio et al 2008.…”

Section: Introductionmentioning

confidence: 99%

Effect of attractant stimuli, starvation period and food availability on digestive enzymes in the redclaw crayfish Cherax quadricarinatus (Parastacidae)

Sacristán¹,

Nolasco-Soria²,

Greco³

2014

Aquat. Biol.

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“…The activity of lipases registered in the two species studied would account for the activity of the digestive lipase, which is found in the hepatopancreas, as well as the intracellular lipase (lysosomal). The former is responsible for hydrolysing the lipids of the diet, while the latter is responsible for the hydrolysis of the reserve triacylglycerols (Rivera‐Pérez & García‐Carreño, ). Both enzymes prove to be constantly active in all sizes of organisms, acting on triacylglycerols from the diet and also in those that allow it to obtain energy to be used or transferred as reserve material.…”

Section: Discussionmentioning

confidence: 99%

“…The former is responsible for hydrolysing the lipids of the diet, while the latter is responsible for the hydrolysis of the reserve triacylglycerols (Rivera-Pérez & García-Carreño, 2011). Both enzymes prove to be constantly active in all sizes of organisms, acting on triacylglycerols from the diet and also in those that allow it to obtain energy to be used or transferred as reserve material.…”

Section: Discussionmentioning

confidence: 99%

Development of enzymes during ontogeny of two freshwater Decapoda:Aegla uruguayana(Aeglidae) andMacrobrachium borellii(Palaemonidae)

et al. 2018

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The similarities or differences among species of the same family or among different families suggest a relationship with their feeding habits or a preference for the various types of food. The aim of this study was to identify the presence of digestive enzymes lipases, total protease, amylases and cellulose, and the effects of sex and size in these activities in two decapod freshwater crustaceans. Specimens of different sizes and sexes of Aegla uruguayana and Macrobrachium borellii were collected in the natural environment. In laboratory, enzymatic preparation consisted in homogenising the digestive gland in a buffer Tris‐HCl. The homogenates were centrifuged and the lipid layer was removed. Enzyme activities were determined using standard protocols. There were no statistically significant differences between sexes in any of the species under study. However, there were changes in the activity of amylases during the ontogeny of A. uruguayana, indicating that in juveniles the values of said activity are significantly higher than in the rest of the groups. M. borellii showed ontogenetic differences in total proteinase activity: organisms of range 2 (intermediate sizes) presented enzymatic activities much smaller than organisms of range 4 (larger sizes). The comparisons of enzymatic activities between both species yielded significant differences, being higher the values in the species A. uruguayana with respect to M. borellii. The results obtained in this work indicate that although both species are omnivorous and consume similar items, the activity of these digestive enzymes could be an evidence of different strategies of utilisation of the resource.

show abstract

Effect of fasting on digestive gland lipase transcripts expression in Penaeus vannamei

Cited by 29 publications

References 37 publications

Insights into Hepatopancreatic Functions for Nutrition Metabolism and Ovarian Development in the Crab Portunus trituberculatus: Gene Discovery in the Comparative Transcriptome of Different Hepatopancreas Stages

Insights into Hepatopancreatic Functions for Nutrition Metabolism and Ovarian Development in the Crab Portunus trituberculatus: Gene Discovery in the Comparative Transcriptome of Different Hepatopancreas Stages

Effect of attractant stimuli, starvation period and food availability on digestive enzymes in the redclaw crayfish Cherax quadricarinatus (Parastacidae)

Development of enzymes during ontogeny of two freshwater Decapoda:Aegla uruguayana(Aeglidae) andMacrobrachium borellii(Palaemonidae)

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