Effect of fabrication parameters on morphology and drug loading of polymer particles for rosiglitazone delivery

Spetz, Madeline R.; Isely, Christopher; Gower, R. Michael

doi:10.1016/j.jddst.2021.102672

Cited by 1 publication

(2 citation statements)

References 37 publications

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“…PLG particles were formed using a single oil-in-water emulsification followed by the solvent extraction method, reported previously by our group. − Briefly, 50:50 PLG was dissolved in dichloromethane at 4% (wt/wt). An amount of 0.6 mL of the 4% PLG solution was added dropwise to 4 mL of an aqueous solution of 0.2% (wt/v) poly(vinyl alcohol) (PVA) or modified PVA and emulsified at 11,000 rpm for 5 min using a Kinematica PT3100D homogenizer.…”

Section: Methodsmentioning

confidence: 99%

“…For these studies, this value ranged from 0.1 to 20 mol %. The solution was stirred continuously at a predetermined temperature (25,60,80, or 100 °C) for either 5 or 24 h. Then, the solution was added to dialysis tubing with a 3.5 kDa molecular weight cutoff (MWCO) and dialyzed (protected from light) against 5 L of ultrapure water for 3 days, with exchanges of water twice a day. Following dialysis, the PVA product where M product is the mass of PVA recovered from the reaction, M PVA is the initial mass of PVA, and M ITC is the initial mass of isothiocyanate.…”

Section: Modification Of Poly(vinyl Alcohol) (Pva)mentioning

confidence: 98%

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Surface Functionalization of Polymer Particles for Cell Targeting by Modifying Emulsifier Chemistry

Isely

Atube

Cheung

et al. 2022

ACS Appl. Polym. Mater.

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The oil-in-water emulsion/solvent extraction method is used to fabricate many FDA approved, polymer particle formulations for drug delivery. However, these formulations do not benefit from surface functionalization that can be achieved by tuning particle surface chemistry. Poly(vinyl alcohol) (PVA) is the emulsifier used for many FDA approved formulations and remains associated with the particle surface after fabrication. We hypothesized that the hydroxyl groups in PVA could be conjugated with biomolecules using isothiocyanate chemistry and that these modifications would endow the particle surface with additional functionality. We demonstrate that fluorescein isothiocyanate and an isothiocyanate derivatized mannose molecule can be covalently attached to PVA in a one-step reaction. The modified PVA polymers perform as well as unmodified PVA in acting as an emulsifier for fabrication of poly(lactide-co-glycolide) particles. Particles made with fluorescein-modified PVA exhibit fluorescence confined to the particle surface, while particles made with mannosemodified PVA bind concanavalin A. In addition, mannose-modified PVA increases particle association with primary macrophages by threefold. Taken together, we present a facile method for modifying the surface reactivity of polymer particles widely used for drug delivery in basic research and clinical practice. Given that methods are established for conjugating the isothiocyanate functional group to a wide range of biomolecules, our approach may enable PVA-based biomaterials to engage a multitude of biological systems.

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Section: Methodsmentioning

confidence: 99%

Section: Modification Of Poly(vinyl Alcohol) (Pva)mentioning

confidence: 98%